Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsm...Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsmorphology, physiological and biochemical properties, cellwall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 wassequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was namedSaccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilatedpyrene for growth and 0.005% of yeast extract stimulatedpyrene degradation and assimilation. The optimal pH ofcultural medium and the optimal shaking frequency during cultivation were 6—8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacteriumsp. PYR-1.展开更多
【目的】用生防菌Hhs.015发酵液处理苹果发病枝条,研究其对树体微生态的影响。【方法】采用改良CTAB法提取样本中的总DNA,对细菌群落的16S r DNA的V4+V5区进行高通量测序,研究发病枝条涂抹Hhs.015菌悬液30 d后树皮内细菌区系组成变化,...【目的】用生防菌Hhs.015发酵液处理苹果发病枝条,研究其对树体微生态的影响。【方法】采用改良CTAB法提取样本中的总DNA,对细菌群落的16S r DNA的V4+V5区进行高通量测序,研究发病枝条涂抹Hhs.015菌悬液30 d后树皮内细菌区系组成变化,分析其相对丰度以及多样性指标。【结果】在属水平上,生防菌处理样本与对照样本中的细菌种类显现出明显差异,生防菌处理组Gluconobacter丰度明显升高,放线菌门菌株丰度高于对照组,Burkholderia和Luteibacter等可能与致病相关的菌属丰度大幅下降。刮去外皮后取样,糖丝菌属(Saccharothrix)丰度仍处于较高水平。【结论】Hhs.015能够在苹果树皮内定殖,并影响苹果枝条皮层细菌区系。展开更多
文摘【目的】糖丝菌属(Saccharothrix)是一类丝状稀有放线菌,在生物医药、工业酶制剂和环境修复等领域展现出应用价值。本研究尝试建立以核糖体蛋白质为标志物,利用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)技术鉴定糖丝菌属放线菌的方法。【方法】检索基因组数据库,提取糖丝菌属测序菌株15种核糖体蛋白质的序列并计算理论分子量;通过分子量比对分析糖丝菌属不同菌种之间及其模式菌株与邻近属菌种模式菌株之间15种核糖体蛋白质的匹配度,提出鉴定至菌种及属的核糖体蛋白质匹配数标准;选取目标属和非目标属菌种进行MALDI-TOF MS测试和分析并修正鉴定标准。【结果】将待测菌株的MALDI-TOF质谱峰与糖丝菌属各菌种模式菌株的15种核糖体蛋白质分别匹配,通过最大匹配数、质谱峰强度模式及特征质谱峰鉴定至属或种。【结论】本研究建立了基于15种核糖体蛋白质标志物及MALDI-TOF MS技术鉴定糖丝菌属放线菌的方法,可用于定向筛选和快速鉴定糖丝菌。
基金supported by the National Natural Science Foundation of China(Grant No.2017734)the Knowledge Innovation Program(Grant No.KSCX2-SW-113,KZCX1-SW-12-Ⅱ-02)of CAS
文摘Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsmorphology, physiological and biochemical properties, cellwall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 wassequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was namedSaccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilatedpyrene for growth and 0.005% of yeast extract stimulatedpyrene degradation and assimilation. The optimal pH ofcultural medium and the optimal shaking frequency during cultivation were 6—8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacteriumsp. PYR-1.
文摘【目的】用生防菌Hhs.015发酵液处理苹果发病枝条,研究其对树体微生态的影响。【方法】采用改良CTAB法提取样本中的总DNA,对细菌群落的16S r DNA的V4+V5区进行高通量测序,研究发病枝条涂抹Hhs.015菌悬液30 d后树皮内细菌区系组成变化,分析其相对丰度以及多样性指标。【结果】在属水平上,生防菌处理样本与对照样本中的细菌种类显现出明显差异,生防菌处理组Gluconobacter丰度明显升高,放线菌门菌株丰度高于对照组,Burkholderia和Luteibacter等可能与致病相关的菌属丰度大幅下降。刮去外皮后取样,糖丝菌属(Saccharothrix)丰度仍处于较高水平。【结论】Hhs.015能够在苹果树皮内定殖,并影响苹果枝条皮层细菌区系。
