Background:Hepatocellular carcinoma(HCC)has a high incidence and mortality that epitomizes one of the prominent causes of cancer-related death globally.Novel therapeutic approaches are therefore required.Reactive oxyg...Background:Hepatocellular carcinoma(HCC)has a high incidence and mortality that epitomizes one of the prominent causes of cancer-related death globally.Novel therapeutic approaches are therefore required.Reactive oxygen species(ROS)are necessary for maintaining cell cycle.Although ROS is involved in HCC progression,hydrogen peroxide(H2O2)has anti-proliferative effect on HCC.Method:HCC Huh-7 cells were cultured and incubated with various concentrations of H2O2.Paraoxonase activity,levels of malondialdehyde,glutathione and protein oxidation were measured in treated and untreated Huh-7 cells.Furthermore,untreated and treated Huh-7 cells were subjected to two dimensional gel electrophoresis and identified protein spots which were differentially expressed by LC-MS/MS analysis.q RT-PCR was performed to validate the identified proteins.Results:H2O2 depleted glutathione(GSH)with the concomitant up-regulation of GSTP1 and Prx2.H2O2 also increased malondialdehyde and protein oxidation,decreased the activity of paraoxonase in Huh-7 cells.Conclusion:H2O2 could be used as a novel therapeutic agent that might be beneficial in inducing cell cytotoxicity and hence suppress HCC proliferation.展开更多
基金supported by a grant from the Higher Education Commission,Islamabad-Pakistan(NRPU 20-4386)。
文摘Background:Hepatocellular carcinoma(HCC)has a high incidence and mortality that epitomizes one of the prominent causes of cancer-related death globally.Novel therapeutic approaches are therefore required.Reactive oxygen species(ROS)are necessary for maintaining cell cycle.Although ROS is involved in HCC progression,hydrogen peroxide(H2O2)has anti-proliferative effect on HCC.Method:HCC Huh-7 cells were cultured and incubated with various concentrations of H2O2.Paraoxonase activity,levels of malondialdehyde,glutathione and protein oxidation were measured in treated and untreated Huh-7 cells.Furthermore,untreated and treated Huh-7 cells were subjected to two dimensional gel electrophoresis and identified protein spots which were differentially expressed by LC-MS/MS analysis.q RT-PCR was performed to validate the identified proteins.Results:H2O2 depleted glutathione(GSH)with the concomitant up-regulation of GSTP1 and Prx2.H2O2 also increased malondialdehyde and protein oxidation,decreased the activity of paraoxonase in Huh-7 cells.Conclusion:H2O2 could be used as a novel therapeutic agent that might be beneficial in inducing cell cytotoxicity and hence suppress HCC proliferation.
