Objective:To analyze the biological functions and its clinical significance of let-7b-5p target gene in cholangiocarcinoma utilizing bioinformatics.Methods:The paper focuses on the let-7b-5p target gene,and predicts i...Objective:To analyze the biological functions and its clinical significance of let-7b-5p target gene in cholangiocarcinoma utilizing bioinformatics.Methods:The paper focuses on the let-7b-5p target gene,and predicts its biological functions as well as related signal pathways through GO biological function and KEGG signal pathway enrichment analysis.The STRING database and Cytoscape are used to construct a protein-protein interaction network to screen core genes.Results:The results of GO analysis showed that let-7b-5p target gene was mainly enriched in biological processes such as Small GTPase binding,Rho GTPase binding,and Rac GTPase binding.The results of KEGG analysis showed that let-7b-5p target gene was significantly enriched in key signaling pathways including Focal adhesion and ECM-receptor interaction.Through protein-protein interaction network and module analysis,CXCL8 and SDC2 were screened as the core site.Conclusion:let-7b-5p can participate in the regulation of biological functions of tumor cells in cholangiocarcinoma,suggesting that it may play an important role as a tumor suppressor gene and biomarker in the occurrence and development of cholangiocarcinoma,which provides new ideas for its diagnosis and treatment.展开更多
基金Open project of Yunnan Provincial Organ Transplantation Clinical Medicine Center,2020SYZ-Z-012.
文摘Objective:To analyze the biological functions and its clinical significance of let-7b-5p target gene in cholangiocarcinoma utilizing bioinformatics.Methods:The paper focuses on the let-7b-5p target gene,and predicts its biological functions as well as related signal pathways through GO biological function and KEGG signal pathway enrichment analysis.The STRING database and Cytoscape are used to construct a protein-protein interaction network to screen core genes.Results:The results of GO analysis showed that let-7b-5p target gene was mainly enriched in biological processes such as Small GTPase binding,Rho GTPase binding,and Rac GTPase binding.The results of KEGG analysis showed that let-7b-5p target gene was significantly enriched in key signaling pathways including Focal adhesion and ECM-receptor interaction.Through protein-protein interaction network and module analysis,CXCL8 and SDC2 were screened as the core site.Conclusion:let-7b-5p can participate in the regulation of biological functions of tumor cells in cholangiocarcinoma,suggesting that it may play an important role as a tumor suppressor gene and biomarker in the occurrence and development of cholangiocarcinoma,which provides new ideas for its diagnosis and treatment.
