Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 （ANGPTL3） can contribute to disorder of carbohydrate and lipid metabolism. The presen...Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 （ANGPTL3） can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia, We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d ofrefeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.展开更多
本研究旨在评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)对围产期母羊能量负平衡及其产羔性能的影响。选取80头湖羊,随机分为4组,每组20头,对照组饲喂基础饲粮,RPG组、RPC组和RPC+RPG组每头每天分别添加15 g RPG、3 g RPC、15 g RPG+3 g RP...本研究旨在评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)对围产期母羊能量负平衡及其产羔性能的影响。选取80头湖羊,随机分为4组,每组20头,对照组饲喂基础饲粮,RPG组、RPC组和RPC+RPG组每头每天分别添加15 g RPG、3 g RPC、15 g RPG+3 g RPC。试验期50 d,结果表明:1)各组间妊娠毒血症的发病率无显著差异(P>0.05),但与对照组相比,试验组有提高羔羊断奶成活率的趋势(0.10>P>0.05),且RPC+RPG组羔羊初生重显著提高(P<0.05)。2)与对照组相比,添加RPG显著降低了分娩当天母羊血清中β-羟基丁酸含量(P<0.05),同时血清中总蛋白含量也显著提高(P<0.05),还显著提高了产后10 d时血清甲状腺素(T4)含量(P<0.05),对血清葡萄糖含量影响不显著(P>0.05)。3)与对照组相比,添加RPC对血清葡萄糖和β-羟基丁酸含量影响均不显著(P>0.05),相反还显著提高了分娩当天和产后20 d的血清谷丙转氨酶活性(P<0.05),添加RPC+RPG对血清生化指标无显著交互作用(P>0.05)。综上所述,围产期母羊饲粮中添加15 g/(d•头)RPG有助于缓解能量负平衡,而3 g/(d•头)RPC添加效果不显著,但添加RPC+RPG均可以提升羔羊断奶成活率,提高羔羊初生重。展开更多
基金the National Natural Science Foundation of China (31072167)the Key Project of Hunan Provincial Science & Technology Department, China(2009FJ1005)Innovation Project of Hunan Provincial Education Department (CX2009B153), China
文摘Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 （ANGPTL3） can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia, We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d ofrefeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.
文摘本研究旨在评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)对围产期母羊能量负平衡及其产羔性能的影响。选取80头湖羊,随机分为4组,每组20头,对照组饲喂基础饲粮,RPG组、RPC组和RPC+RPG组每头每天分别添加15 g RPG、3 g RPC、15 g RPG+3 g RPC。试验期50 d,结果表明:1)各组间妊娠毒血症的发病率无显著差异(P>0.05),但与对照组相比,试验组有提高羔羊断奶成活率的趋势(0.10>P>0.05),且RPC+RPG组羔羊初生重显著提高(P<0.05)。2)与对照组相比,添加RPG显著降低了分娩当天母羊血清中β-羟基丁酸含量(P<0.05),同时血清中总蛋白含量也显著提高(P<0.05),还显著提高了产后10 d时血清甲状腺素(T4)含量(P<0.05),对血清葡萄糖含量影响不显著(P>0.05)。3)与对照组相比,添加RPC对血清葡萄糖和β-羟基丁酸含量影响均不显著(P>0.05),相反还显著提高了分娩当天和产后20 d的血清谷丙转氨酶活性(P<0.05),添加RPC+RPG对血清生化指标无显著交互作用(P>0.05)。综上所述,围产期母羊饲粮中添加15 g/(d•头)RPG有助于缓解能量负平衡,而3 g/(d•头)RPC添加效果不显著,但添加RPC+RPG均可以提升羔羊断奶成活率,提高羔羊初生重。
