目的探讨空腹血糖水平对合并2型糖尿病的中老年高血压患者血压昼夜节律的影响。方法选取2017年1月—2019年10月首都医科大学宣武医院综合科原发性高血压住院患者311例,根据有无2型糖尿病分为对照组(n=151)及观察组(n=160),比较2组血糖...目的探讨空腹血糖水平对合并2型糖尿病的中老年高血压患者血压昼夜节律的影响。方法选取2017年1月—2019年10月首都医科大学宣武医院综合科原发性高血压住院患者311例,根据有无2型糖尿病分为对照组(n=151)及观察组(n=160),比较2组血糖、血脂水平,进行24 h动态血压监测,分析空腹血糖对血压昼夜节律的影响。结果观察组空腹血糖(FPG)、糖化血红蛋白(HbA1c)高于对照组,24小时收缩压(24 h SBP)、日间收缩压(DSBP)、夜间收缩压(NSBP)高于对照组,NSBP下降率低于对照组(t/P=82.908/0.000、4.921/0.027、9.633/0.002、7.392/0.007、12.583/0.000、6.682/0.010),2组的昼夜节律构成比例不同,差异有统计学意义(χ2/P=6.765/0.034)。在杓型组、非杓型组及反杓型组中,空腹血糖水平及糖尿病比例逐渐升高(t/P=6.904/0.001,χ2/P=6.765/0.034)。Logistic回归分析发现,空腹血糖水平升高是昼夜节律异常的危险因素[OR(95%CI)=1.157(1.016~1.318),P=0.028]。结论合并2型糖尿病中老年高血压患者发生血压昼夜节律异常的风险高于未合并2型糖尿病患者,空腹血糖水平升高是其发生血压昼夜节律异常的危险因素。展开更多
Grazing is a main human activity in the grasslands of Xinjiang, China. It is vital to identify the effects of grazing on the sustainable utilization of local grasslands. However, the effects of grazing on net primary ...Grazing is a main human activity in the grasslands of Xinjiang, China. It is vital to identify the effects of grazing on the sustainable utilization of local grasslands. However, the effects of grazing on net primary productivity （NPP）, evapotranspiration （ET） and water use efficiency （WUE） in this region remain unclear. Using the spatial Biome-BGC grazing model, we explored the effects of grazing on NPP, ET and WUE across the different regions and grassland types in Xinjiang during 1979-2012. NPP, ET and WUE under the grazed scenario were generally lower than those under the ungrazed scenario, and the differences showed increasing trends over time. The decreases in NPP, ET and WUE varied significantly among the regions and grassland types. NPP decreased as follows： among the regions, Northern Xinjiang （16.60 g C/（m2·a））, Tianshan Mountains （15.94 g C/（m2·a）） and Southern Xinjiang （-3.54 g C/（m2·a））; and among the grassland types, typical grasslands （25.70 g C/（m2·a））, swamp meadows （25.26 g C/（m2·a））, mid-mountain meadows （23.39 g C/（m2·a））, alpine meadows （6.33 g C/（m2·a））, desert grasslands （5.82 g C/（m2·a）） and saline meadows （2.90 g C/（me.a））. ET decreased as follows： among the regions, Tianshan Mountains （28.95 mm/a）, Northern Xinjiang （8.11 mm/a） and Southern Xinjiang （7.57 mm/a）; and among the grassland types, mid-mountain meadows （29.30 mm/a）, swamp meadows （25.07 mm·a）, typical grasslands （24.56 mm/a）, alpine meadows （20.69 mm/a）, desert grasslands （11.06 mm/a） and saline meadows （3.44 mm/a）. WUE decreased as follows： among the regions, Northern Xinjiang （0.053 g C/kg H2O）, Tianshan Mountains （0.034 g C/kg H2O） and Southern Xinjiang （0.012 g C/kg H2O）; and among the grassland types, typical grasslands （0.0609 g C/kg H2O）, swamp meadows （0.0548 g C/kg H2O）, mid-mountain meadows （0.0501 g C/kg H2O）, desert grasslands （0.0172 g C/kg H2O）, alpine 展开更多
Objective To observe the therapeutic effect of moxibustion on primary dysmenorrhea due to damp- cold retention. Methods Eighty cases with primary dysmenorrhea due to damp-cold retention were randomly divided into a mo...Objective To observe the therapeutic effect of moxibustion on primary dysmenorrhea due to damp- cold retention. Methods Eighty cases with primary dysmenorrhea due to damp-cold retention were randomly divided into a moxibustion group （40 cases） and a medication group （40 cases）. Moxibustion at Guonyudn （关元 CV 4） and Shiqizhui （十七椎 EX-B 8） was adopted for treatment of the moxibustion group a week before the period, which lasted for 10 days as a treatment course. Fenbid was used as oral administration for three continue menstrual periods for the medication group. The treatment lasted for 3 menstrual periods in two groups. The Cox Menstrual Symptom Scale （CMSS） was adopted to grade the menstrual symptoms, and differences between the two groups were compared. Results The total effective rate of the moxibustion group was 97.5% （39/40）, and the total effective rate of the medication group was 72.5% （29/40）. The effect of the moxibustion group was obviously better than the medication group （P〈0.05）. Before and after treatment the menstrual symptom scores of the moxibustion group were 9.78±1.86 and 2.25±3.33, while the medication group were 9.71±1.64 and 5.31±4.26. The scores of both groups decreased obviously after treatments. And the decreased amplitude of the moxibustion group was much more obvious than that of the medication group （P〈0.05）. Conclusion The effect of moxibustion on primary dysmenorrhea due to damp-cold retention is obvious, which is better than Fenbid.展开更多
Great volumes of shallow-buried （〈2,000 m） natural gases which are mainly composed of biogases and low-mature gases have been found in the Mesozoic-Cenozoic sedimentary basins in China. Many shallow gas reservoirs ...Great volumes of shallow-buried （〈2,000 m） natural gases which are mainly composed of biogases and low-mature gases have been found in the Mesozoic-Cenozoic sedimentary basins in China. Many shallow gas reservoirs in China are characterized by coexistence of biogas and low-mature gas, so identifying the genetic types of shallow gases is important for exploration and development in sedimentary basins. In this paper, we study the gas geochemistry characteristics and distribution in different basins, and classify the shallow gas into two genetic types, biogas and low-mature gas. The biogases are subdivided further into two subtypes by their sources, the source rock-derived biogas and hydrocarbon-derived biogas. Based on the burial history of the source rocks, the source rock-derived biogases are divided into primary and secondary biogas. The former is generated from the source rocks in the primary burial stage, and the latter is from uplifted source rocks or those in a secondary burial stage. In addition, the identifying parameters of each type of shallow gas are given. Based on the analysis above, the distributions of each type of shallow gas are studied. The primary biogases generated from source rocks are mostly distributed in Quaternary basins or modem deltas. Most of them migrate in watersoluble or diffused mode, and their migration distance is short. Reservoir and caprock assemblages play an important role in primary biogas accumulation. The secondary biogases are distributed in a basin with secondary burial history. The oil-degraded biogases are distributed near heavy oil pools. The low-mature gases are widely distributed in shallow-buried reservoirs in the Meso-Cenozoic basins.展开更多
Chronic systemic inflammation in obesity-associated type 2 diabetes (T2D) is a key inducing factor of insulin resistance (IR). Hydrogen molecule (H2) has been proved to be a safe and effective anti-inflammatory agent,...Chronic systemic inflammation in obesity-associated type 2 diabetes (T2D) is a key inducing factor of insulin resistance (IR). Hydrogen molecule (H2) has been proved to be a safe and effective anti-inflammatory agent, but conventional H2 administration methods cannot provide a high dosage and a long duration of H2 treatment in IR-related tissues and thus lead to limited therapeutic efficacies. We here propose a new strategy of controlled H2 release to match the time window of gastric emptying for maximizing the bioavailability and therapeutic outcome of H2. This work enhances the hydrolysis rate of Zn by constructing a Zn-Fe primary-battery micro-/ nano-structure, and the H2-releasing rate is adjusted by tuning the ratio of Zn to Fe. The Zn-Fe micro-/nano-structure is orally administrated once daily to alleviate obesity-associated T2D in a leptin-deficient (ob/ob) mouse model. The H2 generation time of the Zn-Fe primary-battery micro-/nano-structure with the Fe/Zn ratio of 1:100 in gastric acid is about 3 h, just matching with the time window of gastric emptying in mice. In vivo monitoring results show that H2 generated by Zn-Fe micro-/nano-structure in stomach can effectively accumulate in major IR-sited tissues including liver, adipose tissue, and skeletal muscle at a high dose for a relatively long time compared to H2-rich water drinking. Oral administration of Zn-Fe micro-/nano-structure at 200 mg/kg body weight has realized an efficient IR improvement and remarkably ameliorated systemic inflammation in ob/ ob mice. In addition, a high-dose administration of Zn-Fe shows no visible toxicity in mice. This work provides a new strategy to maximize the outcome of hydrogen therapy.展开更多
Objective: By optimizing the extreme learning machine network with particle swarm optimization, we established a syndrome classification and prediction model for primary liver cancer(PLC), classified and predicted the...Objective: By optimizing the extreme learning machine network with particle swarm optimization, we established a syndrome classification and prediction model for primary liver cancer(PLC), classified and predicted the syndrome diagnosis of medical record data for PLC and compared and analyzed the prediction results with different algorithms and the clinical diagnosis results. This paper provides modern technical support for clinical diagnosis and treatment, and improves the objectivity, accuracy and rigor of the classification of traditional Chinese medicine(TCM) syndromes.Methods: From three top-level TCM hospitals in Nanchang, 10,602 electronic medical records from patients with PLC were collected, dating from January 2009 to May 2020. We removed the electronic medical records of 542 cases of syndromes and adopted the cross-validation method in the remaining10,060 electronic medical records, which were randomly divided into a training set and a test set.Based on fuzzy mathematics theory, we quantified the syndrome-related factors of TCM symptoms and signs, and information from the TCM four diagnostic methods. Next, using an extreme learning machine network with particle swarm optimization, we constructed a neural network syndrome classification and prediction model that used "TCM symptoms + signs + tongue diagnosis information + pulse diagnosis information" as input, and PLC syndrome as output. This approach was used to mine the nonlinear relationship between clinical data in electronic medical records and different syndrome types. The accuracy rate of classification was used to compare this model to other machine learning classification models.Results: The classification accuracy rate of the model developed here was 86.26%. The classification accuracy rates of models using support vector machine and Bayesian networks were 82.79% and 85.84%,respectively. The classification accuracy rates of the models for all syndromes in this paper were between82.15% and 93.82%.Conclusion: Compared with the case of data proces展开更多
Primary hyperoxaluria type 1(PH1)is a rare but devastating autosomal recessive inherited disease caused by mutations in gene AGXT.Pathogenic mutations of AGXT were mostly reported in Caucasian but infrequently in Asia...Primary hyperoxaluria type 1(PH1)is a rare but devastating autosomal recessive inherited disease caused by mutations in gene AGXT.Pathogenic mutations of AGXT were mostly reported in Caucasian but infrequently in Asian,especially in Chinese.To update the genotypes of PH1 in the Chinese population,we collected and identified 7 Chinese probands with PH1 from 2013 to 2017 in our center,five of whom had delayed diagnosis and failed in kidney transplantation.Samples of peripheral blood DNA from the 7 patients and their family members were collected and sequencing analysis was performed to test the mutations of gene AGXT.Western blotting and enzyme activity analysis were conducted to evaluate the function of the mutations.Furthermore,a systematic review from 1998 to 2017 was performed to observe the genetic characteristics between Chinese and Caucasian. The results showed that a total of 12 mutations were identified in the 7 pedigrees.To the best of ourknowledge,2 novel variants of A GXT,p.Gly41 Trp and p.Leu33Met,were first reported.Bioinformatics and functional analysis showed that only 7 mutations led to a reduced expression of alanine-glyoxylate amino transferase (AGT)at a protein level.The systematic review revealed significant population heterogeneity in PH1.In conclusion,new genetic subtypes and genetic characteristics of PH1 are updated in the Chinese population. Furthermore,a genotype-phenotype correlation is found in PH1.展开更多
Objective This work explores the impact of electroacupuncture(EA)on acute postoperative pain(APP)and the role of stimulator of interferon genes/type-1 interferon(STING/IFN-1)signaling pathway modulation in the analges...Objective This work explores the impact of electroacupuncture(EA)on acute postoperative pain(APP)and the role of stimulator of interferon genes/type-1 interferon(STING/IFN-1)signaling pathway modulation in the analgesic effect of EA in APP rats.Methods The APP rat model was initiated through abdominal surgery and the animals received two 30 min sessions of EA at bilateral ST36(Zusanli)and SP6(Sanyinjiao)acupoints.Mechanical,thermal and cold sensitivity tests were performed to measure the pain threshold,and electroencephalograms were recorded in the primary somatosensory cortex to identify the effects of EA treatment on APP.Western blotting and immunofluorescence were used to examine the expression and distribution of proteins in the STING/IFN-1 pathway as well as neuroinflammation.A STING inhibitor(C-176)was administered intrathecally to verify its role in EA.Results APP rats displayed mechanical and thermal hypersensitivities compared to the control group(P<0.05).APP significantly reduced the amplitude ofθ,αandγoscillations compared to their baseline values(P<0.05).Interestingly,expression levels of proteins in the STING/IFN-1 pathway were downregulated after inducing APP(P<0.05).Further,APP increased pro-inflammatory factors,including interleukin-6,tumor necrosis factor-αand inducible nitric oxide synthase,and downregulated anti-inflammatory factors,including interleukin-10 and arginase-1(P<0.05).EA effectively attenuated APP-induced painful hypersensitivities(P<0.05)and restored theθ,αandγpower in APP rats(P<0.05).Meanwhile,EA distinctly activated the STING/IFN-1 pathway and mitigated the neuroinflammatory response(P<0.05).Furthermore,STING/IFN-1 was predominantly expressed in isolectin-B4-or calcitonin-gene-related-peptide-labeled dorsal root ganglion neurons and superficial laminae of the spinal dorsal horn.Inhibition of the STING/IFN-1 pathway by intrathecal injection of C-176 weakened the analgesic and anti-inflammatory effects of EA on APP(P<0.05).Conclusion EA can generate robust analgesic and ant展开更多
文摘目的探讨空腹血糖水平对合并2型糖尿病的中老年高血压患者血压昼夜节律的影响。方法选取2017年1月—2019年10月首都医科大学宣武医院综合科原发性高血压住院患者311例,根据有无2型糖尿病分为对照组(n=151)及观察组(n=160),比较2组血糖、血脂水平,进行24 h动态血压监测,分析空腹血糖对血压昼夜节律的影响。结果观察组空腹血糖(FPG)、糖化血红蛋白(HbA1c)高于对照组,24小时收缩压(24 h SBP)、日间收缩压(DSBP)、夜间收缩压(NSBP)高于对照组,NSBP下降率低于对照组(t/P=82.908/0.000、4.921/0.027、9.633/0.002、7.392/0.007、12.583/0.000、6.682/0.010),2组的昼夜节律构成比例不同,差异有统计学意义(χ2/P=6.765/0.034)。在杓型组、非杓型组及反杓型组中,空腹血糖水平及糖尿病比例逐渐升高(t/P=6.904/0.001,χ2/P=6.765/0.034)。Logistic回归分析发现,空腹血糖水平升高是昼夜节律异常的危险因素[OR(95%CI)=1.157(1.016~1.318),P=0.028]。结论合并2型糖尿病中老年高血压患者发生血压昼夜节律异常的风险高于未合并2型糖尿病患者,空腹血糖水平升高是其发生血压昼夜节律异常的危险因素。
基金supported financially by the National Natural Science Foundation of China(41361140361,41271126)the Project of State Key Laboratory of Desert and Oasis Ecology(Y471163)
文摘Grazing is a main human activity in the grasslands of Xinjiang, China. It is vital to identify the effects of grazing on the sustainable utilization of local grasslands. However, the effects of grazing on net primary productivity （NPP）, evapotranspiration （ET） and water use efficiency （WUE） in this region remain unclear. Using the spatial Biome-BGC grazing model, we explored the effects of grazing on NPP, ET and WUE across the different regions and grassland types in Xinjiang during 1979-2012. NPP, ET and WUE under the grazed scenario were generally lower than those under the ungrazed scenario, and the differences showed increasing trends over time. The decreases in NPP, ET and WUE varied significantly among the regions and grassland types. NPP decreased as follows： among the regions, Northern Xinjiang （16.60 g C/（m2·a））, Tianshan Mountains （15.94 g C/（m2·a）） and Southern Xinjiang （-3.54 g C/（m2·a））; and among the grassland types, typical grasslands （25.70 g C/（m2·a））, swamp meadows （25.26 g C/（m2·a））, mid-mountain meadows （23.39 g C/（m2·a））, alpine meadows （6.33 g C/（m2·a））, desert grasslands （5.82 g C/（m2·a）） and saline meadows （2.90 g C/（me.a））. ET decreased as follows： among the regions, Tianshan Mountains （28.95 mm/a）, Northern Xinjiang （8.11 mm/a） and Southern Xinjiang （7.57 mm/a）; and among the grassland types, mid-mountain meadows （29.30 mm/a）, swamp meadows （25.07 mm·a）, typical grasslands （24.56 mm/a）, alpine meadows （20.69 mm/a）, desert grasslands （11.06 mm/a） and saline meadows （3.44 mm/a）. WUE decreased as follows： among the regions, Northern Xinjiang （0.053 g C/kg H2O）, Tianshan Mountains （0.034 g C/kg H2O） and Southern Xinjiang （0.012 g C/kg H2O）; and among the grassland types, typical grasslands （0.0609 g C/kg H2O）, swamp meadows （0.0548 g C/kg H2O）, mid-mountain meadows （0.0501 g C/kg H2O）, desert grasslands （0.0172 g C/kg H2O）, alpine
文摘Objective To observe the therapeutic effect of moxibustion on primary dysmenorrhea due to damp- cold retention. Methods Eighty cases with primary dysmenorrhea due to damp-cold retention were randomly divided into a moxibustion group （40 cases） and a medication group （40 cases）. Moxibustion at Guonyudn （关元 CV 4） and Shiqizhui （十七椎 EX-B 8） was adopted for treatment of the moxibustion group a week before the period, which lasted for 10 days as a treatment course. Fenbid was used as oral administration for three continue menstrual periods for the medication group. The treatment lasted for 3 menstrual periods in two groups. The Cox Menstrual Symptom Scale （CMSS） was adopted to grade the menstrual symptoms, and differences between the two groups were compared. Results The total effective rate of the moxibustion group was 97.5% （39/40）, and the total effective rate of the medication group was 72.5% （29/40）. The effect of the moxibustion group was obviously better than the medication group （P〈0.05）. Before and after treatment the menstrual symptom scores of the moxibustion group were 9.78±1.86 and 2.25±3.33, while the medication group were 9.71±1.64 and 5.31±4.26. The scores of both groups decreased obviously after treatments. And the decreased amplitude of the moxibustion group was much more obvious than that of the medication group （P〈0.05）. Conclusion The effect of moxibustion on primary dysmenorrhea due to damp-cold retention is obvious, which is better than Fenbid.
文摘Great volumes of shallow-buried （〈2,000 m） natural gases which are mainly composed of biogases and low-mature gases have been found in the Mesozoic-Cenozoic sedimentary basins in China. Many shallow gas reservoirs in China are characterized by coexistence of biogas and low-mature gas, so identifying the genetic types of shallow gases is important for exploration and development in sedimentary basins. In this paper, we study the gas geochemistry characteristics and distribution in different basins, and classify the shallow gas into two genetic types, biogas and low-mature gas. The biogases are subdivided further into two subtypes by their sources, the source rock-derived biogas and hydrocarbon-derived biogas. Based on the burial history of the source rocks, the source rock-derived biogases are divided into primary and secondary biogas. The former is generated from the source rocks in the primary burial stage, and the latter is from uplifted source rocks or those in a secondary burial stage. In addition, the identifying parameters of each type of shallow gas are given. Based on the analysis above, the distributions of each type of shallow gas are studied. The primary biogases generated from source rocks are mostly distributed in Quaternary basins or modem deltas. Most of them migrate in watersoluble or diffused mode, and their migration distance is short. Reservoir and caprock assemblages play an important role in primary biogas accumulation. The secondary biogases are distributed in a basin with secondary burial history. The oil-degraded biogases are distributed near heavy oil pools. The low-mature gases are widely distributed in shallow-buried reservoirs in the Meso-Cenozoic basins.
基金supported by the National Natural Science Foundation of China[82172078,81770855,82200508]Academic Promotion Programme of Shandong First Medical University[2019QL010]+1 种基金National Key Research and Development Program of China[2022YFB3804500]Shenzhen Science and Technology Program[RCJC20210706092010008].
文摘Chronic systemic inflammation in obesity-associated type 2 diabetes (T2D) is a key inducing factor of insulin resistance (IR). Hydrogen molecule (H2) has been proved to be a safe and effective anti-inflammatory agent, but conventional H2 administration methods cannot provide a high dosage and a long duration of H2 treatment in IR-related tissues and thus lead to limited therapeutic efficacies. We here propose a new strategy of controlled H2 release to match the time window of gastric emptying for maximizing the bioavailability and therapeutic outcome of H2. This work enhances the hydrolysis rate of Zn by constructing a Zn-Fe primary-battery micro-/ nano-structure, and the H2-releasing rate is adjusted by tuning the ratio of Zn to Fe. The Zn-Fe micro-/nano-structure is orally administrated once daily to alleviate obesity-associated T2D in a leptin-deficient (ob/ob) mouse model. The H2 generation time of the Zn-Fe primary-battery micro-/nano-structure with the Fe/Zn ratio of 1:100 in gastric acid is about 3 h, just matching with the time window of gastric emptying in mice. In vivo monitoring results show that H2 generated by Zn-Fe micro-/nano-structure in stomach can effectively accumulate in major IR-sited tissues including liver, adipose tissue, and skeletal muscle at a high dose for a relatively long time compared to H2-rich water drinking. Oral administration of Zn-Fe micro-/nano-structure at 200 mg/kg body weight has realized an efficient IR improvement and remarkably ameliorated systemic inflammation in ob/ ob mice. In addition, a high-dose administration of Zn-Fe shows no visible toxicity in mice. This work provides a new strategy to maximize the outcome of hydrogen therapy.
基金financially supported by the National Natural Science Foundation (No. 81660727)。
文摘Objective: By optimizing the extreme learning machine network with particle swarm optimization, we established a syndrome classification and prediction model for primary liver cancer(PLC), classified and predicted the syndrome diagnosis of medical record data for PLC and compared and analyzed the prediction results with different algorithms and the clinical diagnosis results. This paper provides modern technical support for clinical diagnosis and treatment, and improves the objectivity, accuracy and rigor of the classification of traditional Chinese medicine(TCM) syndromes.Methods: From three top-level TCM hospitals in Nanchang, 10,602 electronic medical records from patients with PLC were collected, dating from January 2009 to May 2020. We removed the electronic medical records of 542 cases of syndromes and adopted the cross-validation method in the remaining10,060 electronic medical records, which were randomly divided into a training set and a test set.Based on fuzzy mathematics theory, we quantified the syndrome-related factors of TCM symptoms and signs, and information from the TCM four diagnostic methods. Next, using an extreme learning machine network with particle swarm optimization, we constructed a neural network syndrome classification and prediction model that used "TCM symptoms + signs + tongue diagnosis information + pulse diagnosis information" as input, and PLC syndrome as output. This approach was used to mine the nonlinear relationship between clinical data in electronic medical records and different syndrome types. The accuracy rate of classification was used to compare this model to other machine learning classification models.Results: The classification accuracy rate of the model developed here was 86.26%. The classification accuracy rates of models using support vector machine and Bayesian networks were 82.79% and 85.84%,respectively. The classification accuracy rates of the models for all syndromes in this paper were between82.15% and 93.82%.Conclusion: Compared with the case of data proces
基金This work was supported by the grants from the Special Project of Ministry of Health (No.201302009)and the National Natural Science Foundation of China (No. 81700300).
文摘Primary hyperoxaluria type 1(PH1)is a rare but devastating autosomal recessive inherited disease caused by mutations in gene AGXT.Pathogenic mutations of AGXT were mostly reported in Caucasian but infrequently in Asian,especially in Chinese.To update the genotypes of PH1 in the Chinese population,we collected and identified 7 Chinese probands with PH1 from 2013 to 2017 in our center,five of whom had delayed diagnosis and failed in kidney transplantation.Samples of peripheral blood DNA from the 7 patients and their family members were collected and sequencing analysis was performed to test the mutations of gene AGXT.Western blotting and enzyme activity analysis were conducted to evaluate the function of the mutations.Furthermore,a systematic review from 1998 to 2017 was performed to observe the genetic characteristics between Chinese and Caucasian. The results showed that a total of 12 mutations were identified in the 7 pedigrees.To the best of ourknowledge,2 novel variants of A GXT,p.Gly41 Trp and p.Leu33Met,were first reported.Bioinformatics and functional analysis showed that only 7 mutations led to a reduced expression of alanine-glyoxylate amino transferase (AGT)at a protein level.The systematic review revealed significant population heterogeneity in PH1.In conclusion,new genetic subtypes and genetic characteristics of PH1 are updated in the Chinese population. Furthermore,a genotype-phenotype correlation is found in PH1.
基金This work was supported by the National Natural Science Foundation of China(Grant No.82071251)National Key Research and Development Program of China(Grant No.2018YFC2001802)Hubei Province Key Research and Development Program(Grant No.2021BCA145).
文摘Objective This work explores the impact of electroacupuncture(EA)on acute postoperative pain(APP)and the role of stimulator of interferon genes/type-1 interferon(STING/IFN-1)signaling pathway modulation in the analgesic effect of EA in APP rats.Methods The APP rat model was initiated through abdominal surgery and the animals received two 30 min sessions of EA at bilateral ST36(Zusanli)and SP6(Sanyinjiao)acupoints.Mechanical,thermal and cold sensitivity tests were performed to measure the pain threshold,and electroencephalograms were recorded in the primary somatosensory cortex to identify the effects of EA treatment on APP.Western blotting and immunofluorescence were used to examine the expression and distribution of proteins in the STING/IFN-1 pathway as well as neuroinflammation.A STING inhibitor(C-176)was administered intrathecally to verify its role in EA.Results APP rats displayed mechanical and thermal hypersensitivities compared to the control group(P<0.05).APP significantly reduced the amplitude ofθ,αandγoscillations compared to their baseline values(P<0.05).Interestingly,expression levels of proteins in the STING/IFN-1 pathway were downregulated after inducing APP(P<0.05).Further,APP increased pro-inflammatory factors,including interleukin-6,tumor necrosis factor-αand inducible nitric oxide synthase,and downregulated anti-inflammatory factors,including interleukin-10 and arginase-1(P<0.05).EA effectively attenuated APP-induced painful hypersensitivities(P<0.05)and restored theθ,αandγpower in APP rats(P<0.05).Meanwhile,EA distinctly activated the STING/IFN-1 pathway and mitigated the neuroinflammatory response(P<0.05).Furthermore,STING/IFN-1 was predominantly expressed in isolectin-B4-or calcitonin-gene-related-peptide-labeled dorsal root ganglion neurons and superficial laminae of the spinal dorsal horn.Inhibition of the STING/IFN-1 pathway by intrathecal injection of C-176 weakened the analgesic and anti-inflammatory effects of EA on APP(P<0.05).Conclusion EA can generate robust analgesic and ant
