Objective: To study the effect of Taoren Quyu Decoction (TQD) on endometrial cells in patients with endometriosis (EMs) and EMs in rats. Methods: A total of 60 female Wistar rats were randomly divided into 4 groups, n...Objective: To study the effect of Taoren Quyu Decoction (TQD) on endometrial cells in patients with endometriosis (EMs) and EMs in rats. Methods: A total of 60 female Wistar rats were randomly divided into 4 groups, namely, normal group, model group, positive group and TQD group, each group having 15 rats. Except the normal group, EMs model was established in the other three groups by transplanting the rat autologous endometrium. After 4 weeks of intragastric administration, blood, eutopic and ectopic endometrial tissues of rats in each group were collected to detect the serum levels of estrogen (E2), cancer antigen 125 (CA125), endometrial antibody (EMA13), and expressions of microvessel density (MVD), vascular endothelial growth factor (VEGF) and angiopoietin (Ang-2). The volume of endometriosis cyst was determined simultaneously. For the in vitro culture of human endometrial cells, 4 groups, namely, normal group, model group, positive group and TQD group were used. The positive group and TQD group were treated with danazol and TQD respectively. Then 24 h after the treatment, the expressions of survivin and tumor suppressor gene (p53) of each group were detected. Results: The volumes of the endometriosis cysts in the positive group and the TQD group were significantly reduced compared with the model group (P<0.05). The serum levels of E2, CA125 and EMAb, and the expressions of MVD. VEGF and Ang-2 in the model group were significantly increased compared with the normal group (P<0.0.5): while they were all significantly reduced in the positive group and TQD group (P<0.05). Compared with the normal group, the expression of survivin in the model group was significantly up regulated (/1/40.05), and expression of p53 was significantly reduced (P<0.05); compared with the model group, the expressions of survivin in the positive and TQD groups were significantly decreased (P<0.05), and expression of p53 was significantly up-regulated (P<0.05). The difference between positive group and TQD group was not statistically sig展开更多
目的:探讨祛瘀消肿汤加减联合仙灵骨葆胶囊对桡骨远端骨折患者术后生活质量的影响。方法:选择我院87例桡骨远端骨折患者,均行常规切开复位内固定手术治疗,按照随机数字表法分组,对照组43例予以仙灵骨葆胶囊治疗,观察组44例予以仙灵骨葆...目的:探讨祛瘀消肿汤加减联合仙灵骨葆胶囊对桡骨远端骨折患者术后生活质量的影响。方法:选择我院87例桡骨远端骨折患者,均行常规切开复位内固定手术治疗,按照随机数字表法分组,对照组43例予以仙灵骨葆胶囊治疗,观察组44例予以仙灵骨葆胶囊+祛瘀消肿汤加减治疗,观察比较两组疼痛消失时间、消肿时间、骨折愈合时间、视觉模拟(Visual analogue scale,VAS)评分及腕关节功能恢复、关节活动度变化情况,并统计两组不良反应发生情况及生活质量(The quality of life score,QOLS)评分。结果:观察组消肿、疼痛消失及骨折愈合时间、VAS评分均小于对照组,差异具有统计学意义(P<0.05);观察组腕关节功能恢复优良率为88.64%(39/44),高于对照组68.44%(29/43),差异具有统计学意义(P<0.05);治疗4周后两组患者背伸、掌屈及旋前、旋后活动范围均变大,且观察组较对照组大,差异具有统计学意义(P<0.05);观察组不良反应发生率为9.09%(4/44),对照组为4.65%(2/43),组间比较差异无统计学意义(P>0.05);治疗4周后两组患者QOLS(健康与功能、亲缘与婚姻幸福、个人社会与社区承诺)评分及总分均升高,且观察组较对照组高,差异具有统计学意义(P<0.05)。结论:仙灵骨葆胶囊联合祛瘀消肿汤加减治疗可改善桡骨远端骨折患者术后腕关节功能、关节活动范围及生活质量,缩短骨折愈合及疼痛、肿胀消失时间,且安全性高。展开更多
基金supported by Guangdong Provincial Medical Research Fund Project(A2011345)
文摘Objective: To study the effect of Taoren Quyu Decoction (TQD) on endometrial cells in patients with endometriosis (EMs) and EMs in rats. Methods: A total of 60 female Wistar rats were randomly divided into 4 groups, namely, normal group, model group, positive group and TQD group, each group having 15 rats. Except the normal group, EMs model was established in the other three groups by transplanting the rat autologous endometrium. After 4 weeks of intragastric administration, blood, eutopic and ectopic endometrial tissues of rats in each group were collected to detect the serum levels of estrogen (E2), cancer antigen 125 (CA125), endometrial antibody (EMA13), and expressions of microvessel density (MVD), vascular endothelial growth factor (VEGF) and angiopoietin (Ang-2). The volume of endometriosis cyst was determined simultaneously. For the in vitro culture of human endometrial cells, 4 groups, namely, normal group, model group, positive group and TQD group were used. The positive group and TQD group were treated with danazol and TQD respectively. Then 24 h after the treatment, the expressions of survivin and tumor suppressor gene (p53) of each group were detected. Results: The volumes of the endometriosis cysts in the positive group and the TQD group were significantly reduced compared with the model group (P<0.05). The serum levels of E2, CA125 and EMAb, and the expressions of MVD. VEGF and Ang-2 in the model group were significantly increased compared with the normal group (P<0.0.5): while they were all significantly reduced in the positive group and TQD group (P<0.05). Compared with the normal group, the expression of survivin in the model group was significantly up regulated (/1/40.05), and expression of p53 was significantly reduced (P<0.05); compared with the model group, the expressions of survivin in the positive and TQD groups were significantly decreased (P<0.05), and expression of p53 was significantly up-regulated (P<0.05). The difference between positive group and TQD group was not statistically sig
文摘目的:探讨祛瘀消肿汤加减联合仙灵骨葆胶囊对桡骨远端骨折患者术后生活质量的影响。方法:选择我院87例桡骨远端骨折患者,均行常规切开复位内固定手术治疗,按照随机数字表法分组,对照组43例予以仙灵骨葆胶囊治疗,观察组44例予以仙灵骨葆胶囊+祛瘀消肿汤加减治疗,观察比较两组疼痛消失时间、消肿时间、骨折愈合时间、视觉模拟(Visual analogue scale,VAS)评分及腕关节功能恢复、关节活动度变化情况,并统计两组不良反应发生情况及生活质量(The quality of life score,QOLS)评分。结果:观察组消肿、疼痛消失及骨折愈合时间、VAS评分均小于对照组,差异具有统计学意义(P<0.05);观察组腕关节功能恢复优良率为88.64%(39/44),高于对照组68.44%(29/43),差异具有统计学意义(P<0.05);治疗4周后两组患者背伸、掌屈及旋前、旋后活动范围均变大,且观察组较对照组大,差异具有统计学意义(P<0.05);观察组不良反应发生率为9.09%(4/44),对照组为4.65%(2/43),组间比较差异无统计学意义(P>0.05);治疗4周后两组患者QOLS(健康与功能、亲缘与婚姻幸福、个人社会与社区承诺)评分及总分均升高,且观察组较对照组高,差异具有统计学意义(P<0.05)。结论:仙灵骨葆胶囊联合祛瘀消肿汤加减治疗可改善桡骨远端骨折患者术后腕关节功能、关节活动范围及生活质量,缩短骨折愈合及疼痛、肿胀消失时间,且安全性高。
