In one of our previous papers,we provided general,effective Higgs interactions for the lightest Higgs boson h（SM-like） and a heavier neutral Higgs boson H based on the effective Lagrangian formulation up to the dim-...In one of our previous papers,we provided general,effective Higgs interactions for the lightest Higgs boson h（SM-like） and a heavier neutral Higgs boson H based on the effective Lagrangian formulation up to the dim-6 interactions,and then proposed two sensitive processes for probing H.We showed in several examples that the resonance peak of H and its dim-6 effective coupling constants（ECC） can be detected at LHC Run 2 with reasonable integrated luminosity.In this paper,we further perform a more thorough study of the most sensitive process,pp →VH^＊→VVV,providing information about the relations between the 1σ,3σ,5σ statistical significance and the corresponding ranges of the Higgs ECC for an integrated luminosity of 100 fb^-1.These results have two useful applications in LHC Run 2：（A） realizing the experimental determination of the ECC in the dim-6 interactions if H is found and,（B） obtaining the theoretical exclusion bounds if H is not found.Some alternative processes sensitive for certain ranges of the ECC are also analyzed.展开更多
Objective:This study aimed to explore the expression profile of transcription factor Runt-related transcription factor 2(RUNX2)in the synovial tissues of rheumatoid arthritis(RA)and its effect on the apoptosis of RA f...Objective:This study aimed to explore the expression profile of transcription factor Runt-related transcription factor 2(RUNX2)in the synovial tissues of rheumatoid arthritis(RA)and its effect on the apoptosis of RA fibroblast-like synovial cells(RA-FLS),and to screen new targets for the diagnosis,treatment and prognosis of RA.Methods:The expression and localization of mRNA and protein of RUNX2 in the synovial tissues were detected by real-time quantitative PCR(q PCR)and immunohistochemical staining,respectively.The effect of the expression of exogenous RUNX2 on the apoptosis process of RA-FLS cell line MH7A was investigated by flow cytometry,and the activation of NF-κB signaling pathway was detected by western blotting.Results:The expression of RUNX2 mRNA was significantly higher in the synovial tissues from RA patients,compared to that in the OA patients(P<0.05).RUNX2 protein was mainly localized in the nuclear of the RA synovial cells.Overexpression of exogenous RUNX2 could notably decrease the apoptosis of RA-FLS,which was substantially reversed by pretreatment with SN50,a specific inhibitor of NF-κB pathway.Compared with blank group and negative control group(p CMV6-AC-GFP-vector),the apoptotic rate of exogenous RUNX2 overexpressing(pCMV6-AC-GFP-RUNX2)MH7A cells was significantly decreased(P<0.05).NF-κB signaling pathway activity was significantly increased(P<0.05),and this effect could be effectively reversed by NF-κB signal pathway-specific inhibitor SN5.Conclusion:The high expression of UNX2 in RA synoviocytes could significantly inhibit the spontaneous apoptosis of cells,and it was related to the abnormal activation of NF-κB signaling pathway.In-depth studies of RUNX2/NF-κB signaling pathways will help to identify novel therapeutic targets for RA.展开更多
基金Supported by National Natural Science Foundation of China(11135003 and 11275102)
文摘In one of our previous papers,we provided general,effective Higgs interactions for the lightest Higgs boson h（SM-like） and a heavier neutral Higgs boson H based on the effective Lagrangian formulation up to the dim-6 interactions,and then proposed two sensitive processes for probing H.We showed in several examples that the resonance peak of H and its dim-6 effective coupling constants（ECC） can be detected at LHC Run 2 with reasonable integrated luminosity.In this paper,we further perform a more thorough study of the most sensitive process,pp →VH^＊→VVV,providing information about the relations between the 1σ,3σ,5σ statistical significance and the corresponding ranges of the Higgs ECC for an integrated luminosity of 100 fb^-1.These results have two useful applications in LHC Run 2：（A） realizing the experimental determination of the ECC in the dim-6 interactions if H is found and,（B） obtaining the theoretical exclusion bounds if H is not found.Some alternative processes sensitive for certain ranges of the ECC are also analyzed.
基金supported by Science and Technology Research Project in Shaanxi province(No.2014K11-03-06-10)
文摘Objective:This study aimed to explore the expression profile of transcription factor Runt-related transcription factor 2(RUNX2)in the synovial tissues of rheumatoid arthritis(RA)and its effect on the apoptosis of RA fibroblast-like synovial cells(RA-FLS),and to screen new targets for the diagnosis,treatment and prognosis of RA.Methods:The expression and localization of mRNA and protein of RUNX2 in the synovial tissues were detected by real-time quantitative PCR(q PCR)and immunohistochemical staining,respectively.The effect of the expression of exogenous RUNX2 on the apoptosis process of RA-FLS cell line MH7A was investigated by flow cytometry,and the activation of NF-κB signaling pathway was detected by western blotting.Results:The expression of RUNX2 mRNA was significantly higher in the synovial tissues from RA patients,compared to that in the OA patients(P<0.05).RUNX2 protein was mainly localized in the nuclear of the RA synovial cells.Overexpression of exogenous RUNX2 could notably decrease the apoptosis of RA-FLS,which was substantially reversed by pretreatment with SN50,a specific inhibitor of NF-κB pathway.Compared with blank group and negative control group(p CMV6-AC-GFP-vector),the apoptotic rate of exogenous RUNX2 overexpressing(pCMV6-AC-GFP-RUNX2)MH7A cells was significantly decreased(P<0.05).NF-κB signaling pathway activity was significantly increased(P<0.05),and this effect could be effectively reversed by NF-κB signal pathway-specific inhibitor SN5.Conclusion:The high expression of UNX2 in RA synoviocytes could significantly inhibit the spontaneous apoptosis of cells,and it was related to the abnormal activation of NF-κB signaling pathway.In-depth studies of RUNX2/NF-κB signaling pathways will help to identify novel therapeutic targets for RA.
