Background: The late detection of endometrial carcinoma (EC) at an advanced stage often results in a poorpatient prognosis. It is hence important to identify reliable biomarkers to facilitate early detection of EC. Si...Background: The late detection of endometrial carcinoma (EC) at an advanced stage often results in a poorpatient prognosis. It is hence important to identify reliable biomarkers to facilitate early detection of EC. Signaltransducer and activator of transcription (STAT) family members play an important role in several tumors, however,their impact on EC development and progression remains unclear. Methods: Machine learning methods were used toinvestigate the importance of STAT5B in EC. Results: Hence, we explored the UALCAN data mining platform andfound that while STAT1 and STAT2 were upregulated, STAT5A, STAT5B, and STAT6 were downregulated in EC.This high expression of STAT5B and STAT6 predicted favorable clinical outcomes, whereas the increased expressionof STAT1 and STAT2 predicted poor clinical outcomes. Subsequent pathway enrichment analysis revealed that theSTAT family was mainly involved in apoptosis pathway activation, cell cycle disruption, and epithelial–mesenchymaltransition. Drug sensitivity analysis demonstrated that STAT5A/5B expression was negatively correlated with drugresistance in EC. Further, the expression of STAT5B mRNA and protein was correlated with severalclinicopathological characteristics. Tumor Immune Estimation Resource (TIMER) analysis revealed that STAT5Bexpression was positively correlated with the abundance of infiltrating CD8+ T cells and neutrophils while its copynumber variation was associated with the overall immune cell infiltration. The data on the correlations betweenSTAT5B expression and related genes in uterine corpus endometrial carcinoma (UCEC) in cBio Cancer Portalshowed the closest correlation of STAT5B expression with that of KIAA0753 (also known as moonraker and OFIP),followed by COL27A1 in EC. Pathway enrichment analysis further showed that STAT5B-related genes were involvedin the mitogen-activated protein kinase (MAPK) and Ras signaling pathways. Conclusion: Collectively, our findingsprovided new insights into the role of the STAT family in EC. It also highlighted展开更多
信号转导和转录激活因子5b(signal transducer and activator of transcription5b,STAT5b)是JAK-STAT途径中重要的一种转录因子.该转录因子与肿瘤的发生发展密切相关,正日益受到人们的关注.在本研究中,优化STAT5b mRNA序列并合成出一种...信号转导和转录激活因子5b(signal transducer and activator of transcription5b,STAT5b)是JAK-STAT途径中重要的一种转录因子.该转录因子与肿瘤的发生发展密切相关,正日益受到人们的关注.在本研究中,优化STAT5b mRNA序列并合成出一种新型STAT5b金纳米荧光分子信标.该信标由发卡型STAT5b mRNA特异性序列、FITC荧光染料、金纳米粒三部分组成.通过激光共聚焦和流式细胞术等实验方法,实现该信标对HepG-2细胞和PC12细胞中STAT5b mRNA的实时检测.最后,运用RT-PCR方法,该分子信标检测STAT5b mRNA的可靠性得到验证.基于本研究实验结果,可推断该新型金纳米荧光分子信标有望进行JAK-STAT信号通路中其他转录因子的检测.展开更多
文摘Background: The late detection of endometrial carcinoma (EC) at an advanced stage often results in a poorpatient prognosis. It is hence important to identify reliable biomarkers to facilitate early detection of EC. Signaltransducer and activator of transcription (STAT) family members play an important role in several tumors, however,their impact on EC development and progression remains unclear. Methods: Machine learning methods were used toinvestigate the importance of STAT5B in EC. Results: Hence, we explored the UALCAN data mining platform andfound that while STAT1 and STAT2 were upregulated, STAT5A, STAT5B, and STAT6 were downregulated in EC.This high expression of STAT5B and STAT6 predicted favorable clinical outcomes, whereas the increased expressionof STAT1 and STAT2 predicted poor clinical outcomes. Subsequent pathway enrichment analysis revealed that theSTAT family was mainly involved in apoptosis pathway activation, cell cycle disruption, and epithelial–mesenchymaltransition. Drug sensitivity analysis demonstrated that STAT5A/5B expression was negatively correlated with drugresistance in EC. Further, the expression of STAT5B mRNA and protein was correlated with severalclinicopathological characteristics. Tumor Immune Estimation Resource (TIMER) analysis revealed that STAT5Bexpression was positively correlated with the abundance of infiltrating CD8+ T cells and neutrophils while its copynumber variation was associated with the overall immune cell infiltration. The data on the correlations betweenSTAT5B expression and related genes in uterine corpus endometrial carcinoma (UCEC) in cBio Cancer Portalshowed the closest correlation of STAT5B expression with that of KIAA0753 (also known as moonraker and OFIP),followed by COL27A1 in EC. Pathway enrichment analysis further showed that STAT5B-related genes were involvedin the mitogen-activated protein kinase (MAPK) and Ras signaling pathways. Conclusion: Collectively, our findingsprovided new insights into the role of the STAT family in EC. It also highlighted
文摘信号转导和转录激活因子5b(signal transducer and activator of transcription5b,STAT5b)是JAK-STAT途径中重要的一种转录因子.该转录因子与肿瘤的发生发展密切相关,正日益受到人们的关注.在本研究中,优化STAT5b mRNA序列并合成出一种新型STAT5b金纳米荧光分子信标.该信标由发卡型STAT5b mRNA特异性序列、FITC荧光染料、金纳米粒三部分组成.通过激光共聚焦和流式细胞术等实验方法,实现该信标对HepG-2细胞和PC12细胞中STAT5b mRNA的实时检测.最后,运用RT-PCR方法,该分子信标检测STAT5b mRNA的可靠性得到验证.基于本研究实验结果,可推断该新型金纳米荧光分子信标有望进行JAK-STAT信号通路中其他转录因子的检测.
