The cell suspension cultures in leaves of Pueraria lobata (Willd.) Ohwi seedlings were treated with salicylic acid (SA), methyl jasmonate (MJ) and ethrel respectively. SA and MJ could cause cell brown, while ethre...The cell suspension cultures in leaves of Pueraria lobata (Willd.) Ohwi seedlings were treated with salicylic acid (SA), methyl jasmonate (MJ) and ethrel respectively. SA and MJ could cause cell brown, while ethrel had a positive effect on cell vitality. 0.1 mg/L SA and 1.0 mg/L MJ could promote the synthesis of puerarin and hence improved the yield. Ethrel, SA and MJ at higher concentrations restrained the product of puerarin. All these three substances had no effects on increasing cell biomass and puerarin release.展开更多
The contents of flavonoids and puerarin in Pueraria lobata(Willd.) Ohwi were measured by UV-spectrophotometry and HPLC.The results showed that contents of flavonoids and puerarin in P.lobata were related to different ...The contents of flavonoids and puerarin in Pueraria lobata(Willd.) Ohwi were measured by UV-spectrophotometry and HPLC.The results showed that contents of flavonoids and puerarin in P.lobata were related to different parts and gathering season.The flavonoids and puerarin content from vines gathered in autumn and winter were the highest,reached to 5.18%-6.12% and 0.982%-1.718% respectively.The vine is regarded as the available medicinal resources and is worth exploiting.展开更多
文摘The cell suspension cultures in leaves of Pueraria lobata (Willd.) Ohwi seedlings were treated with salicylic acid (SA), methyl jasmonate (MJ) and ethrel respectively. SA and MJ could cause cell brown, while ethrel had a positive effect on cell vitality. 0.1 mg/L SA and 1.0 mg/L MJ could promote the synthesis of puerarin and hence improved the yield. Ethrel, SA and MJ at higher concentrations restrained the product of puerarin. All these three substances had no effects on increasing cell biomass and puerarin release.
文摘The contents of flavonoids and puerarin in Pueraria lobata(Willd.) Ohwi were measured by UV-spectrophotometry and HPLC.The results showed that contents of flavonoids and puerarin in P.lobata were related to different parts and gathering season.The flavonoids and puerarin content from vines gathered in autumn and winter were the highest,reached to 5.18%-6.12% and 0.982%-1.718% respectively.The vine is regarded as the available medicinal resources and is worth exploiting.
