为揭示糙皮侧耳原基发育的潜在调控物质,采用代谢组学的方法对糙皮侧耳菌丝体和原基细胞代谢物进行检测分析。结果表明,菌丝体与原基细胞的代谢物具有显著差异。采用正交偏最小二乘判别分析(OPLS‐DA),以VIP(varible importance in the ...为揭示糙皮侧耳原基发育的潜在调控物质,采用代谢组学的方法对糙皮侧耳菌丝体和原基细胞代谢物进行检测分析。结果表明,菌丝体与原基细胞的代谢物具有显著差异。采用正交偏最小二乘判别分析(OPLS‐DA),以VIP(varible importance in the projection)≥1和倍数变化≥2或≤0.5为条件共筛选到400种差异代谢物。调控通路分析表明,这些差异物质涉及33条代谢通路,其中丙氨酸、天冬氨酸和谷氨酸、氨酰tRNA生物合成、嘌呤代谢等8条代谢通路具有极显著影响。谷氨酸和谷氨酰胺涉及p<0.01的大部分代谢通路,可能在糙皮侧耳原基发育过程中具有重要调控作用。研究结果为糙皮侧耳以及其他大型真菌原基发育机制的探索提供了理论依据。展开更多
Diclinism is not only an interesting natural phenomenon in plant kingdom, but also of significance in the yield of crops, e.g. cucumber and watermelon. Cucumber has been a model plant for studying the diclinism for de...Diclinism is not only an interesting natural phenomenon in plant kingdom, but also of significance in the yield of crops, e.g. cucumber and watermelon. Cucumber has been a model plant for studying the diclinism for decades. However, it is stili obscure how the carpel primordia of cucumber male flower undergo the developmental processes after the initiation of their development. By showing morphological alternation, metabolic activity, as well as CFL gene expression, the present study demonstrated that the carpel primordia of cucumber male flowers maintained the characteristics of primordium until anthesis, although it developed significantly slower than that of the female flowers.展开更多
Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We comb...Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction （RAP.PCR） and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice, We believe that the findings described here will help to elucidate the molecular mechanism（s） underlying the developmental processes of rice leaf展开更多
文摘为揭示糙皮侧耳原基发育的潜在调控物质,采用代谢组学的方法对糙皮侧耳菌丝体和原基细胞代谢物进行检测分析。结果表明,菌丝体与原基细胞的代谢物具有显著差异。采用正交偏最小二乘判别分析(OPLS‐DA),以VIP(varible importance in the projection)≥1和倍数变化≥2或≤0.5为条件共筛选到400种差异代谢物。调控通路分析表明,这些差异物质涉及33条代谢通路,其中丙氨酸、天冬氨酸和谷氨酸、氨酰tRNA生物合成、嘌呤代谢等8条代谢通路具有极显著影响。谷氨酸和谷氨酰胺涉及p<0.01的大部分代谢通路,可能在糙皮侧耳原基发育过程中具有重要调控作用。研究结果为糙皮侧耳以及其他大型真菌原基发育机制的探索提供了理论依据。
文摘Diclinism is not only an interesting natural phenomenon in plant kingdom, but also of significance in the yield of crops, e.g. cucumber and watermelon. Cucumber has been a model plant for studying the diclinism for decades. However, it is stili obscure how the carpel primordia of cucumber male flower undergo the developmental processes after the initiation of their development. By showing morphological alternation, metabolic activity, as well as CFL gene expression, the present study demonstrated that the carpel primordia of cucumber male flowers maintained the characteristics of primordium until anthesis, although it developed significantly slower than that of the female flowers.
基金Supported by the Area of Excellence Grant on Plant and Fungal Biotechnologyfrom the University Grants Committee of the Hong Kong SAR Government
文摘Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction （RAP.PCR） and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice, We believe that the findings described here will help to elucidate the molecular mechanism（s） underlying the developmental processes of rice leaf
