The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also ...The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum(Se BL). C57 BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or Se BL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the m RNA levels of AMPK, PPAR-α and SREBP1 were invested. Se BL inhibited lipid accumulation in hepatocytes;reduced serum AST and ALT levels;improved dyslipidemia;decreased serum FFAs, TC, TG and LDL-C levels. Se BL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. Se BL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as Se BL, Se BL is more effective than BL. Se BL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.展开更多
Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis.This review surveys the role of Se in the environment,plants and animal bodies,and ...Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis.This review surveys the role of Se in the environment,plants and animal bodies,and discusses data on Se biofortification with different sources of supplementation,from inorganic to organic forms,with special focus on Se-enriched yeast(Se-yeast).Although Se-yeast remains one of the main sources of organic Se,other emerging and innovative sources are reviewed,such as Se-enriched insects and Se-nanoparticles and their potential use in animal nutrition.Se-enriched insects are discussed as an option for supplying Se in organic form to livestock diets.Se-nanoparticles are also discussed,as they represent a more biocompatible and less toxic source of inorganic Se for animal organisms,compared to selenite and selenate.We also provide up to date information on the legal framework in the EU,USA,and Canada of Se that is contained in feed additives.From the scientific evidence available in the literature,it can be concluded that among the inorganic forms,sodium selenite is still one of the main options,whereas Se-yeast remains the primary organic form.However,other potential sources such as Se-enriched insects and Se-nanoparticles are being investigated as they could potentially combine a high bioavailability and reduced Se emissions in the environment.展开更多
基金National Natural Science Foundation of China(Nos.81872917 and 81741164)。
文摘The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum(Se BL). C57 BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or Se BL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the m RNA levels of AMPK, PPAR-α and SREBP1 were invested. Se BL inhibited lipid accumulation in hepatocytes;reduced serum AST and ALT levels;improved dyslipidemia;decreased serum FFAs, TC, TG and LDL-C levels. Se BL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. Se BL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as Se BL, Se BL is more effective than BL. Se BL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.
基金"ASSO-Alimentazione Suina SOstenibile”funded by Regione Lombardia“One Health Action Hub:University Task Force for the resilience of territorial ecosystems”,funded by University of Milan-PSR 2021-GSA-Linea 6。
文摘Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis.This review surveys the role of Se in the environment,plants and animal bodies,and discusses data on Se biofortification with different sources of supplementation,from inorganic to organic forms,with special focus on Se-enriched yeast(Se-yeast).Although Se-yeast remains one of the main sources of organic Se,other emerging and innovative sources are reviewed,such as Se-enriched insects and Se-nanoparticles and their potential use in animal nutrition.Se-enriched insects are discussed as an option for supplying Se in organic form to livestock diets.Se-nanoparticles are also discussed,as they represent a more biocompatible and less toxic source of inorganic Se for animal organisms,compared to selenite and selenate.We also provide up to date information on the legal framework in the EU,USA,and Canada of Se that is contained in feed additives.From the scientific evidence available in the literature,it can be concluded that among the inorganic forms,sodium selenite is still one of the main options,whereas Se-yeast remains the primary organic form.However,other potential sources such as Se-enriched insects and Se-nanoparticles are being investigated as they could potentially combine a high bioavailability and reduced Se emissions in the environment.
