Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8%...Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8% were used during an 8-week feeding trial to evaluate its effects on RNA/DNA ratio and lipoprotein lipase(LPL) and Myo D m RNA expressions in muscle of Oreochromis niloticus.The results showed that RNA,DNA content as well as ratio of RNA to DNA were significantly affected(P < 0.05),in each case the highest was recorded in fish group subjected to 6% palm oil level.There was a strong positive correlation between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and specific growth rate(SGR),protein efficiency ratio(PER),while a negative correlation existed between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and feed conversion ratio(FCR).The m RNA expressions of LPL and Myo D in muscle were not significantly affected by the different palm oil levels,although the highest expression was observed in fish fed with 6% palm oil level.There also existed a strong positive correlation between the m RNA expression of LPL,Myo D and SGR,PER,while their correlation with FCR was negative.In conclusion,elevated palm oil affected the RNA,DNA concentration as well as RNA/DNA ratio significantly,although the m RNA expression of LPL and Myo D were not affected significantly by elevated palm oil levels.展开更多
为优化碱解法测定中华鳖肝脏组织RNA-DNA含量比值的操作流程,选用中华鳖稚鳖的肝脏组织,在Buckley(1979)和Robbins(1990)方法的基础上,分别针对以往操作流程中匀浆介质、离心速度、碱解水浴时间和碱解量的差别进行实验.结果发现,使用由0...为优化碱解法测定中华鳖肝脏组织RNA-DNA含量比值的操作流程,选用中华鳖稚鳖的肝脏组织,在Buckley(1979)和Robbins(1990)方法的基础上,分别针对以往操作流程中匀浆介质、离心速度、碱解水浴时间和碱解量的差别进行实验.结果发现,使用由0.05 mol/L Tris-HCl+0.005 mol/L EDTA(p H7.4)组成的匀浆介质,12 000×g的离心速率,37℃碱解水浴2 h以及0.56 m L 0.3 mol/L KOH+0.25 m L 1.5 mol/L PCA的碱解酸化组合能有效提高碱解法测定中华鳖肝脏组织RNA-DNA含量比值的稳定性.展开更多
基金supported by China Agriculture Research System (CARS-49)Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206)
文摘Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8% were used during an 8-week feeding trial to evaluate its effects on RNA/DNA ratio and lipoprotein lipase(LPL) and Myo D m RNA expressions in muscle of Oreochromis niloticus.The results showed that RNA,DNA content as well as ratio of RNA to DNA were significantly affected(P < 0.05),in each case the highest was recorded in fish group subjected to 6% palm oil level.There was a strong positive correlation between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and specific growth rate(SGR),protein efficiency ratio(PER),while a negative correlation existed between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and feed conversion ratio(FCR).The m RNA expressions of LPL and Myo D in muscle were not significantly affected by the different palm oil levels,although the highest expression was observed in fish fed with 6% palm oil level.There also existed a strong positive correlation between the m RNA expression of LPL,Myo D and SGR,PER,while their correlation with FCR was negative.In conclusion,elevated palm oil affected the RNA,DNA concentration as well as RNA/DNA ratio significantly,although the m RNA expression of LPL and Myo D were not affected significantly by elevated palm oil levels.
文摘为优化碱解法测定中华鳖肝脏组织RNA-DNA含量比值的操作流程,选用中华鳖稚鳖的肝脏组织,在Buckley(1979)和Robbins(1990)方法的基础上,分别针对以往操作流程中匀浆介质、离心速度、碱解水浴时间和碱解量的差别进行实验.结果发现,使用由0.05 mol/L Tris-HCl+0.005 mol/L EDTA(p H7.4)组成的匀浆介质,12 000×g的离心速率,37℃碱解水浴2 h以及0.56 m L 0.3 mol/L KOH+0.25 m L 1.5 mol/L PCA的碱解酸化组合能有效提高碱解法测定中华鳖肝脏组织RNA-DNA含量比值的稳定性.
