Summary: Whether surfactant protein B (SP-B)-18A/C and 1580C/T polymorphism were associated with susceptibility to chronic obstructive pulmonary disease (COPD) in Chinese Han population was investigated. After genomi...Summary: Whether surfactant protein B (SP-B)-18A/C and 1580C/T polymorphism were associated with susceptibility to chronic obstructive pulmonary disease (COPD) in Chinese Han population was investigated. After genomic DNA was isolated from blood of COPD smokers and control smokers, the genotypes of SP-B-18A/C and SP-B1580C/T polymorphism loci were determined by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) respectively. The results showed that there was significant difference in genotypes distribution frequency of SP-B1580C/T polymorphism locus between COPD smokers and control smokers. C→T mutation rate (including TT homozygote and CT heterozygote) in COPD smokers was higher than in control smokers (57.9 % vs 41.7 %, χ2=4.93, P<0.05), whereas there was no significant difference in genotypes distribution frequency of SP-B1580-18A/C locus between COPD smokers and control smokers. The allele frequency (29.1 %) of SP-B1580-18A/C locus is lower than T allele (70.9 %) in Chinese Han Population, and the distribution was different from that in Mexican, in which, the A and T allele frequencies were 85 % and 15 % respectively. It was concluded that SP-B1580 T allele was probably associated with increased susceptibility to COPD in Chinese Han population; The polymorphism of SP-B-18A/C locus maybe varied with race.展开更多
目的探索未成熟肺与成熟肺在重度冲击伤后同一时间点肺泡灌洗液中SP-B含量差异,伤后相关指标变化、特点及意义。方法 4周龄幼年健康新西兰白兔[幼年兔组,雌雄不拘40只,体质量(442.10±55.30)g]与24周龄成年健康新西兰白兔[成年兔组...目的探索未成熟肺与成熟肺在重度冲击伤后同一时间点肺泡灌洗液中SP-B含量差异,伤后相关指标变化、特点及意义。方法 4周龄幼年健康新西兰白兔[幼年兔组,雌雄不拘40只,体质量(442.10±55.30)g]与24周龄成年健康新西兰白兔[成年兔组,雌雄不拘40只,体质量(2018.15±145.80)g],采用不同驱动压构建实验动物伤情一致的动物模型(驱动压:幼年兔组4.5 MPa,成年兔组4.0 MPa)。在两组伤情一致情况下分别于伤即刻(0 h),2、4、6、12、24、48、72 h检测肺泡灌洗液中SP-B浓度(ELISA法)、观测动物肺大体解剖和光镜病理、测定肺组织含水量和生理指标等。结果两组肺损伤程度较为一致(P>0.05),所有时间点幼年兔组SP-B浓度均低于成年兔组(P<0.05)。幼年兔以肺泡壁断裂和损伤居多,成年兔以肺泡及间质内出血及炎症细胞浸润多见。伤后2 h肺含水率(82.68±0.58 vs 81.06±0.34)、伤后0.5 h血氧饱和度(75.00±18.52 vs 42.20±21.84)均为幼年兔高于成年兔(P<0.05)。伤后6 h(36.92±16.01 vs 60.30±12.09)、12 h(31.42±11.30 vs 46.40±14.15)、24 h(29.48±4.76 vs 35.52±9.77)和48 h(25.70±11.66 vs 37.68±9.11)中性粒细胞百分比则为幼年兔组低于成年兔组(P<0.05)。结论冲击伤后幼年兔未成熟肺与成年兔成熟肺相比肺水肿恢复较快、炎症反应较轻;但SP-B含量较低、合成和分泌功能恢复较慢,可能导致其呼吸功能恢复较慢;从而用以指导不同成熟度肺损伤的临床救治。展开更多
文摘Summary: Whether surfactant protein B (SP-B)-18A/C and 1580C/T polymorphism were associated with susceptibility to chronic obstructive pulmonary disease (COPD) in Chinese Han population was investigated. After genomic DNA was isolated from blood of COPD smokers and control smokers, the genotypes of SP-B-18A/C and SP-B1580C/T polymorphism loci were determined by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) respectively. The results showed that there was significant difference in genotypes distribution frequency of SP-B1580C/T polymorphism locus between COPD smokers and control smokers. C→T mutation rate (including TT homozygote and CT heterozygote) in COPD smokers was higher than in control smokers (57.9 % vs 41.7 %, χ2=4.93, P<0.05), whereas there was no significant difference in genotypes distribution frequency of SP-B1580-18A/C locus between COPD smokers and control smokers. The allele frequency (29.1 %) of SP-B1580-18A/C locus is lower than T allele (70.9 %) in Chinese Han Population, and the distribution was different from that in Mexican, in which, the A and T allele frequencies were 85 % and 15 % respectively. It was concluded that SP-B1580 T allele was probably associated with increased susceptibility to COPD in Chinese Han population; The polymorphism of SP-B-18A/C locus maybe varied with race.
文摘目的观察脂多糖(lipopolysaccharide,LPS)诱导急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)大鼠的肺表面活性蛋白B(surfactant proteins B,SP-B)、纤溶酶原活化抑制因子(plasminogen activator inhibitor-1,PAI-1)、高迁移率族蛋白B1(high-mobility group box B1,HMGB1)和可溶性晚期糖基化终产物受体(soluble receptor for advanced glycation end products,sRAGE)等四项生物标志物的早期变化特点,为临床联合检测提供依据。方法实验在中山大学心肺脑复苏研究所完成,36只雄性SD大鼠随机(随机数字法)分成LPS组和对照组,每组18只;LPS组采用肺内注射LPS 10μg/mL诱导建立ARDS模型,对照组则肺内注射等量生理盐水,在造模后6 h、12 h、24 h留取血清、肺泡灌洗液及肺组织,每个时间点各6只大鼠,动脉血气及病理分析确认建模成功后用ELISA、免疫组化及Western blot检测SP-B、PAI-1、HMGB1和sRAGE的表达变化。采用成组t检验和重复测量的方差分析比较两组间各指标的差异。结果LPS组PaO2(mmHg)随时间进行性降低,且与对照组比较差异有统计学意义(80.38±1.74 vs 84.17±1.96;72.20±1.70 vs 81.26±1.57;68.52±2.45 vs 81.45±1.51,P<0.05),HE染色提示肺损伤程度持续恶化。LPS组血清中HMGB1(ng/mL)(0.34±0.08,0.43±0.12,0.50±0.03),SP-B(ng/mL)(2.21±0.11,2.74±0.15,3.07±0.28)和PAI-1(ng/mL)(40.38±3.06,50.02±4.82,57.34±4.67)进行性升高,与对照组比较差异统计学意义(均P<0.05),但sRAGE(ng/mL)与对照组相比进行性下降(0.17±0.04,0.14±0.04,0.11±0.02),仅24 h与其他时点差异有统计学意义(P<0.05)。LPS组肺泡灌洗液中HMGB1(ng/mL)(0.08±0.02,0.13±0.02,0.21±0.03)、PAI-1(ng/mL)(48.88±4.17,56.66±4.01,65.83±4.96)和SP-B(ng/mL)(0.68±0.13,0.80±0.17,1.12±0.14)进行性升高;而sRAGE(ng/mL)进行性下降(0.14±0.05,0.12±0.02,0.11±0.02),且与对照组比较差异有统计学意义(P<0.05)。免疫组化分析提示6 h起PAI-1在肺间质逐步上调表达而HMGB1和SP-B则呈现
文摘目的探索未成熟肺与成熟肺在重度冲击伤后同一时间点肺泡灌洗液中SP-B含量差异,伤后相关指标变化、特点及意义。方法 4周龄幼年健康新西兰白兔[幼年兔组,雌雄不拘40只,体质量(442.10±55.30)g]与24周龄成年健康新西兰白兔[成年兔组,雌雄不拘40只,体质量(2018.15±145.80)g],采用不同驱动压构建实验动物伤情一致的动物模型(驱动压:幼年兔组4.5 MPa,成年兔组4.0 MPa)。在两组伤情一致情况下分别于伤即刻(0 h),2、4、6、12、24、48、72 h检测肺泡灌洗液中SP-B浓度(ELISA法)、观测动物肺大体解剖和光镜病理、测定肺组织含水量和生理指标等。结果两组肺损伤程度较为一致(P>0.05),所有时间点幼年兔组SP-B浓度均低于成年兔组(P<0.05)。幼年兔以肺泡壁断裂和损伤居多,成年兔以肺泡及间质内出血及炎症细胞浸润多见。伤后2 h肺含水率(82.68±0.58 vs 81.06±0.34)、伤后0.5 h血氧饱和度(75.00±18.52 vs 42.20±21.84)均为幼年兔高于成年兔(P<0.05)。伤后6 h(36.92±16.01 vs 60.30±12.09)、12 h(31.42±11.30 vs 46.40±14.15)、24 h(29.48±4.76 vs 35.52±9.77)和48 h(25.70±11.66 vs 37.68±9.11)中性粒细胞百分比则为幼年兔组低于成年兔组(P<0.05)。结论冲击伤后幼年兔未成熟肺与成年兔成熟肺相比肺水肿恢复较快、炎症反应较轻;但SP-B含量较低、合成和分泌功能恢复较慢,可能导致其呼吸功能恢复较慢;从而用以指导不同成熟度肺损伤的临床救治。
