目的探究脑皮质内皮屏障抗原(EBA)及血管内皮生长因子(VEGF)在放射性血脑屏障损害条件下的动态变化规律,为临床提供参考。方法使用随机数字表法将48只清洁级雄性SD大鼠分为对照组和放射性脑损伤后7、14和28 d 4组,每组12只。采用X线电...目的探究脑皮质内皮屏障抗原(EBA)及血管内皮生长因子(VEGF)在放射性血脑屏障损害条件下的动态变化规律,为临床提供参考。方法使用随机数字表法将48只清洁级雄性SD大鼠分为对照组和放射性脑损伤后7、14和28 d 4组,每组12只。采用X线电子计算机断层扫描设备制备大鼠放射性脑损伤模型。大鼠按照3 m L/kg的剂量尾静脉注射3%伊文思蓝(EB),开颅并暴露脑皮质血管,放于微循环显微镜下观察EB渗出情况,并借助微循环显微成像系统评估血脑屏障通透性;使用免疫组化染色的方法来检测各组大鼠脑皮质EBA和VEGF的表达。结果与对照组相比,损伤组大鼠脑皮质微血管EB外渗量和VEGF的表达水平于伤后7、14、28 d均有不同程度升高(均P<0.05),从伤后7 d至28 d逐渐降低,损伤各亚组之间差异均有统计学意义(均P<0.05),二者呈正相关(r=0.898,P<0.001);EBA表达水平于伤后各个时间点均下降(均P<0.05),从伤后7 d至28 d逐渐升高,损伤各亚组之间差异均有统计学意义(均P<0.05),与EB外渗量呈负相关(r=-0.866,P<0.001)。结论大鼠放射性脑损伤后血脑屏障通透性增加与EBA表达减少、VEGF表达增加具有重要关联性。展开更多
Radiation therapy is considered the most effective non-surgical treatment for brain tumors.However,there are no available treatments for radiation-induced brain injury.Bisdemethoxycurcumin(BDMC)is a demethoxy derivati...Radiation therapy is considered the most effective non-surgical treatment for brain tumors.However,there are no available treatments for radiation-induced brain injury.Bisdemethoxycurcumin(BDMC)is a demethoxy derivative of curcumin that has anti-proliferative,anti-inflammatory,and anti-oxidant properties.To determine whether BDMC has the potential to treat radiation-induced brain injury,in this study,we established a rat model of radiation-induced brain injury by administe ring a single 30-Gy vertical dose of irradiation to the whole brain,followed by intraperitoneal injection of 500μL of a 100 mg/kg BDMC solution every day for 5 successive weeks.Our res ults showed that BDMC increased the body weight of rats with radiation-induced brain injury,improved lea rning and memory,attenuated brain edema,inhibited astrocyte activation,and reduced oxidative stress.These findings suggest that BDMC protects against radiationinduced brain injury.展开更多
文摘目的探究脑皮质内皮屏障抗原(EBA)及血管内皮生长因子(VEGF)在放射性血脑屏障损害条件下的动态变化规律,为临床提供参考。方法使用随机数字表法将48只清洁级雄性SD大鼠分为对照组和放射性脑损伤后7、14和28 d 4组,每组12只。采用X线电子计算机断层扫描设备制备大鼠放射性脑损伤模型。大鼠按照3 m L/kg的剂量尾静脉注射3%伊文思蓝(EB),开颅并暴露脑皮质血管,放于微循环显微镜下观察EB渗出情况,并借助微循环显微成像系统评估血脑屏障通透性;使用免疫组化染色的方法来检测各组大鼠脑皮质EBA和VEGF的表达。结果与对照组相比,损伤组大鼠脑皮质微血管EB外渗量和VEGF的表达水平于伤后7、14、28 d均有不同程度升高(均P<0.05),从伤后7 d至28 d逐渐降低,损伤各亚组之间差异均有统计学意义(均P<0.05),二者呈正相关(r=0.898,P<0.001);EBA表达水平于伤后各个时间点均下降(均P<0.05),从伤后7 d至28 d逐渐升高,损伤各亚组之间差异均有统计学意义(均P<0.05),与EB外渗量呈负相关(r=-0.866,P<0.001)。结论大鼠放射性脑损伤后血脑屏障通透性增加与EBA表达减少、VEGF表达增加具有重要关联性。
基金supported by the National Natural Science Foundation of China,No.82002400(to GJZ)Scientific Research Project of Hu nan Health Committee,No.20201911and No.20200469(both to ZJX)+2 种基金Scientific Research Project of Hunan Health Committee,No.20211411761(to HMW)the Natural Science Foundation of Hunan Province,No.2020JJ5512(to GJZ)a grant from Clinical Medical Technology Innovation Guidance Project in Hunan Province,No.2020SK51822(to ZJX)。
文摘Radiation therapy is considered the most effective non-surgical treatment for brain tumors.However,there are no available treatments for radiation-induced brain injury.Bisdemethoxycurcumin(BDMC)is a demethoxy derivative of curcumin that has anti-proliferative,anti-inflammatory,and anti-oxidant properties.To determine whether BDMC has the potential to treat radiation-induced brain injury,in this study,we established a rat model of radiation-induced brain injury by administe ring a single 30-Gy vertical dose of irradiation to the whole brain,followed by intraperitoneal injection of 500μL of a 100 mg/kg BDMC solution every day for 5 successive weeks.Our res ults showed that BDMC increased the body weight of rats with radiation-induced brain injury,improved lea rning and memory,attenuated brain edema,inhibited astrocyte activation,and reduced oxidative stress.These findings suggest that BDMC protects against radiationinduced brain injury.
