The detection and exploitation of naturally occurring DNA sequencepolymorphisms represent one of the most significant developments in molecular biology.Restriction endonuclease digestion of total genomic DNA followed ...The detection and exploitation of naturally occurring DNA sequencepolymorphisms represent one of the most significant developments in molecular biology.Restriction endonuclease digestion of total genomic DNA followed by hybridizationwith a radioactively labelled probe reveals differently sized hybridizing fragments. Thisform of polymorphism termed RFLP has been used extensively for genetic studies.However, the technique is not so efficient in detecting polymorphisms of展开更多
文摘The detection and exploitation of naturally occurring DNA sequencepolymorphisms represent one of the most significant developments in molecular biology.Restriction endonuclease digestion of total genomic DNA followed by hybridizationwith a radioactively labelled probe reveals differently sized hybridizing fragments. Thisform of polymorphism termed RFLP has been used extensively for genetic studies.However, the technique is not so efficient in detecting polymorphisms of
