【目的】筛选产多糖光合菌,为开发廉价易制备的益生菌多糖及应用提供参考。【方法】从虾塘中筛选一株产多糖的光合菌HP1,进行生理生化鉴定和16S rDNA序列比对。通过醇沉的方式从发酵液中制备光合菌HP1粗多糖,拌喂乌鳢30 d,以黄芪多糖作...【目的】筛选产多糖光合菌,为开发廉价易制备的益生菌多糖及应用提供参考。【方法】从虾塘中筛选一株产多糖的光合菌HP1,进行生理生化鉴定和16S rDNA序列比对。通过醇沉的方式从发酵液中制备光合菌HP1粗多糖,拌喂乌鳢30 d,以黄芪多糖作为参照,分别对比各个试验组对乌鳢肌肉营养成分的影响。【结果】HP1为粪红假单胞菌 Rhodopseudomonasfaecalis 。HP1胞外多糖组对乌鳢肌肉pH值没有显著影响( P >0.05),但能显著降低肌肉失水率并促使肌纤维直径变细( P <0.05)。拌喂HP1胞外多糖对乌鳢粗蛋白含量无显著影响( P >0.05),但对乌鳢肌肉中的氨基酸组成有显著影响( P <0.05),分别提高乌鳢肌肉中的总氨基酸、必需氨基酸、呈味氨基酸含量8.7%、11%、9%。另外,拌喂HP1胞外多糖也分别提高了乌鳢肌肉的粗脂肪含量、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)总含量10.7%和12.8%,显著提高肌肉中n-3与n-6系列脂肪酸比值。【结论】拌喂HP1胞外多糖不仅提高乌鳢肌肉的食用口感,也提升了乌鳢肌肉的营养价值,其效果与黄芪多糖拌喂的效果相似。展开更多
In this work, the optimization of hydrogen production by photo-fermentation bacteria immobilized on agar gel granule was systematic investigated in batch culture. Experiment focus on the effect of some important affec...In this work, the optimization of hydrogen production by photo-fermentation bacteria immobilized on agar gel granule was systematic investigated in batch culture. Experiment focus on the effect of some important affecting factors on photo-hydrogen production. Results indicated that immobilized Rhodopseudomonas faecalis RLD-53 exhibited the highest hydrogen yield of 3.15 mol H2/mol acetate under follow optimal condition: agar granule diameter of 2.5 mm, inoculum age of 24 h, agar concentration of 2%, biomass of 4 mg/ml in agar and light intensity of 9000 lux. More importantly, immobilized photo-fermentation bacteria not only can enhance hydrogen production but can increase acids-tolerance capacity, even at pH 5.0 hydrogen also was produced, and thus hopefully immobilized photo-fermentation bacteria can be applied in the combination of dark and photo-fermentation for hydrogen production with high yield.展开更多
文摘【目的】筛选产多糖光合菌,为开发廉价易制备的益生菌多糖及应用提供参考。【方法】从虾塘中筛选一株产多糖的光合菌HP1,进行生理生化鉴定和16S rDNA序列比对。通过醇沉的方式从发酵液中制备光合菌HP1粗多糖,拌喂乌鳢30 d,以黄芪多糖作为参照,分别对比各个试验组对乌鳢肌肉营养成分的影响。【结果】HP1为粪红假单胞菌 Rhodopseudomonasfaecalis 。HP1胞外多糖组对乌鳢肌肉pH值没有显著影响( P >0.05),但能显著降低肌肉失水率并促使肌纤维直径变细( P <0.05)。拌喂HP1胞外多糖对乌鳢粗蛋白含量无显著影响( P >0.05),但对乌鳢肌肉中的氨基酸组成有显著影响( P <0.05),分别提高乌鳢肌肉中的总氨基酸、必需氨基酸、呈味氨基酸含量8.7%、11%、9%。另外,拌喂HP1胞外多糖也分别提高了乌鳢肌肉的粗脂肪含量、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)总含量10.7%和12.8%,显著提高肌肉中n-3与n-6系列脂肪酸比值。【结论】拌喂HP1胞外多糖不仅提高乌鳢肌肉的食用口感,也提升了乌鳢肌肉的营养价值,其效果与黄芪多糖拌喂的效果相似。
文摘In this work, the optimization of hydrogen production by photo-fermentation bacteria immobilized on agar gel granule was systematic investigated in batch culture. Experiment focus on the effect of some important affecting factors on photo-hydrogen production. Results indicated that immobilized Rhodopseudomonas faecalis RLD-53 exhibited the highest hydrogen yield of 3.15 mol H2/mol acetate under follow optimal condition: agar granule diameter of 2.5 mm, inoculum age of 24 h, agar concentration of 2%, biomass of 4 mg/ml in agar and light intensity of 9000 lux. More importantly, immobilized photo-fermentation bacteria not only can enhance hydrogen production but can increase acids-tolerance capacity, even at pH 5.0 hydrogen also was produced, and thus hopefully immobilized photo-fermentation bacteria can be applied in the combination of dark and photo-fermentation for hydrogen production with high yield.
