DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for the...DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae.To evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were assessed.We found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample tests.The ITS2 locus had the highest identification efficiency among all tested regions.In a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related species.Therefore,we propose that ITS2 is a promising candidate barcode for plant species identification.展开更多
目的研究芸香科柑橘属植物川佛手Citrusmedica var. sarcodactylis的化学成分,为该药材的开发利用和质量评价提供依据。方法采用各种色谱技术进行分离纯化,通过理化常数测定和波谱分析进行。结果从川佛手中又分离得到7个化合物,其结构...目的研究芸香科柑橘属植物川佛手Citrusmedica var. sarcodactylis的化学成分,为该药材的开发利用和质量评价提供依据。方法采用各种色谱技术进行分离纯化,通过理化常数测定和波谱分析进行。结果从川佛手中又分离得到7个化合物,其结构鉴定为:5-羟基-7-甲氧基-8-异戊烯基香豆素(sibiricol,Ⅵ)、6-羟基-7-甲氧基香豆素(7-methylesculetin,Ⅶ)、佛手柑内酯(bergapten,Ⅷ)、Sigmasteryl acetate(Ⅸ)、5-甲氧基-糠醛(5-methoxyfurfural,Ⅹ)、柠檬苦素(limonin,Ⅺ)、胡萝卜苷(daucosterol,Ⅻ)。结论化合物Ⅵ、Ⅶ、Ⅸ为从芸香科植物中首次分离得到。展开更多
从芸香科植物飞龙掌血中提取分离出4个新三萜酸,经波谱数据分析,分别鉴定为2α,3α,19αtrihydroxy11oxo urs12en28oicacid(1),2α,3α,11α,19αtetrahydroxy urs12en28oicacid(2),2α,3αdihydroxy19oxo18,19sec o urs11,13(18)diene2...从芸香科植物飞龙掌血中提取分离出4个新三萜酸,经波谱数据分析,分别鉴定为2α,3α,19αtrihydroxy11oxo urs12en28oicacid(1),2α,3α,11α,19αtetrahydroxy urs12en28oicacid(2),2α,3αdihydroxy19oxo18,19sec o urs11,13(18)diene28oicacid(3)和2α,3β,19αtrihydroxy olean11,13(18)dien28oicacid(4)。还分离鉴定出已知成分野鸭春酸(5)、arjunicacid(6)、飞龙掌血素、勒钩内脂和β谷甾醇。展开更多
Two new indole alkaloids. wuchuyuamide I and II were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth and their structures were elucidated on the basis of spectral data.
基金supported by the National International Cooperation Program (Grant No. 2007DFA30990)Special Scientific Research Project of Health Profession (Grant No. 200802043)
文摘DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae.To evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were assessed.We found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample tests.The ITS2 locus had the highest identification efficiency among all tested regions.In a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related species.Therefore,we propose that ITS2 is a promising candidate barcode for plant species identification.
文摘目的研究芸香科柑橘属植物川佛手Citrusmedica var. sarcodactylis的化学成分,为该药材的开发利用和质量评价提供依据。方法采用各种色谱技术进行分离纯化,通过理化常数测定和波谱分析进行。结果从川佛手中又分离得到7个化合物,其结构鉴定为:5-羟基-7-甲氧基-8-异戊烯基香豆素(sibiricol,Ⅵ)、6-羟基-7-甲氧基香豆素(7-methylesculetin,Ⅶ)、佛手柑内酯(bergapten,Ⅷ)、Sigmasteryl acetate(Ⅸ)、5-甲氧基-糠醛(5-methoxyfurfural,Ⅹ)、柠檬苦素(limonin,Ⅺ)、胡萝卜苷(daucosterol,Ⅻ)。结论化合物Ⅵ、Ⅶ、Ⅸ为从芸香科植物中首次分离得到。
文摘Two new indole alkaloids. wuchuyuamide I and II were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth and their structures were elucidated on the basis of spectral data.
