AIM： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. METHODS： HepG2 cell line was tested for its susceptibility to HCV by i...AIM： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. METHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.展开更多
Nonlinear effect is of importance to waves propagating from deep water to shallow water. The non-linearity of waves is widely discussed due to its high precision in application. But there are still some problems in de...Nonlinear effect is of importance to waves propagating from deep water to shallow water. The non-linearity of waves is widely discussed due to its high precision in application. But there are still some problems in dealing with the nonlinear waves in practice. In this paper, a modified form of mild-slope equation with weakly nonlinear effect is derived by use of the nonlinear dispersion relation and the steady mild-slope equation containing energy dissipation. The modified form of mild-slope equation is convenient to solve nonlinear effect of waves. The model is tested against the laboratory measurement for the case of a submerged elliptical shoal on a slope beach given by Berkhoff et al. The present numerical results are also compared with those obtained through linear wave theory. Better agreement is obtained as the modified mild-slope equation is employed. And the modified mild-slope equation can reasonably simulate the weakly nonlinear effect of wave propagation from deep water to coast.展开更多
基金Supported by the Ministry of Scientific Research, Academy of Scientific Research and Technology, Medical Research Council Code: P5-MED-030-01 and US-Egypt joint project BIO7-002-011
文摘AIM： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. METHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.
文摘Nonlinear effect is of importance to waves propagating from deep water to shallow water. The non-linearity of waves is widely discussed due to its high precision in application. But there are still some problems in dealing with the nonlinear waves in practice. In this paper, a modified form of mild-slope equation with weakly nonlinear effect is derived by use of the nonlinear dispersion relation and the steady mild-slope equation containing energy dissipation. The modified form of mild-slope equation is convenient to solve nonlinear effect of waves. The model is tested against the laboratory measurement for the case of a submerged elliptical shoal on a slope beach given by Berkhoff et al. The present numerical results are also compared with those obtained through linear wave theory. Better agreement is obtained as the modified mild-slope equation is employed. And the modified mild-slope equation can reasonably simulate the weakly nonlinear effect of wave propagation from deep water to coast.
