目的探讨贝那普利治疗系膜增生性肾小球肾炎(Ms PGN)的机制。方法选用2月龄SD雄性大鼠49只,适应性喂养1周后,按单纯随机抽样方法,抽取10只大鼠作为空白对照组,剩余39只大鼠建立Ms PGN模型后随机分为模型组、对照组(缬沙坦组)、实验组(...目的探讨贝那普利治疗系膜增生性肾小球肾炎(Ms PGN)的机制。方法选用2月龄SD雄性大鼠49只,适应性喂养1周后,按单纯随机抽样方法,抽取10只大鼠作为空白对照组,剩余39只大鼠建立Ms PGN模型后随机分为模型组、对照组(缬沙坦组)、实验组(贝那普利组)。对照组每日给予缬沙坦水溶液8.4 mg/kg灌胃,实验组每日给予贝那普利水溶液1.5 mg/kg灌胃,空白对照组和模型组每日给予等量生理盐水灌胃,连续灌胃12周。给药后0周、6周、12周后对24 h尿液进行收集,记录24 h尿蛋白定量(24 h UP)。采用酶联免疫吸附法测定血清基质金属蛋白酶-9(MMP-9)和金属蛋白酶组织抑制剂-1(TIMP-1)水平,采用免疫组织化学法检测大鼠肾组织MMP-9和TIMP-1蛋白表达水平。结果模型组大鼠给药0、6、12周后24 h UP均显著高于空白对照组(P<0.05)。随着治疗时间延长,对照组和实验组大鼠24 h UP均逐步下降,其中给药12周后实验组大鼠24 h UP显著低于对照组(P<0.05)。模型组大鼠血清和肾组织MMP-9、TIMP-1水平显著高于空白对照组(P<0.05),MMP-9/TIMP-1值显著低于空白对照组(P<0.05)。实验组大鼠血清和肾组织MMP-9表达水平和MMP-9/TIMP-1值显著高于模型组(P<0.05),TIMP-1表达水平显著低于模型组(P<0.05);实验组大鼠血清和肾组织MMP-9/TIMP-1值显著高于对照组(P<0.05)。结论贝那普利可通过上调MMP-9水平,抑制TIMP-1水平,发挥其对Ms PGN的治疗作用。展开更多
Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigati...Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigating these diseases is increasing and becoming expensively high. There are many ways to explore allergenic antibodies to assess the presence the amount of specific IgE as: Skin test (Prick), Specific IgE (ELISA), RAST Sp.IgE and Elimination Challenge method. Skin test produces pain, local and anaphylactic reaction and patient discomfort, other procedure with highly cost to patient. So, we have built up an evidence to use it in our allergy and immunology clinic has suitable cost for all patients based about for the other tests but it was very simple, accurate, cheap and does not produce any problems to patients. Our test depends on ELISA technique, it measures the quantitative amount of the following different allergens: As regard food allergens: Milk, Eggs, Banana, Maize, Fish, Chocolate, Wheat, Nuts, Strawberry, Shrimps, Spices, with drug as Aspirin. As regard inhalants as: House dust, Mite, Mixed Pollens, Mixed Moulds, Hay dust, Wool, Latex and Cat Hair. 150 allergic patient results of our test compared with specific IgE national kits (ELISA), Sp.IgE (RAST), Skin test and elimination challenge test. The statistical evaluation results as regard senistivity respectively: 88.9%, 89.6%, 91.2%, 71.4%, 93.1%. As regard specificity the results: 93.1%, 94.7%, 95.3%, 65.5%, 91.6%, the latter results showed that our test was in line with all tests. Our test was probably in food, drug and inhalant allergens, it can be also noted that the cheapest and most commercial technique was used, so as to be available immediately in standard laboratory and its reagents, plate and other requirements were prepared locally in our laboratory. Now it was applying successfully in allergy and immunology unit Ain Shams university hospitals in Egypt.展开更多
Six grades of biopolymers formulated to have oil content of 40% (M1), 50% (M2), and 60% (M3) melon seed oil (MESO) and 40% (C1), 50% (C2), and 60% (C3) cottonseed oil (COSO) respectively, were prepared with phthalic a...Six grades of biopolymers formulated to have oil content of 40% (M1), 50% (M2), and 60% (M3) melon seed oil (MESO) and 40% (C1), 50% (C2), and 60% (C3) cottonseed oil (COSO) respectively, were prepared with phthalic anhydride, and glycerol using alcoholysis-polycondensation process. The extend of polycondensation was monitored by determining the acid value of aliquots of the reaction mixture at various intervals of time. Molecular weight averages and polydispersity index (PDI) of the finished alkyds were determined by Rast method and end-group analysis. Molecular weight averages and PDI vary with differences in oil length of the alkyds, with samples M2 and C2 respectively exhibiting the highest PDI. Molecular weight average obtained from end-group analysis and those determined by Rast method in brackets are 1338.92 (597.00), 982.33 (696.25), 1316.09 (754.03), and 1160.57 (448.13), 765.96 (583.57), 1049.92 (696.25) for samples M1, M2, M3 and C1, C2, C3 respectively. Number molecular weight averages calculated from end-group analysis are larger than those obtained by Rast method for both MESO and COSO alkyds and seem to grossly overestimate their molecular weights. The mode of variation of these properties indicates that the synthesis of MESO and COSO alkyds are complex. Correlation of PDI with the quality of the finished alkyds shows that the higher the PDI value the better the quality of the alkyd. Performance properties such as rate of drying, film hardness and resistance to chemicals were optimum at 50% oil length for both triglyceride oil alkyds.展开更多
In the present study,Paenibacillus lautus strain BHU3 isolated from landfill soil was evaluated for the presence of potential endoglucanases which are the first candidate of cellulase enzyme system to act on cellulose...In the present study,Paenibacillus lautus strain BHU3 isolated from landfill soil was evaluated for the presence of potential endoglucanases which are the first candidate of cellulase enzyme system to act on cellulose.In-silico analysis revealed high potential thermostable endoglucanases which can efficiently interact with cellulose.The most potent and thermostable endoglucanase(locus tag id.CPZ30_18280)belonged to glycosyl family-5 and had interaction energy of−12.981 kcal/mol for the best docked cluster containing three out of ten docking conformations,and Tm value of 73.3℃.MD simulation of 100 ns proved highly stable binding interactions of CPZ30_18280 endoglucanase with cellulose with root mean square deviation(RMSD)values ranging from 0.15 to 0.30 nm.Consistent interactions with characteristic active site residues(tyrosine,tryptophan and aspartate)of glycosyl family-5 endoglucanases were found.Further,to enhance the production of endoglucanases,the fermentation conditions were optimized employing approaches like one factor at a time(OFAT)and response surface methodology(RSM).Maximum activity of endoglucanase was determined at 60℃.The optimized condi-tions for enhanced production of endoglucanase(10.15 U/mL)were pH 6.63,yeast extract conc.3.44 g/L,wheat bran 3.59%,and inoculum size 2.65%.Hence,P.lautus strain BHU3 has enormous potential to synthesize highly efficient thermostable endoglucanases under optimized regime using agro-wastes.Thus,it could find immense industrial applications including large scale cellulose conversion to bioethanol.展开更多
作者在原制备Der f Ⅰ变应原基础上进行其免疫化学特征鉴定。Der fⅠ的交叉免疫电泳(CIE)结果证实提纯的Der f Ⅰ为均质性。Der f Ⅰ氨基酸组成与Dandeu(1982)、Heymann(1986)报道的Der f Ⅰ相应结果相关性比较表明,三者在氨基酸组成上...作者在原制备Der f Ⅰ变应原基础上进行其免疫化学特征鉴定。Der fⅠ的交叉免疫电泳(CIE)结果证实提纯的Der f Ⅰ为均质性。Der f Ⅰ氨基酸组成与Dandeu(1982)、Heymann(1986)报道的Der f Ⅰ相应结果相关性比较表明,三者在氨基酸组成上有相似性。采用放射性变应原吸附试验(RAST)检测55份螨过敏性患者血清抗Der f ⅠIgE抗体水平,其中49.09％病人RAST阳性,平均阳性特异性IgE水平为6.58±3.90百分结合率。结果表明Der f Ⅰ是粉尘螨主要的变应原。展开更多
The heat transfer and flow characteristics of air jet impingement on a curved surface are investigated with computational fluid dynamics(CFD)approach.The first applied model is a one-equation SGS model for large eddy ...The heat transfer and flow characteristics of air jet impingement on a curved surface are investigated with computational fluid dynamics(CFD)approach.The first applied model is a one-equation SGS model for large eddy simulation(LES)and the second one is the SST-SAS hybrid RANS-LES.These models are utilized to study the flow physics in impinging process on a curved surface for different jet-to-surface(h/B)distances at two Reynolds numbers namely,2960 and 4740 based on the jet exit velocity(U_e)and the hydraulic diameter(2B).The predictions are compared with the experimental data in the literature and also the results from RANS k-εmodel.Comparisons show that both models can produce relatively good results.However,one-equation model(OEM)produced more accurate results especially at impingement region at lower jet-to-surface distances.In terms of heat transfer,the OEM also predicted better at different jet-to-surface spacings.It is also observed that both models show similar performance at higher h/B ratios.展开更多
文摘目的探讨贝那普利治疗系膜增生性肾小球肾炎(Ms PGN)的机制。方法选用2月龄SD雄性大鼠49只,适应性喂养1周后,按单纯随机抽样方法,抽取10只大鼠作为空白对照组,剩余39只大鼠建立Ms PGN模型后随机分为模型组、对照组(缬沙坦组)、实验组(贝那普利组)。对照组每日给予缬沙坦水溶液8.4 mg/kg灌胃,实验组每日给予贝那普利水溶液1.5 mg/kg灌胃,空白对照组和模型组每日给予等量生理盐水灌胃,连续灌胃12周。给药后0周、6周、12周后对24 h尿液进行收集,记录24 h尿蛋白定量(24 h UP)。采用酶联免疫吸附法测定血清基质金属蛋白酶-9(MMP-9)和金属蛋白酶组织抑制剂-1(TIMP-1)水平,采用免疫组织化学法检测大鼠肾组织MMP-9和TIMP-1蛋白表达水平。结果模型组大鼠给药0、6、12周后24 h UP均显著高于空白对照组(P<0.05)。随着治疗时间延长,对照组和实验组大鼠24 h UP均逐步下降,其中给药12周后实验组大鼠24 h UP显著低于对照组(P<0.05)。模型组大鼠血清和肾组织MMP-9、TIMP-1水平显著高于空白对照组(P<0.05),MMP-9/TIMP-1值显著低于空白对照组(P<0.05)。实验组大鼠血清和肾组织MMP-9表达水平和MMP-9/TIMP-1值显著高于模型组(P<0.05),TIMP-1表达水平显著低于模型组(P<0.05);实验组大鼠血清和肾组织MMP-9/TIMP-1值显著高于对照组(P<0.05)。结论贝那普利可通过上调MMP-9水平,抑制TIMP-1水平,发挥其对Ms PGN的治疗作用。
文摘Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigating these diseases is increasing and becoming expensively high. There are many ways to explore allergenic antibodies to assess the presence the amount of specific IgE as: Skin test (Prick), Specific IgE (ELISA), RAST Sp.IgE and Elimination Challenge method. Skin test produces pain, local and anaphylactic reaction and patient discomfort, other procedure with highly cost to patient. So, we have built up an evidence to use it in our allergy and immunology clinic has suitable cost for all patients based about for the other tests but it was very simple, accurate, cheap and does not produce any problems to patients. Our test depends on ELISA technique, it measures the quantitative amount of the following different allergens: As regard food allergens: Milk, Eggs, Banana, Maize, Fish, Chocolate, Wheat, Nuts, Strawberry, Shrimps, Spices, with drug as Aspirin. As regard inhalants as: House dust, Mite, Mixed Pollens, Mixed Moulds, Hay dust, Wool, Latex and Cat Hair. 150 allergic patient results of our test compared with specific IgE national kits (ELISA), Sp.IgE (RAST), Skin test and elimination challenge test. The statistical evaluation results as regard senistivity respectively: 88.9%, 89.6%, 91.2%, 71.4%, 93.1%. As regard specificity the results: 93.1%, 94.7%, 95.3%, 65.5%, 91.6%, the latter results showed that our test was in line with all tests. Our test was probably in food, drug and inhalant allergens, it can be also noted that the cheapest and most commercial technique was used, so as to be available immediately in standard laboratory and its reagents, plate and other requirements were prepared locally in our laboratory. Now it was applying successfully in allergy and immunology unit Ain Shams university hospitals in Egypt.
文摘Six grades of biopolymers formulated to have oil content of 40% (M1), 50% (M2), and 60% (M3) melon seed oil (MESO) and 40% (C1), 50% (C2), and 60% (C3) cottonseed oil (COSO) respectively, were prepared with phthalic anhydride, and glycerol using alcoholysis-polycondensation process. The extend of polycondensation was monitored by determining the acid value of aliquots of the reaction mixture at various intervals of time. Molecular weight averages and polydispersity index (PDI) of the finished alkyds were determined by Rast method and end-group analysis. Molecular weight averages and PDI vary with differences in oil length of the alkyds, with samples M2 and C2 respectively exhibiting the highest PDI. Molecular weight average obtained from end-group analysis and those determined by Rast method in brackets are 1338.92 (597.00), 982.33 (696.25), 1316.09 (754.03), and 1160.57 (448.13), 765.96 (583.57), 1049.92 (696.25) for samples M1, M2, M3 and C1, C2, C3 respectively. Number molecular weight averages calculated from end-group analysis are larger than those obtained by Rast method for both MESO and COSO alkyds and seem to grossly overestimate their molecular weights. The mode of variation of these properties indicates that the synthesis of MESO and COSO alkyds are complex. Correlation of PDI with the quality of the finished alkyds shows that the higher the PDI value the better the quality of the alkyd. Performance properties such as rate of drying, film hardness and resistance to chemicals were optimum at 50% oil length for both triglyceride oil alkyds.
基金This research work was financially assisted by University Grants Commission,Government of India,New Delhi,in the form of Junior and Senior Research Fellowship to Suman(Grant No.09/013(0617)/2016-EMR-I).
文摘In the present study,Paenibacillus lautus strain BHU3 isolated from landfill soil was evaluated for the presence of potential endoglucanases which are the first candidate of cellulase enzyme system to act on cellulose.In-silico analysis revealed high potential thermostable endoglucanases which can efficiently interact with cellulose.The most potent and thermostable endoglucanase(locus tag id.CPZ30_18280)belonged to glycosyl family-5 and had interaction energy of−12.981 kcal/mol for the best docked cluster containing three out of ten docking conformations,and Tm value of 73.3℃.MD simulation of 100 ns proved highly stable binding interactions of CPZ30_18280 endoglucanase with cellulose with root mean square deviation(RMSD)values ranging from 0.15 to 0.30 nm.Consistent interactions with characteristic active site residues(tyrosine,tryptophan and aspartate)of glycosyl family-5 endoglucanases were found.Further,to enhance the production of endoglucanases,the fermentation conditions were optimized employing approaches like one factor at a time(OFAT)and response surface methodology(RSM).Maximum activity of endoglucanase was determined at 60℃.The optimized condi-tions for enhanced production of endoglucanase(10.15 U/mL)were pH 6.63,yeast extract conc.3.44 g/L,wheat bran 3.59%,and inoculum size 2.65%.Hence,P.lautus strain BHU3 has enormous potential to synthesize highly efficient thermostable endoglucanases under optimized regime using agro-wastes.Thus,it could find immense industrial applications including large scale cellulose conversion to bioethanol.
文摘作者在原制备Der f Ⅰ变应原基础上进行其免疫化学特征鉴定。Der fⅠ的交叉免疫电泳(CIE)结果证实提纯的Der f Ⅰ为均质性。Der f Ⅰ氨基酸组成与Dandeu(1982)、Heymann(1986)报道的Der f Ⅰ相应结果相关性比较表明,三者在氨基酸组成上有相似性。采用放射性变应原吸附试验(RAST)检测55份螨过敏性患者血清抗Der f ⅠIgE抗体水平,其中49.09％病人RAST阳性,平均阳性特异性IgE水平为6.58±3.90百分结合率。结果表明Der f Ⅰ是粉尘螨主要的变应原。
文摘The heat transfer and flow characteristics of air jet impingement on a curved surface are investigated with computational fluid dynamics(CFD)approach.The first applied model is a one-equation SGS model for large eddy simulation(LES)and the second one is the SST-SAS hybrid RANS-LES.These models are utilized to study the flow physics in impinging process on a curved surface for different jet-to-surface(h/B)distances at two Reynolds numbers namely,2960 and 4740 based on the jet exit velocity(U_e)and the hydraulic diameter(2B).The predictions are compared with the experimental data in the literature and also the results from RANS k-εmodel.Comparisons show that both models can produce relatively good results.However,one-equation model(OEM)produced more accurate results especially at impingement region at lower jet-to-surface distances.In terms of heat transfer,the OEM also predicted better at different jet-to-surface spacings.It is also observed that both models show similar performance at higher h/B ratios.
