DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(T...DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(TRSV) and Cucumber mosaic virus(CMV) were designed and applied to the detection of the main potato viruses with multiplex reverse transcription polymerase chain reaction(m-RT-PCR),including two quarantine viruses in China(PVV and TRSV).The results showed that DPO-m-RT-PCR exhi-bited high PCR specificity and sensitivity even under less than optimal PCR condition compared with conventional m-RT-PCR.DPO-m-RT-PCR could be applied in the healthy evaluation of seed potato and its exit-entry quarantine.展开更多
A novel RT-PCR method integrated with Magnetic Nano Particles(MNP),MNP-RT-PCR,was set up for detection of Cucumber green mottle mosaic virus(CGMMV).After the virus particles in crude sap were concentrated by MNP,viral...A novel RT-PCR method integrated with Magnetic Nano Particles(MNP),MNP-RT-PCR,was set up for detection of Cucumber green mottle mosaic virus(CGMMV).After the virus particles in crude sap were concentrated by MNP,viral RNAs were released and were detected by RT-PCR.CGMMV could be detected in as less as 10 ng watermelon leaf materials.Compared with normal RT-PCR,the method decreased the inhibitors of plant material and steps for extracting RNA,and also increased the sensitivity of RT-PCR detection in less time.The method is simple and suitable for quick detection of plant virus in a large number of samples.展开更多
In this paper, a three-dimensional quantitative structure-activity relationships(3 D-QSAR) study for 20 HIV-1 reverse transcriptase(RT) inhibitors was established using Topomer Co MFA. The models were built based on d...In this paper, a three-dimensional quantitative structure-activity relationships(3 D-QSAR) study for 20 HIV-1 reverse transcriptase(RT) inhibitors was established using Topomer Co MFA. The models were built based on different fragment cutting models, with the most effective model of the multiple correlation coefficients of fitting(r^2) to be 0.920, cross-validation(q^2) of 0.575, and external validation(Q_(ext)~2) being 0.701. The results indicated that the model obtained has both favorable estimation stability and good prediction capability. Topomer Search was used to search R-group from ZINC database. As a result, a series of R-groups with relatively high activity contribution was obtained. By No. 6 molecule filtering, 3 R_1 and 15 R_2 groups were selected, and employed to alternately substitute for the R_1 and R_2 of sample 6. Finally, 45 new compounds were designed, and the Topomer CoMFA model was used to predicate the biological activity, so the Topomer Search is effective in screening and can guide the design of new HIV/AIDS drugs. The molecular docking method was also used to study the interactions of these drugs by docking the ligands into HIV-1 RT active site, which revealed the likely bioactive conformations. This study showed that there are extensive interactions between the 1,3,4-thiazolidinone revertase inhibitors and His84, Asp145, Lys33 and Leu83 residues in the active site of HIV-1 RT. These results provide useful insights for the design of potent new inhibitors of RT.展开更多
To investigate the possible role of interleukin (IL) 10 and IL 12 in the pathogenesis of psoriatic lesions and to supply theoretical basis for the gene therapy for psoriasis, the expression of IL 10 and IL 12 P35,...To investigate the possible role of interleukin (IL) 10 and IL 12 in the pathogenesis of psoriatic lesions and to supply theoretical basis for the gene therapy for psoriasis, the expression of IL 10 and IL 12 P35, P40 mRNA in 12 cases of psoriatic lesions and 6 normal skin tissues was detected by using RT PCR technique. The results showed that the expression of IL 10 mRNA in the psoriatic lesions was significantly lower than that in the normal skin tissues ( P <0.001). The expression of IL 12 P35 was positive both in the psoriatic lesions and in the normal skin tissues. IL 12 P40 mRNA was expressed positively only in the psoriatic lesions but negatively in the normal skin tissues. It was suggested that IL 12 might take an important role in the occurrence and progression of psoriasis, but IL 10 might have certain role in the regression of psoriasis.展开更多
A rectangular m × n resistor network with an arbitrary boundary is investigated, and a general resistance formula between two nodes on an arbitrary axis is derived by the Recursion-Transform（RT） method, a probl...A rectangular m × n resistor network with an arbitrary boundary is investigated, and a general resistance formula between two nodes on an arbitrary axis is derived by the Recursion-Transform（RT） method, a problem that has never been resolved before, for the Green＇s function technique and the Laplacian matrix approach are inapplicable to it. To have the exact solution of resistance is important but it is difficult to obtain under the condition of arbitrary boundary. Our result is directly expressed in a single summation and mainly composed of characteristic roots, which contain both finite and infinite cases. Further, the current distribution is given explicitly as a byproduct of the method. Our framework can be effectively applied to RLC networks. As an application to the LC network, we find that our formulation leads to the occurrence of resonances at h_1= 1-cosφ_i-sinφ_icotnφ_i. This somewhat curious result suggests the possibility of practical applications of our formulae to resonant circuits.展开更多
文摘DPO is one of the new specific primers for virus detection.In this study,five sets of dual priming oligonucleotide(DPO) primers for Potato virus X(PVX),Potato virus Y(PVY),Potato virus V(PVV),Tobacco ring spot virus(TRSV) and Cucumber mosaic virus(CMV) were designed and applied to the detection of the main potato viruses with multiplex reverse transcription polymerase chain reaction(m-RT-PCR),including two quarantine viruses in China(PVV and TRSV).The results showed that DPO-m-RT-PCR exhi-bited high PCR specificity and sensitivity even under less than optimal PCR condition compared with conventional m-RT-PCR.DPO-m-RT-PCR could be applied in the healthy evaluation of seed potato and its exit-entry quarantine.
文摘A novel RT-PCR method integrated with Magnetic Nano Particles(MNP),MNP-RT-PCR,was set up for detection of Cucumber green mottle mosaic virus(CGMMV).After the virus particles in crude sap were concentrated by MNP,viral RNAs were released and were detected by RT-PCR.CGMMV could be detected in as less as 10 ng watermelon leaf materials.Compared with normal RT-PCR,the method decreased the inhibitors of plant material and steps for extracting RNA,and also increased the sensitivity of RT-PCR detection in less time.The method is simple and suitable for quick detection of plant virus in a large number of samples.
基金supported by the National Natural Science Foundation of China(21475081,21275094)the Graduate Innovation Fund of Shaanxi University of Science and Technology
文摘In this paper, a three-dimensional quantitative structure-activity relationships(3 D-QSAR) study for 20 HIV-1 reverse transcriptase(RT) inhibitors was established using Topomer Co MFA. The models were built based on different fragment cutting models, with the most effective model of the multiple correlation coefficients of fitting(r^2) to be 0.920, cross-validation(q^2) of 0.575, and external validation(Q_(ext)~2) being 0.701. The results indicated that the model obtained has both favorable estimation stability and good prediction capability. Topomer Search was used to search R-group from ZINC database. As a result, a series of R-groups with relatively high activity contribution was obtained. By No. 6 molecule filtering, 3 R_1 and 15 R_2 groups were selected, and employed to alternately substitute for the R_1 and R_2 of sample 6. Finally, 45 new compounds were designed, and the Topomer CoMFA model was used to predicate the biological activity, so the Topomer Search is effective in screening and can guide the design of new HIV/AIDS drugs. The molecular docking method was also used to study the interactions of these drugs by docking the ligands into HIV-1 RT active site, which revealed the likely bioactive conformations. This study showed that there are extensive interactions between the 1,3,4-thiazolidinone revertase inhibitors and His84, Asp145, Lys33 and Leu83 residues in the active site of HIV-1 RT. These results provide useful insights for the design of potent new inhibitors of RT.
文摘To investigate the possible role of interleukin (IL) 10 and IL 12 in the pathogenesis of psoriatic lesions and to supply theoretical basis for the gene therapy for psoriasis, the expression of IL 10 and IL 12 P35, P40 mRNA in 12 cases of psoriatic lesions and 6 normal skin tissues was detected by using RT PCR technique. The results showed that the expression of IL 10 mRNA in the psoriatic lesions was significantly lower than that in the normal skin tissues ( P <0.001). The expression of IL 12 P35 was positive both in the psoriatic lesions and in the normal skin tissues. IL 12 P40 mRNA was expressed positively only in the psoriatic lesions but negatively in the normal skin tissues. It was suggested that IL 12 might take an important role in the occurrence and progression of psoriasis, but IL 10 might have certain role in the regression of psoriasis.
基金Project supported by the Prophase Preparatory Project of Natural Science Foundation of Nantong University,China(Grant No.15ZY16)
文摘A rectangular m × n resistor network with an arbitrary boundary is investigated, and a general resistance formula between two nodes on an arbitrary axis is derived by the Recursion-Transform（RT） method, a problem that has never been resolved before, for the Green＇s function technique and the Laplacian matrix approach are inapplicable to it. To have the exact solution of resistance is important but it is difficult to obtain under the condition of arbitrary boundary. Our result is directly expressed in a single summation and mainly composed of characteristic roots, which contain both finite and infinite cases. Further, the current distribution is given explicitly as a byproduct of the method. Our framework can be effectively applied to RLC networks. As an application to the LC network, we find that our formulation leads to the occurrence of resonances at h_1= 1-cosφ_i-sinφ_icotnφ_i. This somewhat curious result suggests the possibility of practical applications of our formulae to resonant circuits.
