文章基于赝高电子迁移率晶体管(Pseudomorphic High Electron Mobility Transistor,PHEMT)工艺,设计一款Ka波段单刀四掷反射式开关芯片。为降低芯片插入损耗和提高隔离度,电路选取并联型反射式拓扑结构。同时,为提高工作带宽和减小芯片...文章基于赝高电子迁移率晶体管(Pseudomorphic High Electron Mobility Transistor,PHEMT)工艺,设计一款Ka波段单刀四掷反射式开关芯片。为降低芯片插入损耗和提高隔离度,电路选取并联型反射式拓扑结构。同时,为提高工作带宽和减小芯片面积,采用高低阻抗变换线替代50Ω传输线方式匹配阻抗。芯片采用0 V和-5 V电压控制支路开关的导通或关断。芯片尺寸为1.85 mm×1.55 mm。实测结果表明,在28~42 GHz工作频带范围内,输入输出回波损耗小于-10 dB,插入损耗小于3.2 dB,隔离度大于38 dB,实现了开关芯片低插损、高隔离度的优异性能。展开更多
Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodon...Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.展开更多
目的探讨选择性环氧合酶(COX)-2抑制剂celecoxib影响结肠癌HT-29细胞血管内皮生长因子(VEGF)表达的机制。方法体外培养结肠癌HT-29细胞,采用MTT法检测celecoxib对HT-29细胞增殖的影响。同时针对Sp1和Sp4进行RNA干扰,应用RT-PCR和Wester...目的探讨选择性环氧合酶(COX)-2抑制剂celecoxib影响结肠癌HT-29细胞血管内皮生长因子(VEGF)表达的机制。方法体外培养结肠癌HT-29细胞,采用MTT法检测celecoxib对HT-29细胞增殖的影响。同时针对Sp1和Sp4进行RNA干扰,应用RT-PCR和Western印迹法联合检测celecoxib组和干扰组对HT-29细胞Sp1、Sp4和VEGF m RNA和蛋白表达的影响。结果随着药物浓度的增加(5、10、20、40和80μmol/L)和作用时间的延长(2、4、8、16和32 h),celecoxib对HT-29细胞的增殖具有显著抑制作用,在2、4、8、16和32 h时的半数抑制浓度(IC50)分别为40.36、33.15、31.67、30.94和31.54μmol/L。RT-PCR和Western印迹检测显示,celecoxib可显著降低HT-29细胞Sp1、Sp4和VEGF m RNA和蛋白的表达水平,同时经RNA干扰Sp1(Sp4)后,HT-29细胞Sp1(Sp4)和VEGF m RNA和蛋白的表达水平明显降低,但Sp4(Sp1)m RNA和蛋白表达变化不明显。结论 celecoxib可抑制结肠癌HT-29细胞增殖,其作用机制可能是通过下调Sp1和Sp4表达水平而降低结肠癌细胞中VEGF的表达水平。展开更多
A hydrochemical and tracer study （uranine injection） was conducted in Jurassic limestone of the Posht-e-Naz area in the Alborz belt to evaluate hydraulic relations between a large diameter （about 100m） sinkhole an...A hydrochemical and tracer study （uranine injection） was conducted in Jurassic limestone of the Posht-e-Naz area in the Alborz belt to evaluate hydraulic relations between a large diameter （about 100m） sinkhole and springs and aquifer parameters. A main goal of the project was to find out the source of turbidity of the Emarate drinking water supply spring （SP4） in rainy season. Springs discharge were measured and hydrochemical investigation were carried out. The uranine tracer was injected and eight springs, three wells and the Neka River were selected and totally 989 samples in 107 days were collected. Hydrochemical data demonstrated a relative connection between sinkhole （Shl） and spring （SP4）. The results of the tracing by sampling water indicated only a hydraulic connection between Sange- Nou spring （SP8） and injection point, while the charcoal packets analysis revealed tracer exits from spring numbers SP1, SP3, SP4, SP5, SP8, in wells W1 and W2, and in the Neka River. The concentration - time curves of charcoal packets for qualitative analysis and exit tracer for quantitative analysis is also assessed.展开更多
文摘Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.
文摘目的探讨选择性环氧合酶(COX)-2抑制剂celecoxib影响结肠癌HT-29细胞血管内皮生长因子(VEGF)表达的机制。方法体外培养结肠癌HT-29细胞,采用MTT法检测celecoxib对HT-29细胞增殖的影响。同时针对Sp1和Sp4进行RNA干扰,应用RT-PCR和Western印迹法联合检测celecoxib组和干扰组对HT-29细胞Sp1、Sp4和VEGF m RNA和蛋白表达的影响。结果随着药物浓度的增加(5、10、20、40和80μmol/L)和作用时间的延长(2、4、8、16和32 h),celecoxib对HT-29细胞的增殖具有显著抑制作用,在2、4、8、16和32 h时的半数抑制浓度(IC50)分别为40.36、33.15、31.67、30.94和31.54μmol/L。RT-PCR和Western印迹检测显示,celecoxib可显著降低HT-29细胞Sp1、Sp4和VEGF m RNA和蛋白的表达水平,同时经RNA干扰Sp1(Sp4)后,HT-29细胞Sp1(Sp4)和VEGF m RNA和蛋白的表达水平明显降低,但Sp4(Sp1)m RNA和蛋白表达变化不明显。结论 celecoxib可抑制结肠癌HT-29细胞增殖,其作用机制可能是通过下调Sp1和Sp4表达水平而降低结肠癌细胞中VEGF的表达水平。
文摘A hydrochemical and tracer study （uranine injection） was conducted in Jurassic limestone of the Posht-e-Naz area in the Alborz belt to evaluate hydraulic relations between a large diameter （about 100m） sinkhole and springs and aquifer parameters. A main goal of the project was to find out the source of turbidity of the Emarate drinking water supply spring （SP4） in rainy season. Springs discharge were measured and hydrochemical investigation were carried out. The uranine tracer was injected and eight springs, three wells and the Neka River were selected and totally 989 samples in 107 days were collected. Hydrochemical data demonstrated a relative connection between sinkhole （Shl） and spring （SP4）. The results of the tracing by sampling water indicated only a hydraulic connection between Sange- Nou spring （SP8） and injection point, while the charcoal packets analysis revealed tracer exits from spring numbers SP1, SP3, SP4, SP5, SP8, in wells W1 and W2, and in the Neka River. The concentration - time curves of charcoal packets for qualitative analysis and exit tracer for quantitative analysis is also assessed.
