The administration of nanoparticles(NPs)first faces the challenges of evading renal filtration and clearance of reticuloendothelial system(RES).After that,NPs infiltrate through the expanded endothelial space and pene...The administration of nanoparticles(NPs)first faces the challenges of evading renal filtration and clearance of reticuloendothelial system(RES).After that,NPs infiltrate through the expanded endothelial space and penetrated the dense stroma of tumor microenvironment to tumor cells.As long as possible to prolong the time of NPs remaining in tumor tissue,NPs release active agent and induce pharmacological action.This review provides a comprehensive summary of the physical and chemical properties of NPs and the influence of various biological factors in tumor microenvironment,and discusses how to improve the final efficacy through adjusting the characteristics and structure of NPs.Perspectives and future directions are also provided.展开更多
Objective:observing the effect of "Huaban" granule on REC function and CIC content of mouse with anaphylactoid purpura.Method:Animal model was made by gliadin per.So as antigene and reticuloendothelial syste...Objective:observing the effect of "Huaban" granule on REC function and CIC content of mouse with anaphylactoid purpura.Method:Animal model was made by gliadin per.So as antigene and reticuloendothelial system blocked with india-ink,indexes which reflected RES function such as k value,a value and CIC turbity were determinated respectively.Results:The preventive group of "Huaban" granule could enhance model mouses RES function and lower CIC content,this indicated the importance of early and whole cource administration.Conclusion:The mechanism of "Huaban" granule preventing anaphylactoid purpura maybe lowering CIC content and its local deposit by enhancing model mouses RES function and the produce of CIC antigene or the eliminating of CIC.展开更多
Phage display technique is a powerful approach for discovering new tumor-and organ-targeting ligands,and radiolabeled phage has a potential to analyze the phage-binding sensitivity and specific imaging.In this study,p...Phage display technique is a powerful approach for discovering new tumor-and organ-targeting ligands,and radiolabeled phage has a potential to analyze the phage-binding sensitivity and specific imaging.In this study,phage Ⅱ (the spleen-targeting phage) in mice was isolated after three rounds biopanning,and labeled by 99mTc using mercaptoacetyltriglycine (MAG3) as chelator to evaluate their binding properties in vivo.The amount of phage Ⅱ eluted from spleen was enriched by plague assay each round.99mTc-MAG3-phage Ⅱ showed the less retention in blood at any time point than half that of 99mTc-MAG3-phage Ⅰ (the radiolabeled original Ph.D-12 phage as control).The accumulation in spleen between 99mTc-MAG3-phage Ⅰ and Ⅱ was of different tendency.The highest uptake of 99mTc-MAG3-phage Ⅱ in spleen was 24.80 %ID/g at 30 min;and of 99mTc-MAG3-phage I,30.93% ID/g at 5 min.After circulating 99mTc-MAG3-phage Ⅱ for 120 min,its accumulation in spleen decreased though higher than that of 99mTc-MAG3-phage Ⅰ.In other organs,the 99mTc-MAG3-phage Ⅱ showed low retention and high spleen-to-organ or tissue ratios.In conclusion,the radiolabeled phage Ⅱ is convenient for studying the binding and specificity of spleen-targeting peptides found via phage display in vivo.展开更多
基金the National Natural Science Foundation of China(No.81971729)for financial support
文摘The administration of nanoparticles(NPs)first faces the challenges of evading renal filtration and clearance of reticuloendothelial system(RES).After that,NPs infiltrate through the expanded endothelial space and penetrated the dense stroma of tumor microenvironment to tumor cells.As long as possible to prolong the time of NPs remaining in tumor tissue,NPs release active agent and induce pharmacological action.This review provides a comprehensive summary of the physical and chemical properties of NPs and the influence of various biological factors in tumor microenvironment,and discusses how to improve the final efficacy through adjusting the characteristics and structure of NPs.Perspectives and future directions are also provided.
文摘Objective:observing the effect of "Huaban" granule on REC function and CIC content of mouse with anaphylactoid purpura.Method:Animal model was made by gliadin per.So as antigene and reticuloendothelial system blocked with india-ink,indexes which reflected RES function such as k value,a value and CIC turbity were determinated respectively.Results:The preventive group of "Huaban" granule could enhance model mouses RES function and lower CIC content,this indicated the importance of early and whole cource administration.Conclusion:The mechanism of "Huaban" granule preventing anaphylactoid purpura maybe lowering CIC content and its local deposit by enhancing model mouses RES function and the produce of CIC antigene or the eliminating of CIC.
基金Supported by National Natural Science Foundation of China (No. 20771011 and 21071010)the Ministry of Science and Technology of China (No.2006CB705700)
文摘Phage display technique is a powerful approach for discovering new tumor-and organ-targeting ligands,and radiolabeled phage has a potential to analyze the phage-binding sensitivity and specific imaging.In this study,phage Ⅱ (the spleen-targeting phage) in mice was isolated after three rounds biopanning,and labeled by 99mTc using mercaptoacetyltriglycine (MAG3) as chelator to evaluate their binding properties in vivo.The amount of phage Ⅱ eluted from spleen was enriched by plague assay each round.99mTc-MAG3-phage Ⅱ showed the less retention in blood at any time point than half that of 99mTc-MAG3-phage Ⅰ (the radiolabeled original Ph.D-12 phage as control).The accumulation in spleen between 99mTc-MAG3-phage Ⅰ and Ⅱ was of different tendency.The highest uptake of 99mTc-MAG3-phage Ⅱ in spleen was 24.80 %ID/g at 30 min;and of 99mTc-MAG3-phage I,30.93% ID/g at 5 min.After circulating 99mTc-MAG3-phage Ⅱ for 120 min,its accumulation in spleen decreased though higher than that of 99mTc-MAG3-phage Ⅰ.In other organs,the 99mTc-MAG3-phage Ⅱ showed low retention and high spleen-to-organ or tissue ratios.In conclusion,the radiolabeled phage Ⅱ is convenient for studying the binding and specificity of spleen-targeting peptides found via phage display in vivo.
