In this study, we developed a systemic PD model in middle-aged cynomolgus monkeys using individualized low-dose MPTP, to explore effective indicators for the early prediction of clinical outcomes. MPTP was not stopped...In this study, we developed a systemic PD model in middle-aged cynomolgus monkeys using individualized low-dose MPTP, to explore effective indicators for the early prediction of clinical outcomes. MPTP was not stopped until the animals showed typical PD motor symptoms on days 10 to 13 after MPTP administration when the Kurlan score reached 10; this abrogated the dif- ferences in individual susceptibility to MPTP. The clinical symptoms persisted, peaking on days 3 to 12 after MPTP withdrawal （rapid progress stage）, and then the Kurlan score plateaued. A Kurlan score at the end of the rapid progress stage 〉15 reflected stable or slowly-progressive PD, while a score 〈15 indicated spontaneous recovery. The entire clinical evolution and outcome of the systemic PD model was characterized in this study, thus providing options for therapeutic and translational research.展开更多
BACKGROUND Along with the unceasing progress of medicine,Crohn's disease(CD),especially complex CD,is no longer a taboo for minimally invasive surgery.However,considering its special disease characteristics,more c...BACKGROUND Along with the unceasing progress of medicine,Crohn's disease(CD),especially complex CD,is no longer a taboo for minimally invasive surgery.However,considering its special disease characteristics,more clinical trials are needed to confirm the safety and feasibility of laparoscopic surgery for CD.AIM To investigate the safety and feasibility of laparoscopic enterectomy for CD,assess the advantages of laparoscopy over laparotomy in patients with CD,and discuss comprehensive minimally invasive surgical techniques in complex CD.METHODS This study prospectively collected clinical data from patients with CD who underwent enterectomy from January 2017 to January 2020.It was registered in the Chinese clinical trial database with the registration number ChiCTR-INR-16009321.Patients were divided into a laparoscopy group and a traditional laparotomy group according to the surgical method.The baseline characteristics,operation time,intraoperative blood loss,temporary stoma,levels of abdominal adhesion,pathological characteristics,days to flatus and soft diet,postoperative complications,hospitalization time,readmission rate within 30 d,and hospitalization cost were compared between the two groups.RESULTS A total of 120 eligible patients were enrolled into the pre-standardized groups,including 100 in the laparoscopy group and 20 in the laparotomy group.Compared with the laparotomy group,the postoperative hospitalization time in the laparoscopy group was shorter(9.1±3.9 d vs 11.0±1.6 d,P<0.05),the days to flatus were fewer(2.8±0.8 d vs 3.5±0.7 d,P<0.05),the days to soft diet were fewer(4.2±2.4 d vs 6.2±2.0 d,P<0.05)and the intraoperative blood loss was less(103.3±80.42 mL vs 169.5±100.42 mL,P<0.05).There were no statistically significant differences between the two groups in preoperative clinical data,operation time(149.0±43.8 min vs 159.2±40.0 min),stoma rate,levels of abdominal adhesion,total cost of hospitalization,incidence of postoperative complications[8.0%(8/100)vs 15.0%(3/20)],or readmission rate wi展开更多
BACKGROUND The purpose of enhanced recovery after surgery(ERAS)was to reduce surgical pressure and accelerate postoperative functional recovery.Although the application of biologics in treating inflammatory bowel dise...BACKGROUND The purpose of enhanced recovery after surgery(ERAS)was to reduce surgical pressure and accelerate postoperative functional recovery.Although the application of biologics in treating inflammatory bowel disease(IBD)has changed treatment strategies,most patients with IBD still require surgery.AIM To evaluate the advantage of ERAS in IBD surgery.METHODS The PubMed,EMBASE and Cochrane Library databases were searched from inception to March 21,2021 to find eligible studies.The primary outcome was postoperative complications,and the secondary outcomes included operation time,time to first flatus,time to bowel movement,postoperative hospital stay and readmission.The PROSPERO registration ID of this meta-analysis is CRD42021238052.RESULTS A total of eight studies involving 1939 patients were included in this metaanalysis.There were no differences in baseline information between the ERAS group and the non-ERAS group.After pooling up all of the data,no significant difference was found between the ERAS group and the non-ERAS group in terms of postoperative overall complications[odds ratio=0.82,95%confidence interval(CI)=0.66 to 1.02,P=0.08].The ERAS group had a lower prevalence of anastomotic fistula(odds ratio=0.36,95%CI=0.13 to 0.95,P=0.04),less time to first flatus[mean difference(MD)=-2.03,95%CI=-3.89 to-0.17,P=0.03],less time to bowel movement(MD=-1.08,95%CI=-1.60 to-0.57,P<0.01)and shorter postoperative hospital stays(MD=-1.99,95%CI=-3.27 to-0.71,P<0.01)than the non-ERAS group. CONCLUSIONERAS was effective for the quicker recovery in IBD surgery and did not lead to increased complications.展开更多
Purpose: Water consumption and vegetative growth in resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars infected with Verticillium dahliae were assessed. Methods: Plants were inoculated with the path...Purpose: Water consumption and vegetative growth in resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars infected with Verticillium dahliae were assessed. Methods: Plants were inoculated with the pathogen and disease evaluated in growth chamber. Mean daily water consumption and vegetative growth were recorded during 13 weeks. Results: Water consumption significantly decreased in inoculated plants compared to non-inoculated plants during the 3rd - 6th weeks after inoculation period. From the 9th week, a significantly lower consumption occurred in infected “Picual” plants compared with “Frantoio” plants. Vegetative growth was reduced in inoculated plants of both cultivars, although the reduction of the fresh weight was remarkably higher in “Picual” compared with controls due to defoliation and tissue desiccation, impeding the production of new shoots and leaves. Conversely, in “Frantoio”, defoliation was significantly reduced to low levels. Conclusions: Water consumption and vegetative growth in plants of resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars inoculated by Verticillium dahliae were consistently associated with the progress of the wilt symptoms and the level of resistance of the cultivar. Growth and water consumption reductions in “Picual” were irreversible in almost all plants, while “Frantoio” plants were able to maintain water translocation, overcome the infection and avoid vascular occlusion, and restart vegetative growth.展开更多
基金supported by grants from the National High-Tech Development Project of Ministry of Sciences and Technology of China(2012AA020703)the National Natural Science Foundation of China(31472056)+1 种基金Scientific Project of the Science and Technology Department of Guangxi Zhuang Autonomous Region,China(1598025-31)Scientific Project of the Science and Technology Bureau of Nanning Municipality,Guangxi Zhuang Autonomous Region,China(20145194,20155192)
文摘In this study, we developed a systemic PD model in middle-aged cynomolgus monkeys using individualized low-dose MPTP, to explore effective indicators for the early prediction of clinical outcomes. MPTP was not stopped until the animals showed typical PD motor symptoms on days 10 to 13 after MPTP administration when the Kurlan score reached 10; this abrogated the dif- ferences in individual susceptibility to MPTP. The clinical symptoms persisted, peaking on days 3 to 12 after MPTP withdrawal （rapid progress stage）, and then the Kurlan score plateaued. A Kurlan score at the end of the rapid progress stage 〉15 reflected stable or slowly-progressive PD, while a score 〈15 indicated spontaneous recovery. The entire clinical evolution and outcome of the systemic PD model was characterized in this study, thus providing options for therapeutic and translational research.
文摘BACKGROUND Along with the unceasing progress of medicine,Crohn's disease(CD),especially complex CD,is no longer a taboo for minimally invasive surgery.However,considering its special disease characteristics,more clinical trials are needed to confirm the safety and feasibility of laparoscopic surgery for CD.AIM To investigate the safety and feasibility of laparoscopic enterectomy for CD,assess the advantages of laparoscopy over laparotomy in patients with CD,and discuss comprehensive minimally invasive surgical techniques in complex CD.METHODS This study prospectively collected clinical data from patients with CD who underwent enterectomy from January 2017 to January 2020.It was registered in the Chinese clinical trial database with the registration number ChiCTR-INR-16009321.Patients were divided into a laparoscopy group and a traditional laparotomy group according to the surgical method.The baseline characteristics,operation time,intraoperative blood loss,temporary stoma,levels of abdominal adhesion,pathological characteristics,days to flatus and soft diet,postoperative complications,hospitalization time,readmission rate within 30 d,and hospitalization cost were compared between the two groups.RESULTS A total of 120 eligible patients were enrolled into the pre-standardized groups,including 100 in the laparoscopy group and 20 in the laparotomy group.Compared with the laparotomy group,the postoperative hospitalization time in the laparoscopy group was shorter(9.1±3.9 d vs 11.0±1.6 d,P<0.05),the days to flatus were fewer(2.8±0.8 d vs 3.5±0.7 d,P<0.05),the days to soft diet were fewer(4.2±2.4 d vs 6.2±2.0 d,P<0.05)and the intraoperative blood loss was less(103.3±80.42 mL vs 169.5±100.42 mL,P<0.05).There were no statistically significant differences between the two groups in preoperative clinical data,operation time(149.0±43.8 min vs 159.2±40.0 min),stoma rate,levels of abdominal adhesion,total cost of hospitalization,incidence of postoperative complications[8.0%(8/100)vs 15.0%(3/20)],or readmission rate wi
文摘BACKGROUND The purpose of enhanced recovery after surgery(ERAS)was to reduce surgical pressure and accelerate postoperative functional recovery.Although the application of biologics in treating inflammatory bowel disease(IBD)has changed treatment strategies,most patients with IBD still require surgery.AIM To evaluate the advantage of ERAS in IBD surgery.METHODS The PubMed,EMBASE and Cochrane Library databases were searched from inception to March 21,2021 to find eligible studies.The primary outcome was postoperative complications,and the secondary outcomes included operation time,time to first flatus,time to bowel movement,postoperative hospital stay and readmission.The PROSPERO registration ID of this meta-analysis is CRD42021238052.RESULTS A total of eight studies involving 1939 patients were included in this metaanalysis.There were no differences in baseline information between the ERAS group and the non-ERAS group.After pooling up all of the data,no significant difference was found between the ERAS group and the non-ERAS group in terms of postoperative overall complications[odds ratio=0.82,95%confidence interval(CI)=0.66 to 1.02,P=0.08].The ERAS group had a lower prevalence of anastomotic fistula(odds ratio=0.36,95%CI=0.13 to 0.95,P=0.04),less time to first flatus[mean difference(MD)=-2.03,95%CI=-3.89 to-0.17,P=0.03],less time to bowel movement(MD=-1.08,95%CI=-1.60 to-0.57,P<0.01)and shorter postoperative hospital stays(MD=-1.99,95%CI=-3.27 to-0.71,P<0.01)than the non-ERAS group. CONCLUSIONERAS was effective for the quicker recovery in IBD surgery and did not lead to increased complications.
文摘Purpose: Water consumption and vegetative growth in resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars infected with Verticillium dahliae were assessed. Methods: Plants were inoculated with the pathogen and disease evaluated in growth chamber. Mean daily water consumption and vegetative growth were recorded during 13 weeks. Results: Water consumption significantly decreased in inoculated plants compared to non-inoculated plants during the 3rd - 6th weeks after inoculation period. From the 9th week, a significantly lower consumption occurred in infected “Picual” plants compared with “Frantoio” plants. Vegetative growth was reduced in inoculated plants of both cultivars, although the reduction of the fresh weight was remarkably higher in “Picual” compared with controls due to defoliation and tissue desiccation, impeding the production of new shoots and leaves. Conversely, in “Frantoio”, defoliation was significantly reduced to low levels. Conclusions: Water consumption and vegetative growth in plants of resistant (“Frantoio”) and susceptible (“Picual”) olive cultivars inoculated by Verticillium dahliae were consistently associated with the progress of the wilt symptoms and the level of resistance of the cultivar. Growth and water consumption reductions in “Picual” were irreversible in almost all plants, while “Frantoio” plants were able to maintain water translocation, overcome the infection and avoid vascular occlusion, and restart vegetative growth.
