2006年5月以来,我国部分猪场暴发了一种以高热、高发病率和高死亡率为特征的传染性疾病,经病原分离及分子流行病学分析证明是由一种带有nsp2部分缺失的、对猪呈高致病性的猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory ...2006年5月以来,我国部分猪场暴发了一种以高热、高发病率和高死亡率为特征的传染性疾病,经病原分离及分子流行病学分析证明是由一种带有nsp2部分缺失的、对猪呈高致病性的猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)引起的。本实验参考GenBank发表的以及本实验室分离鉴定的PRRSV的nsp2基因序列,在nsp2缺失区的两端的保守区设计并合成了一对引物,建立了一种PRRSV的RT-PCR检测方法。该方法扩增高致病性PRRSV基因组时可获得230bp的片段,扩增经典型PRRSV时则获得320bp的片段,根据RT-PCR产物大小可将二者区分开来。通过大量临床病料的检测,并配合PCR产物测序验证,结果表明该方法简便、快速、特异,可以鉴别高致病性PRRSV,为进一步的PRRS流行病学研究提供了重要的技术手段。展开更多
According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of P...According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of PCV2 and PRRSV, respectively. With the established methods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2, 27 samples were positive for PRRSV and among the above positive samples, 18 samples were positive for both viruses. The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratory diseases.展开更多
文摘According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of PCV2 and PRRSV, respectively. With the established methods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2, 27 samples were positive for PRRSV and among the above positive samples, 18 samples were positive for both viruses. The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratory diseases.
