In this research, a surface imprinting strategy has been adopted in protein imprinting. Bovine hemo-globin surface-imprinted polystyrene (PS) nanoparticles with magnetic susceptibility have been syn-thesized through m...In this research, a surface imprinting strategy has been adopted in protein imprinting. Bovine hemo-globin surface-imprinted polystyrene (PS) nanoparticles with magnetic susceptibility have been syn-thesized through multistage core-shell polymerization system using 3-aminophenylboronic acid (APBA) as functional and cross-linking monomers. Superparamagnetic molecularly imprinted polystyrene nanospheres with poly(APBA) thin films have been synthesized and used for the first time for protein molecular imprinting in an aqueous solution. The magnetic susceptibility is imparted through the successful encapsulation of Fe3O4 nanoparticles. The morphology, adsorption, and recognition prop-erties of superparamagnetic molecularly imprinted polymers (MIPs) have been investigated using transmission electron microscopy, X-ray diffraction, thermogravimetric analysis, and vibrating sample magnetometer. Rebinding experimental results show that poly(APBA) MIPs-coated superparamagnetic PS nanoparticles have high adsorption capacity for template protein bovine hemoglobin and compara-tively low nonspecific adsorption. The imprinted superparamagnetic nanoparticles could easily reach the adsorption equilibrium and achieve magnetic separation in an external magnetic field, thus avoid-ing some problems of the bulk polymer.展开更多
Background:This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pel eted prod...Background:This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pel eted products(BPP)based on co-products(canola or carinata)from processing with different proportions of pulse pea screenings and lignosulfonate chemical compound.Method:The protein molecular structures were determined using the non-invasive advanced vibrational molecular spectroscopy(ATR-FT/IR)in terms of chemical structure and biofunctional groups of amides(ⅠandⅡ),α-helix andβ-sheet.Results:The results showed that increasing the level of the co-products in BPP significantly increased the spectral intensity of the amide area and amide height.The products exhibited similar protein secondaryα-helix toβ-sheet ratio.The protein molecular structure profiles(amidesⅠandⅡ,α-helix toβ-sheet)were highly associated with protein degradation kinetics and intestinal digestion.In conclusion,the non-invasive vibrational molecular spectroscopy(ATR-FT/IR)could be used to detect inherent structural make-up characteristics in BPP.Conclusion:The molecular structural features related to protein biopolymer were highly associated with protein utilization and metabolism.展开更多
近年来,随着核桃产量和核桃深加工发展,核桃油成为加工热点。大量核桃粕因含有核桃内果皮而被废弃或作为饲料,造成蛋白资源浪费。试验对液压冷榨后的核桃粕基本组成、蛋白质氨基酸构成及其分子量进行研究,以期对脱脂核桃蛋白进行合理利...近年来,随着核桃产量和核桃深加工发展,核桃油成为加工热点。大量核桃粕因含有核桃内果皮而被废弃或作为饲料,造成蛋白资源浪费。试验对液压冷榨后的核桃粕基本组成、蛋白质氨基酸构成及其分子量进行研究,以期对脱脂核桃蛋白进行合理利用,使其成为人类蛋白资源。核桃粕成分分析表明,蛋白质、脂肪、水分、灰分及碳水化合物含量依次为50.20%,24.67%,7.82%,5.80%及11.51%;氨基酸分析发现,其含有16种氨基酸,包含6种人体必需氨基酸,含量最高的氨基酸是谷氨酸,为70.17 mg/g,其次是精氨酸,为62.24 mg/g,酪氨酸含量最低,为0.41 mg/g。核桃粕核桃蛋白质分子量分布研究表明,核桃蛋白质分子亚基约14条,分布范围在7.8~97.4 k Da,主要集中于23.49k Da和37.41 k Da。展开更多
基金Supported by the National High-Tech Research & Development Program of China (Grant No. 2007AA10Z432)the National Basic Research Program (Grant No. 2007CB914100)+1 种基金the National Natural Science Foundation of China (Grant Nos. 20675040 & 20875050)the Natural Science Foundation of Tianjin (Grant No. 07JCYBJC00500)
文摘In this research, a surface imprinting strategy has been adopted in protein imprinting. Bovine hemo-globin surface-imprinted polystyrene (PS) nanoparticles with magnetic susceptibility have been syn-thesized through multistage core-shell polymerization system using 3-aminophenylboronic acid (APBA) as functional and cross-linking monomers. Superparamagnetic molecularly imprinted polystyrene nanospheres with poly(APBA) thin films have been synthesized and used for the first time for protein molecular imprinting in an aqueous solution. The magnetic susceptibility is imparted through the successful encapsulation of Fe3O4 nanoparticles. The morphology, adsorption, and recognition prop-erties of superparamagnetic molecularly imprinted polymers (MIPs) have been investigated using transmission electron microscopy, X-ray diffraction, thermogravimetric analysis, and vibrating sample magnetometer. Rebinding experimental results show that poly(APBA) MIPs-coated superparamagnetic PS nanoparticles have high adsorption capacity for template protein bovine hemoglobin and compara-tively low nonspecific adsorption. The imprinted superparamagnetic nanoparticles could easily reach the adsorption equilibrium and achieve magnetic separation in an external magnetic field, thus avoid-ing some problems of the bulk polymer.
基金financially supported by the grants from Sask Pulse Growers,Natural Sciences and Engineering Research Council of Canada(NSERC)the Sask Canola,the Ministry of Agriculture Strategic Research Chair ProgramSask Milk.
文摘Background:This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pel eted products(BPP)based on co-products(canola or carinata)from processing with different proportions of pulse pea screenings and lignosulfonate chemical compound.Method:The protein molecular structures were determined using the non-invasive advanced vibrational molecular spectroscopy(ATR-FT/IR)in terms of chemical structure and biofunctional groups of amides(ⅠandⅡ),α-helix andβ-sheet.Results:The results showed that increasing the level of the co-products in BPP significantly increased the spectral intensity of the amide area and amide height.The products exhibited similar protein secondaryα-helix toβ-sheet ratio.The protein molecular structure profiles(amidesⅠandⅡ,α-helix toβ-sheet)were highly associated with protein degradation kinetics and intestinal digestion.In conclusion,the non-invasive vibrational molecular spectroscopy(ATR-FT/IR)could be used to detect inherent structural make-up characteristics in BPP.Conclusion:The molecular structural features related to protein biopolymer were highly associated with protein utilization and metabolism.
文摘近年来,随着核桃产量和核桃深加工发展,核桃油成为加工热点。大量核桃粕因含有核桃内果皮而被废弃或作为饲料,造成蛋白资源浪费。试验对液压冷榨后的核桃粕基本组成、蛋白质氨基酸构成及其分子量进行研究,以期对脱脂核桃蛋白进行合理利用,使其成为人类蛋白资源。核桃粕成分分析表明,蛋白质、脂肪、水分、灰分及碳水化合物含量依次为50.20%,24.67%,7.82%,5.80%及11.51%;氨基酸分析发现,其含有16种氨基酸,包含6种人体必需氨基酸,含量最高的氨基酸是谷氨酸,为70.17 mg/g,其次是精氨酸,为62.24 mg/g,酪氨酸含量最低,为0.41 mg/g。核桃粕核桃蛋白质分子量分布研究表明,核桃蛋白质分子亚基约14条,分布范围在7.8~97.4 k Da,主要集中于23.49k Da和37.41 k Da。
