Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(...Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(6'-SL),which is known to beneficially influence neural functions.Using Sambucus nigra lectin,which specifically binds to 6'-SL,we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids.Mimetic peptide,reverse peptide and scrambled peptide were tested for inhibition of 6'-SL binding to the lectin.Indeed,lectin binding to 6'-SL was inhibited by the most frequently identified mimetic peptide,but not by the reverse or scrambled peptides,showing that this peptide mimics 6'-SL.Functionally,mimetic peptide,but not the reverse or scrambled peptides,increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells,and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H_20_2-induced oxidative stress.The combined results indicate that the 6'-SL mimetic peptide promotes neuronal survival and neuritogenesis,thus raising hopes for the treatment of neurodegenerative diseases.This study was approved by the Medical Ethics Committee of Shantou University Medical College,China(approval No.SUMC 2014-004)on February 20,2014.展开更多
目的:研究乳腺良恶性病变组织中β-半乳糖凝集素-3(galectin-3,gal-3)和接骨木凝集素(Sambucus nigra agglutinin,SNA)表达水平及其临床病理意义。方法:60例乳腺癌和30例乳腺良性病变组织常规制作石蜡包埋切片,gal-3染色方法为EnVsionT...目的:研究乳腺良恶性病变组织中β-半乳糖凝集素-3(galectin-3,gal-3)和接骨木凝集素(Sambucus nigra agglutinin,SNA)表达水平及其临床病理意义。方法:60例乳腺癌和30例乳腺良性病变组织常规制作石蜡包埋切片,gal-3染色方法为EnVsionTM免疫组织化学法和SNA染色法为ABC亲和免疫组织化学法。结果:乳腺癌gal-3和SNA阳性表达均为29例(48.3%),其评分值分别为2.07±2.25和2.12±2.26;乳腺良性病变gal-3和SNA阳性表达均为8例(26.7%),两者评分值分别为1.03±1.63和1.07±1.59;乳腺癌gal-3和SNA表达阳性率(χ2=3.89,P=0.048)及其评分(t=2.24,P=0.028;t=2.33,P=0.022)明显高于乳腺良性病变。雌激素受体(ER)阳性及CA15-3阴性病例gal-3和SNA表达评分明显低于ER阴性和CA15-3阳性病例(P<0.05或P<0.01);经Kaplan-Meier生存分析发现gal-3和SNA表达阳性患者术后5年生存率和平均生存期明显低于其阴性患者(P<0.05或P<0.01)。结论:Gal-3和SNA的表达对乳腺癌的发生发展和临床生物学行为可能有重要作用,gal-3和/或SNA阳性表达的乳腺癌提示预后不良。展开更多
目的研究胆囊良恶性病变组织中β-半乳糖凝集素-3（galectin-3，gal-3）和接骨木凝集素（sambucus nigra agglutinin，SNA）表达水平及其临床病理意义。方法108例胆囊腺癌、46例癌旁组织、15例腺瘤性息肉和35例慢性胆囊炎组织常规制作...目的研究胆囊良恶性病变组织中β-半乳糖凝集素-3（galectin-3，gal-3）和接骨木凝集素（sambucus nigra agglutinin，SNA）表达水平及其临床病理意义。方法108例胆囊腺癌、46例癌旁组织、15例腺瘤性息肉和35例慢性胆囊炎组织常规制作石蜡包埋切片。gal-3染色方法为EnVsion^TM免疫组织化学法，SNA染色法为ABC亲和组化法。结果胆囊腺癌gal-3和SNA表达阳性率（62．0％，66．7％）明显高于癌旁组织（39．1％，45．6％）、腺瘤性息肉（26．7％，33．3％）和慢性胆囊炎胆囊上皮（11．4％，11．4％），组间差异具有统计学意义（P〈0．05或P〈0．01）；gal-3和SNA表达阳性的良性病变胆囊上皮均呈不典型增生；高分化腺癌、肿块最大径〈2cm、无淋巴结转移及未侵犯周围组织病例gal-3和SNA表达阳性率均明显低于低分化腺癌、肿块最大径≥2cm、淋巴结转移及侵犯周围组织病例，组间差异具有统计学意义（P〈O．05或P〈0．01）；胆囊腺癌中gal-3和SNA表达水平呈高度一致性（X^2-9．51，P％0．01）。结论gal-3和SNA表达水平在胆囊腺癌发生、进展、临床生物学行为方面可能有重要作用，gal-3和（或）SNA阳性表达的胆囊腺癌可能预后不良。展开更多
基金supported by the National Natural Science Foundation of China,No.81471279 and No.81171138(to WJZ)Talent Support Grant from Shantou University Medical College,China,No.2501220118(to WJZ)the Li Kashing Foundation,No.LD030302(to MS)
文摘Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(6'-SL),which is known to beneficially influence neural functions.Using Sambucus nigra lectin,which specifically binds to 6'-SL,we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids.Mimetic peptide,reverse peptide and scrambled peptide were tested for inhibition of 6'-SL binding to the lectin.Indeed,lectin binding to 6'-SL was inhibited by the most frequently identified mimetic peptide,but not by the reverse or scrambled peptides,showing that this peptide mimics 6'-SL.Functionally,mimetic peptide,but not the reverse or scrambled peptides,increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells,and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H_20_2-induced oxidative stress.The combined results indicate that the 6'-SL mimetic peptide promotes neuronal survival and neuritogenesis,thus raising hopes for the treatment of neurodegenerative diseases.This study was approved by the Medical Ethics Committee of Shantou University Medical College,China(approval No.SUMC 2014-004)on February 20,2014.
文摘目的:研究乳腺良恶性病变组织中β-半乳糖凝集素-3(galectin-3,gal-3)和接骨木凝集素(Sambucus nigra agglutinin,SNA)表达水平及其临床病理意义。方法:60例乳腺癌和30例乳腺良性病变组织常规制作石蜡包埋切片,gal-3染色方法为EnVsionTM免疫组织化学法和SNA染色法为ABC亲和免疫组织化学法。结果:乳腺癌gal-3和SNA阳性表达均为29例(48.3%),其评分值分别为2.07±2.25和2.12±2.26;乳腺良性病变gal-3和SNA阳性表达均为8例(26.7%),两者评分值分别为1.03±1.63和1.07±1.59;乳腺癌gal-3和SNA表达阳性率(χ2=3.89,P=0.048)及其评分(t=2.24,P=0.028;t=2.33,P=0.022)明显高于乳腺良性病变。雌激素受体(ER)阳性及CA15-3阴性病例gal-3和SNA表达评分明显低于ER阴性和CA15-3阳性病例(P<0.05或P<0.01);经Kaplan-Meier生存分析发现gal-3和SNA表达阳性患者术后5年生存率和平均生存期明显低于其阴性患者(P<0.05或P<0.01)。结论:Gal-3和SNA的表达对乳腺癌的发生发展和临床生物学行为可能有重要作用,gal-3和/或SNA阳性表达的乳腺癌提示预后不良。
文摘目的研究胆囊良恶性病变组织中β-半乳糖凝集素-3（galectin-3，gal-3）和接骨木凝集素（sambucus nigra agglutinin，SNA）表达水平及其临床病理意义。方法108例胆囊腺癌、46例癌旁组织、15例腺瘤性息肉和35例慢性胆囊炎组织常规制作石蜡包埋切片。gal-3染色方法为EnVsion^TM免疫组织化学法，SNA染色法为ABC亲和组化法。结果胆囊腺癌gal-3和SNA表达阳性率（62．0％，66．7％）明显高于癌旁组织（39．1％，45．6％）、腺瘤性息肉（26．7％，33．3％）和慢性胆囊炎胆囊上皮（11．4％，11．4％），组间差异具有统计学意义（P〈0．05或P〈0．01）；gal-3和SNA表达阳性的良性病变胆囊上皮均呈不典型增生；高分化腺癌、肿块最大径〈2cm、无淋巴结转移及未侵犯周围组织病例gal-3和SNA表达阳性率均明显低于低分化腺癌、肿块最大径≥2cm、淋巴结转移及侵犯周围组织病例，组间差异具有统计学意义（P〈O．05或P〈0．01）；胆囊腺癌中gal-3和SNA表达水平呈高度一致性（X^2-9．51，P％0．01）。结论gal-3和SNA表达水平在胆囊腺癌发生、进展、临床生物学行为方面可能有重要作用，gal-3和（或）SNA阳性表达的胆囊腺癌可能预后不良。
