The aim of this study is to explore whether RT-5, one novel active ginsenosides from Ginseng, has protective effects against cisplatin（CDDP）-induced nephrotoxicity in vivo. One model of acute renal failure induced b...The aim of this study is to explore whether RT-5, one novel active ginsenosides from Ginseng, has protective effects against cisplatin（CDDP）-induced nephrotoxicity in vivo. One model of acute renal failure induced by CDDP in rats and one model of xenograft tumors of human cervical cancer in nude mice are established. Four groups are assigned： control, CDDP, RT-5, CDDP plus RT-5, in which the RT-5 is administered via oral gavage 24 h before CDDP intraperitoneal injection. The significant increase in blood urea nitrogen and creatinine are induced by CDDP treatment at 6 mg/kg, which are attenuated by pre-treated with RT-5 at 10 mg/kg. RT-5 could ameliorate CDDP-induced morphological damages in kidney by PAS staining, and reduce tubular apoptosis evaluated by TUNEL staining. Pretreatment with RT-5 notably inhibits CDDP-induced oxidative stress in kidney tissues. Interestingly, RT-5 does not interfere with the in vivo anti-cancer effects of CDDP against the growth of xenograft tumors in nude mice. These data suggest that co-treatment RT-5 with CDDP might attenuate the following nephrotoxicity without inhibiting its anti-tumor efficiency, which could provide one novel strategy for cancer treatment in clinics.展开更多
Objective To study the chemical constituents in the dried roots of Panax notoginseng.Methods The constituents were isolated and purified with chromatographic methods.Their structures were elucidated by spectroscopic m...Objective To study the chemical constituents in the dried roots of Panax notoginseng.Methods The constituents were isolated and purified with chromatographic methods.Their structures were elucidated by spectroscopic methods（1D,2D NMR,UV,IR,[α]D,and HRESI-TOF-MS）and chemical analyses.Results Twenty saponins including 20（S）-ginsenoside Rh1（1）,6-O-β-D-（6′-acetyl）-glucopyranosyl-24-ene-dammar-3β,6α,12β,20S-tetraol（2）,ginseno-side Rf（3）,notoginsenoside R2（4）,ginsenoside Rg2（5）,ginsenoside Rg1（6）,notoginsenoside Rt（7）,koryoginsenoside R1（8）,6-O-（β-D-glucopyranosyl）-20-O-（β-D-xylopyranosyl）-3β,6α,12β,20（S）-tetrahydroxy-dammar-24-ene（9）,pseudoginsenoside Rt3（10）,notoginsenoside R1（11）,ginsenoside Re（12）,notoginsenoside N（13）,ginsenoside F1（14）,ginsenoside U（15）,ginsenoside Rk3（16）,3β,12β-dihydroxydammar-（E）-20（22）,24-diene-6-O-β-D-xylopyranosyl-（1→2）-β-D-glucopyranoside（17）,ginsenoside Rh4（18）,pseudoginsenoside Rt5（19）,and vinaginsenoside R22（20）were obtained.Conclusion Compounds 2,19,and 20 are isolated from this species for the first time.The 1H-NMR data of compound 19 and1H-NMR and 13C-NMR data of compound 20 are first reported.Meanwhile,the NMR data ofβ-D-xylopyranosyl group in compound 9 is corrected.展开更多
An ocotillone type ginsenoside, together with 2 known ginsenosides was isolated from leaves of Panax ginseng and identified as pseudoginsenoside RT 5 on the basis of chemical and physicochemical evidences. It h...An ocotillone type ginsenoside, together with 2 known ginsenosides was isolated from leaves of Panax ginseng and identified as pseudoginsenoside RT 5 on the basis of chemical and physicochemical evidences. It has been so far the first example of ocotillone type ginsenoside discovered in Panax ginseng and its plausible biotransformation pathway also discussed.展开更多
基金Supported by the National Natural Science Foundation of China(81202038,81441130)the Taishan Scholar Project,Shandong Province Higher Educational Science and Technology Program(J12LM53)
文摘The aim of this study is to explore whether RT-5, one novel active ginsenosides from Ginseng, has protective effects against cisplatin（CDDP）-induced nephrotoxicity in vivo. One model of acute renal failure induced by CDDP in rats and one model of xenograft tumors of human cervical cancer in nude mice are established. Four groups are assigned： control, CDDP, RT-5, CDDP plus RT-5, in which the RT-5 is administered via oral gavage 24 h before CDDP intraperitoneal injection. The significant increase in blood urea nitrogen and creatinine are induced by CDDP treatment at 6 mg/kg, which are attenuated by pre-treated with RT-5 at 10 mg/kg. RT-5 could ameliorate CDDP-induced morphological damages in kidney by PAS staining, and reduce tubular apoptosis evaluated by TUNEL staining. Pretreatment with RT-5 notably inhibits CDDP-induced oxidative stress in kidney tissues. Interestingly, RT-5 does not interfere with the in vivo anti-cancer effects of CDDP against the growth of xenograft tumors in nude mice. These data suggest that co-treatment RT-5 with CDDP might attenuate the following nephrotoxicity without inhibiting its anti-tumor efficiency, which could provide one novel strategy for cancer treatment in clinics.
基金Fund: Important Drug Development Fund, Ministry of Science and Technology of China (2012ZX09101201-002)Research Fund for the Doctoral Program of Higher Education of China (20121210110007)
文摘Objective To study the chemical constituents in the dried roots of Panax notoginseng.Methods The constituents were isolated and purified with chromatographic methods.Their structures were elucidated by spectroscopic methods（1D,2D NMR,UV,IR,[α]D,and HRESI-TOF-MS）and chemical analyses.Results Twenty saponins including 20（S）-ginsenoside Rh1（1）,6-O-β-D-（6′-acetyl）-glucopyranosyl-24-ene-dammar-3β,6α,12β,20S-tetraol（2）,ginseno-side Rf（3）,notoginsenoside R2（4）,ginsenoside Rg2（5）,ginsenoside Rg1（6）,notoginsenoside Rt（7）,koryoginsenoside R1（8）,6-O-（β-D-glucopyranosyl）-20-O-（β-D-xylopyranosyl）-3β,6α,12β,20（S）-tetrahydroxy-dammar-24-ene（9）,pseudoginsenoside Rt3（10）,notoginsenoside R1（11）,ginsenoside Re（12）,notoginsenoside N（13）,ginsenoside F1（14）,ginsenoside U（15）,ginsenoside Rk3（16）,3β,12β-dihydroxydammar-（E）-20（22）,24-diene-6-O-β-D-xylopyranosyl-（1→2）-β-D-glucopyranoside（17）,ginsenoside Rh4（18）,pseudoginsenoside Rt5（19）,and vinaginsenoside R22（20）were obtained.Conclusion Compounds 2,19,and 20 are isolated from this species for the first time.The 1H-NMR data of compound 19 and1H-NMR and 13C-NMR data of compound 20 are first reported.Meanwhile,the NMR data ofβ-D-xylopyranosyl group in compound 9 is corrected.
文摘An ocotillone type ginsenoside, together with 2 known ginsenosides was isolated from leaves of Panax ginseng and identified as pseudoginsenoside RT 5 on the basis of chemical and physicochemical evidences. It has been so far the first example of ocotillone type ginsenoside discovered in Panax ginseng and its plausible biotransformation pathway also discussed.
