[Objectives] To determine the aromatic components of Rosa davurica Pall. [Methods] 42 kinds of aromatic components were identified from the flowers of R. davurica by headspace solid phase microextraction( HS-SPME) com...[Objectives] To determine the aromatic components of Rosa davurica Pall. [Methods] 42 kinds of aromatic components were identified from the flowers of R. davurica by headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS). The main compounds were alcohols( 54. 88%) and aldehydes( 19. 55%). [Results] The top five components with the highest relative content were phenylethyl alcohol( 12. 69%),geraniol( 9. 85%),citronellol( 8. 80%),nerol( 7. 84%) and 2-n-pentylfuran( 7. 45%). [Conclusions] Headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS) can provide basis for further development and utilization of R. davurica.展开更多
采用高效液相色谱法,建立了刺玫果提取物中没食子酸的含量测定方法。色谱条件为：伊利特C18色谱柱（4.6mm×250 mm,5μm）;流动相,V（甲醇）︰V（水）︰V（冰醋酸）（1︰99︰0.3）;流速1 m L/min;进样量20μL;检测波长270 nm;柱温3...采用高效液相色谱法,建立了刺玫果提取物中没食子酸的含量测定方法。色谱条件为：伊利特C18色谱柱（4.6mm×250 mm,5μm）;流动相,V（甲醇）︰V（水）︰V（冰醋酸）（1︰99︰0.3）;流速1 m L/min;进样量20μL;检测波长270 nm;柱温30℃。没食子酸在0.02~1.00μg（r=0.999 5）线性关系良好;没食子酸平均回收率98.40%,RSD为0.54%（n=9）。建立的高效液相色谱含量测定方法具有良好的精密度,结果准确可靠,可用于刺玫果提取物中没食子酸的含量测定。展开更多
基金Supported by Key Science and Technology Project of Gansu Province(1302NKDA028)Science and Technology Planning Project of Lanzhou(2010-1-239+2 种基金 2016-3-4)Talent Project of Lanzhou Science and Technology Bureau(2015-RC-87)Project of Science and Technology Cooperation between Gansu Academy of Agricultural Sciences and Local Areas(2017GAAS63)
文摘[Objectives] To determine the aromatic components of Rosa davurica Pall. [Methods] 42 kinds of aromatic components were identified from the flowers of R. davurica by headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS). The main compounds were alcohols( 54. 88%) and aldehydes( 19. 55%). [Results] The top five components with the highest relative content were phenylethyl alcohol( 12. 69%),geraniol( 9. 85%),citronellol( 8. 80%),nerol( 7. 84%) and 2-n-pentylfuran( 7. 45%). [Conclusions] Headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS) can provide basis for further development and utilization of R. davurica.
