利用我室建立的原位鼻咽癌裸鼠模型,建立了稳定性较好的鼻咽癌裸鼠血清蛋白质2-DE方法,并比较了原位鼻咽癌裸鼠血清与对照组裸鼠血清蛋白质2-DE图谱之间的差异.与正常裸鼠血清比较,原位鼻咽癌裸鼠模型组血清增加了4个蛋白质点.利用MALDI...利用我室建立的原位鼻咽癌裸鼠模型,建立了稳定性较好的鼻咽癌裸鼠血清蛋白质2-DE方法,并比较了原位鼻咽癌裸鼠血清与对照组裸鼠血清蛋白质2-DE图谱之间的差异.与正常裸鼠血清比较,原位鼻咽癌裸鼠模型组血清增加了4个蛋白质点.利用MALDI-TOF质谱技术对血清差异蛋白点进行鉴定,发现SAA-1前体(serumamyloidA-1 protein precursor)在鼻咽癌裸鼠模型组血清中明显高表达,为寻找鼻咽癌血清生物标志物提供了有利的线索.展开更多
Objective:Through the bioinformatic analysis of gene chips related to advanced diabetic nephropathy in the GEO database,the key genes of advanced diabetic nephropathy are screened,whose biological functions and signal...Objective:Through the bioinformatic analysis of gene chips related to advanced diabetic nephropathy in the GEO database,the key genes of advanced diabetic nephropathy are screened,whose biological functions and signal pathways are predicted as well.Methods:The gene chips related to advanced diabetic nephropathy from the GEO expression profile database was downloaded,and the differentially expressed genes in patients with advanced diabetic nephropathy and normal people were analyzed through R.For the screened differentially expressed genes,the biological function of GO and the enrichment analysis of KEGG signal pathway were used to predict their biological functions and related signal pathways.In addition,a protein-protein interaction network was constructed,so as to screen core pathogenic genes utilizing STRING database and Cytoscape.Results:By analyzing the chip GSE142025,301 differential genes were obtained,including 197 up-regulated genes and 104 down-regulated genes.Both GO annotation and enrichment analysis suggested that differential genes were mainly involved in immune-inflammatory response and cytokine action.Furthermore,KEGG pathway analysis suggested that the most important pathway related to advanced diabetic nephropathy was MAPK signaling pathway.Through protein-protein interaction network and module analysis,C3,CCR2,CCL19,and SAA1 were selected as the core sites of the interaction.Conclusions:Differential genes participate in the pathogenesis of advanced diabetic nephropathy through the KEGG pathway,the immune inflammatory response and cytokine action,which provides new ways for the diagnosis and treatment of advanced diabetic nephropathy.展开更多
Objective:To explore the value of serum amyloid A content in the diagnosis of gastric cancer in patients with gastric cancer.Methods:The serum of 120 gastric cancer patients and 30 healthy physical examination patient...Objective:To explore the value of serum amyloid A content in the diagnosis of gastric cancer in patients with gastric cancer.Methods:The serum of 120 gastric cancer patients and 30 healthy physical examination patients admitted to Hainan Provincial Hospital of Traditional Chinese Medicine from September 2015 to December 2017 were selected as experimental subjects.Results:The expression of SAA1 in serum of patients with gastric cancer was significantly higher than healthy subjects(42.38±10.12)μg/mL vs.(22.70±8.98)μg/mL,and the difference was statistically significant(t=-9.725,P<0.001).ROC curve analysis showed that the AUC of SAA1 diagnosis of gastric cancer was 0.907(95%CI:0.838-0.976),the detection threshold was 29.02μg/mL,and the diagnostic sensitivity and specificity were 93.3%and 86.7%,respectively.The expression level of serum SAA1 was not correlated with gender(r=0.056,P>0.05),age(r=-0.032,P>0.05)and tumor metastasis(r=-0.173,P>0.05)of gastric cancer patients,and correlated with tumor size(r=-0.228,P<0.05)and invasion degree(r=-0.409,P<0.05),and increased with the increase of tumor invasion degree.Conclusion:The expression level of serum SAA1 in gastric cancer patients is significantly increased,which may be a new potential marker for the diagnosis of gastric cancer.展开更多
文摘利用我室建立的原位鼻咽癌裸鼠模型,建立了稳定性较好的鼻咽癌裸鼠血清蛋白质2-DE方法,并比较了原位鼻咽癌裸鼠血清与对照组裸鼠血清蛋白质2-DE图谱之间的差异.与正常裸鼠血清比较,原位鼻咽癌裸鼠模型组血清增加了4个蛋白质点.利用MALDI-TOF质谱技术对血清差异蛋白点进行鉴定,发现SAA-1前体(serumamyloidA-1 protein precursor)在鼻咽癌裸鼠模型组血清中明显高表达,为寻找鼻咽癌血清生物标志物提供了有利的线索.
基金Kunming Medical University Joint Special Fund of Yunnan Provincial Science and Technology Depart-ment,2018FE001(-098)Open project of Yunnan Provincial Organ Transplantation Clinical Medicine Center,2020SYZ-Z-027。
文摘Objective:Through the bioinformatic analysis of gene chips related to advanced diabetic nephropathy in the GEO database,the key genes of advanced diabetic nephropathy are screened,whose biological functions and signal pathways are predicted as well.Methods:The gene chips related to advanced diabetic nephropathy from the GEO expression profile database was downloaded,and the differentially expressed genes in patients with advanced diabetic nephropathy and normal people were analyzed through R.For the screened differentially expressed genes,the biological function of GO and the enrichment analysis of KEGG signal pathway were used to predict their biological functions and related signal pathways.In addition,a protein-protein interaction network was constructed,so as to screen core pathogenic genes utilizing STRING database and Cytoscape.Results:By analyzing the chip GSE142025,301 differential genes were obtained,including 197 up-regulated genes and 104 down-regulated genes.Both GO annotation and enrichment analysis suggested that differential genes were mainly involved in immune-inflammatory response and cytokine action.Furthermore,KEGG pathway analysis suggested that the most important pathway related to advanced diabetic nephropathy was MAPK signaling pathway.Through protein-protein interaction network and module analysis,C3,CCR2,CCL19,and SAA1 were selected as the core sites of the interaction.Conclusions:Differential genes participate in the pathogenesis of advanced diabetic nephropathy through the KEGG pathway,the immune inflammatory response and cytokine action,which provides new ways for the diagnosis and treatment of advanced diabetic nephropathy.
基金This study was supported by General program of Hainan Natural Science Foundation(818MS159).
文摘Objective:To explore the value of serum amyloid A content in the diagnosis of gastric cancer in patients with gastric cancer.Methods:The serum of 120 gastric cancer patients and 30 healthy physical examination patients admitted to Hainan Provincial Hospital of Traditional Chinese Medicine from September 2015 to December 2017 were selected as experimental subjects.Results:The expression of SAA1 in serum of patients with gastric cancer was significantly higher than healthy subjects(42.38±10.12)μg/mL vs.(22.70±8.98)μg/mL,and the difference was statistically significant(t=-9.725,P<0.001).ROC curve analysis showed that the AUC of SAA1 diagnosis of gastric cancer was 0.907(95%CI:0.838-0.976),the detection threshold was 29.02μg/mL,and the diagnostic sensitivity and specificity were 93.3%and 86.7%,respectively.The expression level of serum SAA1 was not correlated with gender(r=0.056,P>0.05),age(r=-0.032,P>0.05)and tumor metastasis(r=-0.173,P>0.05)of gastric cancer patients,and correlated with tumor size(r=-0.228,P<0.05)and invasion degree(r=-0.409,P<0.05),and increased with the increase of tumor invasion degree.Conclusion:The expression level of serum SAA1 in gastric cancer patients is significantly increased,which may be a new potential marker for the diagnosis of gastric cancer.
