In this study, 5 parental rice varieties with different allelopathic potentials were employed in diallel cross [P(P+1)/2] to get a set of genetic materials including parental lines and two generations of F1s. The dyna...In this study, 5 parental rice varieties with different allelopathic potentials were employed in diallel cross [P(P+1)/2] to get a set of genetic materials including parental lines and two generations of F1s. The dynamic heterosis for allelopathy in rice under different environmental conditions, was analyzed by using additive-dominant developmental genetic model. The results indicated that heterosis in both F1 and F2 showed inhibitory effects on shoot and root length of receiver plant(Lactuca sativa L.). Heterosis over mid-parent based on population mean(HMP)in F2 was lower than that in differental environmental conditions, showing 1/2 HMP in F1 The heterosis in rice allelopathy was much higher under the field environmental conditions with lower temperature and weaker sunlight than that under favorable environment, implying that the allel-opthic potential could be increased by stress environment. This finding interpreted the genetic reason that plant could produce more allelochemicals under unfavorable environment.展开更多
By using differential display PCR (DD-PCR) technique, two salt-inducible and one salt-repressed cDNA fragments were isolated from rice. The three cDNA fragments were characterized respectively as partial sequence of r...By using differential display PCR (DD-PCR) technique, two salt-inducible and one salt-repressed cDNA fragments were isolated from rice. The three cDNA fragments were characterized respectively as partial sequence of rice S-adenosylmethionine decarboxylase (SAMDC) gene, a new member of translation elongation factor lA gene (named REF1A), and a novel gene whose function is unknown (named SRG1). The full-length cDNA of SAMDC gene (named SAMDC1) was further isolated by RT-PCR approach and the deduced polypeptide was found to be homologous to SAMDC proteins of other plants, yeast and human. Northern hybridization revealed that expression of SAMDC1 and REF1A was induced, while SRG1 was dramatically repressed, by salinity stress. Southern blot analysis demonstrated that SAMDC 1 and SRG1 were present as a single copy gene in rice genome, whereas rice REF1A gene was organized as a gene family. The REF1A, SAMDC1, and SRG1 genes were located on chromosome 3,4, and 6 respectively by RFLP mapping approach using ZYQ8/JX17 DH population and RFLP linkage maps.展开更多
The straw of Grain-Straw-Dual-Use-Rice (GSDUR) variety 201 of which the grain quality and yield were equivalent to that of common rice variety (the grain yield approximately 7.5 t ha-1), but straw protein content was ...The straw of Grain-Straw-Dual-Use-Rice (GSDUR) variety 201 of which the grain quality and yield were equivalent to that of common rice variety (the grain yield approximately 7.5 t ha-1), but straw protein content was 9.31% (common rice straw i. e. CK was approximately 4.0%), and other eight fodder indexes were better than CK to some extent, was employed to feed animals. 15N tracing result suggested that the protein in 201 straw could be effectively transformed into fish body protein and white mouse body protein. The digestibility of fodder, the 15N recovery rate of animal body and the absorption of fodder protein were 13. 8, 9. 6, 24.49% and 16. 5, 6.0, 47. 2% higher than those of common rice straw respectively when feeding grass carp and white mice with 201 rice straw, whereas the 15N recovery rates of animal manure were 3. 25, 6. 5% lower than those of common rice straw, respectively. The results of feeding animals with 201 straw were as follows: (1)The milk yield of cow was increased by 8.3 % as compared to that of common rice straw. 843. 8 kg milk could be produced with 7. 5 t ha-1 of dry 201 straw. (2)Fish daily weight gain, straw consumption of grass carp, fish weight gain per kg fresh rice straw were increased by 60.0, 16.8 and 37.0 % respectively when 201 fresh straw was used to feed grass carp compared to feeding CK, and fish yield could be increased by 297.5 kg with 25.0 t ha-1 of fresh 201 straw. (3)The goose daily weight gain and the ratio of fodder and meat were increased by 33. 9 and 26.8 % respectively when 201 rice straw was used as the main raw material of the compound fodder to feed white geese compared to feeding CK, and geese weight could be increased by 2 358.0 kg with one hectare of 201 straw. (4)Not only were the feeding results identical when high quality 201 rice straw powder substituting for wheat bran which made up of 5 % compound fodder to feed cross bred pigs compared to CK, but 0.11 kg fine fodder could be saved when 1 kg cross bred pig weight was increased.展开更多
基金supported by the National Natural Science Foundation of China(30070068)Provincial Key Scientific and Technological Program(2002F012)Provincial Natural Science Foundation(D0110012)of Fujfan,China.
文摘In this study, 5 parental rice varieties with different allelopathic potentials were employed in diallel cross [P(P+1)/2] to get a set of genetic materials including parental lines and two generations of F1s. The dynamic heterosis for allelopathy in rice under different environmental conditions, was analyzed by using additive-dominant developmental genetic model. The results indicated that heterosis in both F1 and F2 showed inhibitory effects on shoot and root length of receiver plant(Lactuca sativa L.). Heterosis over mid-parent based on population mean(HMP)in F2 was lower than that in differental environmental conditions, showing 1/2 HMP in F1 The heterosis in rice allelopathy was much higher under the field environmental conditions with lower temperature and weaker sunlight than that under favorable environment, implying that the allel-opthic potential could be increased by stress environment. This finding interpreted the genetic reason that plant could produce more allelochemicals under unfavorable environment.
基金Project supported by the National "863" High-Technology Program.
文摘By using differential display PCR (DD-PCR) technique, two salt-inducible and one salt-repressed cDNA fragments were isolated from rice. The three cDNA fragments were characterized respectively as partial sequence of rice S-adenosylmethionine decarboxylase (SAMDC) gene, a new member of translation elongation factor lA gene (named REF1A), and a novel gene whose function is unknown (named SRG1). The full-length cDNA of SAMDC gene (named SAMDC1) was further isolated by RT-PCR approach and the deduced polypeptide was found to be homologous to SAMDC proteins of other plants, yeast and human. Northern hybridization revealed that expression of SAMDC1 and REF1A was induced, while SRG1 was dramatically repressed, by salinity stress. Southern blot analysis demonstrated that SAMDC 1 and SRG1 were present as a single copy gene in rice genome, whereas rice REF1A gene was organized as a gene family. The REF1A, SAMDC1, and SRG1 genes were located on chromosome 3,4, and 6 respectively by RFLP mapping approach using ZYQ8/JX17 DH population and RFLP linkage maps.
文摘The straw of Grain-Straw-Dual-Use-Rice (GSDUR) variety 201 of which the grain quality and yield were equivalent to that of common rice variety (the grain yield approximately 7.5 t ha-1), but straw protein content was 9.31% (common rice straw i. e. CK was approximately 4.0%), and other eight fodder indexes were better than CK to some extent, was employed to feed animals. 15N tracing result suggested that the protein in 201 straw could be effectively transformed into fish body protein and white mouse body protein. The digestibility of fodder, the 15N recovery rate of animal body and the absorption of fodder protein were 13. 8, 9. 6, 24.49% and 16. 5, 6.0, 47. 2% higher than those of common rice straw respectively when feeding grass carp and white mice with 201 rice straw, whereas the 15N recovery rates of animal manure were 3. 25, 6. 5% lower than those of common rice straw, respectively. The results of feeding animals with 201 straw were as follows: (1)The milk yield of cow was increased by 8.3 % as compared to that of common rice straw. 843. 8 kg milk could be produced with 7. 5 t ha-1 of dry 201 straw. (2)Fish daily weight gain, straw consumption of grass carp, fish weight gain per kg fresh rice straw were increased by 60.0, 16.8 and 37.0 % respectively when 201 fresh straw was used to feed grass carp compared to feeding CK, and fish yield could be increased by 297.5 kg with 25.0 t ha-1 of fresh 201 straw. (3)The goose daily weight gain and the ratio of fodder and meat were increased by 33. 9 and 26.8 % respectively when 201 rice straw was used as the main raw material of the compound fodder to feed white geese compared to feeding CK, and geese weight could be increased by 2 358.0 kg with one hectare of 201 straw. (4)Not only were the feeding results identical when high quality 201 rice straw powder substituting for wheat bran which made up of 5 % compound fodder to feed cross bred pigs compared to CK, but 0.11 kg fine fodder could be saved when 1 kg cross bred pig weight was increased.
