The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this spe...The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.展开更多
In most of Arabian courtiers Rhazya stricta is extensively utilized in public medicine for several diseases treatment.In this study,crude alkaloid extract of R.stricta(CAERS)coated with silver nanoparticles(CAERS-AgNP...In most of Arabian courtiers Rhazya stricta is extensively utilized in public medicine for several diseases treatment.In this study,crude alkaloid extract of R.stricta(CAERS)coated with silver nanoparticles(CAERS-AgNPs)as potential treatment against diabetes in DM animal model was evaluated.Swiss albino male rats(n=80)were injected with STZ to induce Diabetes Mellitus type-2(DM).DM-rats were injected different doses of CAERS or CAERS-nanoparticles(CAERS-NPs)for 2 months.The results exhibited that mRNA expression of insulin and insulin receptor was down-regulated,activity levels of antioxidant enzymes were decreased,generation of ROS mediated DNA adducts and apoptosis in DM-rats was increased significantly than those in negative control rats.In contrast,the expression of insulin and insulin receptor genes was up-regulated,activity levels of antioxidant enzymes,ROS generation,DNA adducts and apoptosis incidence in DM-rats supplemented with high dose of CAERS and all doses of CAERS-NPs were improved.In conclusion:R.stricta nanoparticles improved the anti-diabetic effect of the plant much more than the powder form of the extract.This action could be attributed to modification of the chemical and physical properties of the plant materials.The properties modification might be improved the ability of plant compounds to penetrate the cell membrane which facilitating release of plant materials into the target cells.展开更多
文摘The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.
文摘In most of Arabian courtiers Rhazya stricta is extensively utilized in public medicine for several diseases treatment.In this study,crude alkaloid extract of R.stricta(CAERS)coated with silver nanoparticles(CAERS-AgNPs)as potential treatment against diabetes in DM animal model was evaluated.Swiss albino male rats(n=80)were injected with STZ to induce Diabetes Mellitus type-2(DM).DM-rats were injected different doses of CAERS or CAERS-nanoparticles(CAERS-NPs)for 2 months.The results exhibited that mRNA expression of insulin and insulin receptor was down-regulated,activity levels of antioxidant enzymes were decreased,generation of ROS mediated DNA adducts and apoptosis in DM-rats was increased significantly than those in negative control rats.In contrast,the expression of insulin and insulin receptor genes was up-regulated,activity levels of antioxidant enzymes,ROS generation,DNA adducts and apoptosis incidence in DM-rats supplemented with high dose of CAERS and all doses of CAERS-NPs were improved.In conclusion:R.stricta nanoparticles improved the anti-diabetic effect of the plant much more than the powder form of the extract.This action could be attributed to modification of the chemical and physical properties of the plant materials.The properties modification might be improved the ability of plant compounds to penetrate the cell membrane which facilitating release of plant materials into the target cells.
