目的:建立盐酸普萘洛尔凝胶的微生物限度检查方法。方法:利用氯化钙能与凝胶中的壳聚糖结合形成钙盐沉淀达到破胶目的的原理,用10%氯化钙溶液10 m L与盐酸普萘洛尔凝胶10 g混匀,加0.9%无菌氯化钠溶液至100 m L,得1∶10破胶供试液,取1 m ...目的:建立盐酸普萘洛尔凝胶的微生物限度检查方法。方法:利用氯化钙能与凝胶中的壳聚糖结合形成钙盐沉淀达到破胶目的的原理,用10%氯化钙溶液10 m L与盐酸普萘洛尔凝胶10 g混匀,加0.9%无菌氯化钠溶液至100 m L,得1∶10破胶供试液,取1 m L加0.9%无菌氯化钠溶液100 m L稀释后过膜,用300 m L 0.9%无菌氯化钠溶液分3次冲洗滤膜,每次100 m L,边冲洗边振摇,泵速为160 r/min。结果:经验证试验,采用上述方法加菌回收率均大于70%,盐酸普萘洛尔凝胶在此实验条件下无抑菌作用或抑菌作用弱。结论:盐酸普萘洛尔凝胶中的细菌、霉菌、酵母菌的检查可采用薄膜过滤法,控制菌检查可采用常规法,结果可靠。展开更多
The effects of l-, d- and dl-propranolol on antipyrine metabolism and isoprenaline-induced tachycardia were studied in rabbits. The plasma concentration of antipyrine was assayed by Brodie’s spectrophotometric method...The effects of l-, d- and dl-propranolol on antipyrine metabolism and isoprenaline-induced tachycardia were studied in rabbits. The plasma concentration of antipyrine was assayed by Brodie’s spectrophotometric method and the change of heart rate was evaluated by ECG. Both isomers of propranolol hydrochloride were administered iv （800 μg/kg） to the rabbits. d-propranolol produced an antipyrine t 1/2 of 2.67±0.77h and a cl of 0.49±0.1 l/h while its levo-form produced an antipyrine t 1/2 of 1.7±0.39h and a cl of 0.88±0.22 l/h. The differences between the half-lives and between the clearances were statistically significant （P【0.05 for t 1/2, P【0.01 for cl）. The inhibitory effects of l- and d-propranolol on isoprenaline-induced tachycardia were assessed. l-propranolol yielded an ID<sub>50</sub> which was only 1/88 of d-propranolol’s. Our studies showed that the β-receptor blocking activity of propranolol mainly came. from its levoform while the effect on antipyrine metabolism came from its dextro-form.展开更多
文摘目的:建立盐酸普萘洛尔凝胶的微生物限度检查方法。方法:利用氯化钙能与凝胶中的壳聚糖结合形成钙盐沉淀达到破胶目的的原理,用10%氯化钙溶液10 m L与盐酸普萘洛尔凝胶10 g混匀,加0.9%无菌氯化钠溶液至100 m L,得1∶10破胶供试液,取1 m L加0.9%无菌氯化钠溶液100 m L稀释后过膜,用300 m L 0.9%无菌氯化钠溶液分3次冲洗滤膜,每次100 m L,边冲洗边振摇,泵速为160 r/min。结果:经验证试验,采用上述方法加菌回收率均大于70%,盐酸普萘洛尔凝胶在此实验条件下无抑菌作用或抑菌作用弱。结论:盐酸普萘洛尔凝胶中的细菌、霉菌、酵母菌的检查可采用薄膜过滤法,控制菌检查可采用常规法,结果可靠。
文摘The effects of l-, d- and dl-propranolol on antipyrine metabolism and isoprenaline-induced tachycardia were studied in rabbits. The plasma concentration of antipyrine was assayed by Brodie’s spectrophotometric method and the change of heart rate was evaluated by ECG. Both isomers of propranolol hydrochloride were administered iv （800 μg/kg） to the rabbits. d-propranolol produced an antipyrine t 1/2 of 2.67±0.77h and a cl of 0.49±0.1 l/h while its levo-form produced an antipyrine t 1/2 of 1.7±0.39h and a cl of 0.88±0.22 l/h. The differences between the half-lives and between the clearances were statistically significant （P【0.05 for t 1/2, P【0.01 for cl）. The inhibitory effects of l- and d-propranolol on isoprenaline-induced tachycardia were assessed. l-propranolol yielded an ID<sub>50</sub> which was only 1/88 of d-propranolol’s. Our studies showed that the β-receptor blocking activity of propranolol mainly came. from its levoform while the effect on antipyrine metabolism came from its dextro-form.
