以冷榨藤椒油和溶剂萃取的藤椒油为原料,采用体外抗氧化实验研究其抗氧化能力。以BHT和VC为阳性对照,通过测定两种藤椒油还原力、对·OH、DPPH·、O-2·清除率,探讨藤椒油的抗氧化能力。结果表明,冷榨油和溶剂油的还原能力...以冷榨藤椒油和溶剂萃取的藤椒油为原料,采用体外抗氧化实验研究其抗氧化能力。以BHT和VC为阳性对照,通过测定两种藤椒油还原力、对·OH、DPPH·、O-2·清除率,探讨藤椒油的抗氧化能力。结果表明,冷榨油和溶剂油的还原能力随其质量浓度的增加而增强,对·OH、DPPH·、O-2·清除率随质量浓度的增加而增大;溶剂油对·OH、DPPH·的清除率高于同质量浓度的冷榨油;冷榨油对·OH、DPPH·、O-2·的IC50值分别为4.0675、10.3052和0.5415mg/m L;溶剂油对·OH、DPPH·、O-2·的IC50值分别为1.9606、2.9465和1.6053mg/m L。当溶剂油质量浓度达到9mg/m L时,对·OH清除率达到89.79%,其清除效果高于1mg/m L VC对·OH(清除率71.82%)清除效果;当溶剂油质量浓度达到11mg/m L时,对DPPH·清除率达到86.87%,远高于1mg/m L BHT清除率(44.51%)。展开更多
Objective:To determine the antioxidant activity,total phenolic and flavonoid content of Petroleum ether extract（PE）,Dichloromethane extract（DCM）,Ethanol extract（ET） and aqueous extract（AQ） of henna seeds.Metho...Objective:To determine the antioxidant activity,total phenolic and flavonoid content of Petroleum ether extract（PE）,Dichloromethane extract（DCM）,Ethanol extract（ET） and aqueous extract（AQ） of henna seeds.Methods:Total antioxidant assay（phosphomolybenum method）, DPPH radical scavenging assay,reducing power assay and lipid peroxidation inhibition assay were used to ascertain the potential of seeds as an antioxidant.Results:In all the assays carried out ET showed a greater potential to scavenge DPPH radical,reduce MO（Ⅳ） to MO（Ⅴ） complex and Fe（Ⅲ） to Fe（Ⅱ） and to inhibit lipid peroxidation.The IC<sub>50</sub> of ET was far greater than that of the standard,ascorbic acid（AS） in the lipid peroxidation assay.The activity of AQ was lesser when compared with that of ET but greater than PE and DCM.The amount of phenolics and flavonoids were present in higher amounts in ET followed by AQ.Trace amounts of phenolics were detected in PE and DCM,but the amount of flavonoids were below the detection level.The study showed that the antioxidant activity and the concentrations of phenolics and flavonoids are proportionate to each other.Conclusions:Ethanolic extract of henna seeds are efficient antioxidants,which can be utilized for further isolation of active compounds and pharmaceutical applications.展开更多
Objective:To assess the antioxidant activity of Ficus pseudopalma Blanco(Moraceae)(F.pseudopalma) and characterize the active components present in it.Methods:Column chromatography of crude ethanol leaf extract of F.p...Objective:To assess the antioxidant activity of Ficus pseudopalma Blanco(Moraceae)(F.pseudopalma) and characterize the active components present in it.Methods:Column chromatography of crude ethanol leaf extract of F.pseudopalma was performed and seven fractions were obtained,labeled as Ft.F2,F3,F4.F5,F6,F7.DPPH.FRAP,Criess,Kenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions.Thin layer chromatography(TLC),high performance liquid chromatography and Fourier transfer infrared spectroscopy(FTIR) were performed to identify and characterize the bioactive component present in each fractions of F.pseudopalma.Results:DPPH and FRAP assay showed that F5.F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions.All fractions exhibited a good nitric oxide radical scavenging activity wherein F1,F2 and F3 showed the highest inhibition.However,all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals.Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions.Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6.Infrared spectra of the fractions revealed the presence of C-C,OH,aromatic C=C and C=O groups.Conclusions:The identified lupeol in F.pseudopalma may be responsible for the exhibited antioxidant property of the plant.Furthermore,knowing the antioxidant capability of the plant,F.pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases.展开更多
Objective:To evaluate the antioxidant potential of methanolic leaf extract of Indigofera cassioides(MEIC) using various in vilro antioxidant assay systems.Methods:Antioxidant and free radical scavenging activity of ME...Objective:To evaluate the antioxidant potential of methanolic leaf extract of Indigofera cassioides(MEIC) using various in vilro antioxidant assay systems.Methods:Antioxidant and free radical scavenging activity of MEIC was assayed by using different in vitro models like ABTS, DPPH,nitric oxide,superoxide,hydrogen peroxide and hydroxyl radical.Reductive ability of the extract was tested by the complex formation with potassium ferricyanide.Further total phenol and flavonoid contents of the crude extract were also determined.Rutin and ascorbic acid were used as standards.Results:MEIC exhibited potent and concentration dependent free radical scavenging activity in all the tested models.Reductive ability was also found to increase with increase in MEIC concentration.Total phenol and flavonoid content determination showed that the extract is rich in phenols and flavonoids.Conclusions:All the results of the in vitro antioxidant assays reveal potent antioxidant and free radical scavenging activity of the leaves of Indigofera cassioides,equivalent to that of standard ascorbic acid and rutin.This potent antioxidant activity may be attributed to its high phenolic and flavonoid contents.展开更多
文摘以冷榨藤椒油和溶剂萃取的藤椒油为原料,采用体外抗氧化实验研究其抗氧化能力。以BHT和VC为阳性对照,通过测定两种藤椒油还原力、对·OH、DPPH·、O-2·清除率,探讨藤椒油的抗氧化能力。结果表明,冷榨油和溶剂油的还原能力随其质量浓度的增加而增强,对·OH、DPPH·、O-2·清除率随质量浓度的增加而增大;溶剂油对·OH、DPPH·的清除率高于同质量浓度的冷榨油;冷榨油对·OH、DPPH·、O-2·的IC50值分别为4.0675、10.3052和0.5415mg/m L;溶剂油对·OH、DPPH·、O-2·的IC50值分别为1.9606、2.9465和1.6053mg/m L。当溶剂油质量浓度达到9mg/m L时,对·OH清除率达到89.79%,其清除效果高于1mg/m L VC对·OH(清除率71.82%)清除效果;当溶剂油质量浓度达到11mg/m L时,对DPPH·清除率达到86.87%,远高于1mg/m L BHT清除率(44.51%)。
文摘Objective:To determine the antioxidant activity,total phenolic and flavonoid content of Petroleum ether extract（PE）,Dichloromethane extract（DCM）,Ethanol extract（ET） and aqueous extract（AQ） of henna seeds.Methods:Total antioxidant assay（phosphomolybenum method）, DPPH radical scavenging assay,reducing power assay and lipid peroxidation inhibition assay were used to ascertain the potential of seeds as an antioxidant.Results:In all the assays carried out ET showed a greater potential to scavenge DPPH radical,reduce MO（Ⅳ） to MO（Ⅴ） complex and Fe（Ⅲ） to Fe（Ⅱ） and to inhibit lipid peroxidation.The IC<sub>50</sub> of ET was far greater than that of the standard,ascorbic acid（AS） in the lipid peroxidation assay.The activity of AQ was lesser when compared with that of ET but greater than PE and DCM.The amount of phenolics and flavonoids were present in higher amounts in ET followed by AQ.Trace amounts of phenolics were detected in PE and DCM,but the amount of flavonoids were below the detection level.The study showed that the antioxidant activity and the concentrations of phenolics and flavonoids are proportionate to each other.Conclusions:Ethanolic extract of henna seeds are efficient antioxidants,which can be utilized for further isolation of active compounds and pharmaceutical applications.
基金Supported by Grants-in-aid of the Philippine Council for Health Research and Development,Department of Science and Technology(Grant no.120023)
文摘Objective:To assess the antioxidant activity of Ficus pseudopalma Blanco(Moraceae)(F.pseudopalma) and characterize the active components present in it.Methods:Column chromatography of crude ethanol leaf extract of F.pseudopalma was performed and seven fractions were obtained,labeled as Ft.F2,F3,F4.F5,F6,F7.DPPH.FRAP,Criess,Kenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions.Thin layer chromatography(TLC),high performance liquid chromatography and Fourier transfer infrared spectroscopy(FTIR) were performed to identify and characterize the bioactive component present in each fractions of F.pseudopalma.Results:DPPH and FRAP assay showed that F5.F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions.All fractions exhibited a good nitric oxide radical scavenging activity wherein F1,F2 and F3 showed the highest inhibition.However,all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals.Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions.Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6.Infrared spectra of the fractions revealed the presence of C-C,OH,aromatic C=C and C=O groups.Conclusions:The identified lupeol in F.pseudopalma may be responsible for the exhibited antioxidant property of the plant.Furthermore,knowing the antioxidant capability of the plant,F.pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases.
文摘Objective:To evaluate the antioxidant potential of methanolic leaf extract of Indigofera cassioides(MEIC) using various in vilro antioxidant assay systems.Methods:Antioxidant and free radical scavenging activity of MEIC was assayed by using different in vitro models like ABTS, DPPH,nitric oxide,superoxide,hydrogen peroxide and hydroxyl radical.Reductive ability of the extract was tested by the complex formation with potassium ferricyanide.Further total phenol and flavonoid contents of the crude extract were also determined.Rutin and ascorbic acid were used as standards.Results:MEIC exhibited potent and concentration dependent free radical scavenging activity in all the tested models.Reductive ability was also found to increase with increase in MEIC concentration.Total phenol and flavonoid content determination showed that the extract is rich in phenols and flavonoids.Conclusions:All the results of the in vitro antioxidant assays reveal potent antioxidant and free radical scavenging activity of the leaves of Indigofera cassioides,equivalent to that of standard ascorbic acid and rutin.This potent antioxidant activity may be attributed to its high phenolic and flavonoid contents.
