A nonlinear synthesis problem of antennas according to the prescribed power (squared amplitude) radiation pattern (RP) is considered in the variational statement that yields in the possibility to take into account an ...A nonlinear synthesis problem of antennas according to the prescribed power (squared amplitude) radiation pattern (RP) is considered in the variational statement that yields in the possibility to take into account an additional restriction to the synthesized power RP. The problem of synthesis consists of finding such currents in antenna, which generates the RP with the best approximation to the given one. The respective Euler’s equation is reduced on the basis of used functional. This is nonlinear integral equation of Hammerstein’s type. The effective numerical methods are elaborated and applied for its solving. The computational results verify the effectiveness of approach proposed.展开更多
[目的]以6份不同地理来源的糜子资源为试验材料,基于前期转录组测序获得的1000对SSR引物,选出200对进行多态性检测,以期构建一批可以准确评估糜子种质遗传差异的分子标记.[方法]用Primer Premier 5.0软件设计引物,改良CTAB法提取DNA,PC...[目的]以6份不同地理来源的糜子资源为试验材料,基于前期转录组测序获得的1000对SSR引物,选出200对进行多态性检测,以期构建一批可以准确评估糜子种质遗传差异的分子标记.[方法]用Primer Premier 5.0软件设计引物,改良CTAB法提取DNA,PCR扩增DNA和聚丙烯酰胺凝胶电泳筛选引物多态性;用PowerMarker 3.25和PopGen 1.32计算遗传多样性参数.[结果]200对引物中97对呈单态性,80对呈多态性.单碱基序列重复引物有20对,10对具多态性,其重复基元是A(50%)和T(50%).二碱基序列重复引物有36对,15对具多态性,其碱基重复类型有7种(AG最多,TC、GC和GA次之,CA、TA和AC最少).三碱基序列重复引物有144对,55对具多态性,其碱基重复类型有24种(GGC、GCG和GCC最多,GAA、GCT和CGC等次之,ACC、AGG、CAG、CGT、AAG、AAC、TCG、CGA、ATT、CAA和CCA最少).就引物分辨率(Rp)值而言,1-3碱基序列重复引物分别为0.67-4.67(平均2.07)、1.33-4.33(平均2.73)和0.67-4.00(平均1.83).基于Rp值分析SSR分布频次,发现80个标记分布在5个区间:0-1、1-2、2-3、3-4和4-5,分别包含17(21.25%)、36(45.00%)、11(13.75%)、14(17.50%)和2个(2.50%).就Rp值而言,1-3碱基序列重复引物分别为0.33-0.67(平均0.51)、0.40-0.78(平均0.59)和0.33-0.83(平均0.59).单碱基序列重复标记共检测到22个等位变异,每个位点为2-3个(平均2.2000),其中8个和2个位点分别具2和3个变异;二碱基序列重复标记共检测到38个等位变异,每个位点为2-3个(平均2.5333),其中7个和8个位点分别具2和3个变异;三碱基重复标记共检测到136个等位变异,每个位点为2-3个(平均2.4727),其中29个和26个位点分别具2和3个变异.就多态性信息含量而言,1-3碱基序列重复引物分别为0.3750-0.5355(平均0.4293)、0.2392-0.7438(平均0.4293)和0.2392-0.7438(平均0.3989).就多样性指数而言,1-3碱基序列重复分别为0.6365-1.0776(平均0.7497)、0.5623-1.0986(平均0.8339)和0.5623-1.0889(平均0.83展开更多
文摘A nonlinear synthesis problem of antennas according to the prescribed power (squared amplitude) radiation pattern (RP) is considered in the variational statement that yields in the possibility to take into account an additional restriction to the synthesized power RP. The problem of synthesis consists of finding such currents in antenna, which generates the RP with the best approximation to the given one. The respective Euler’s equation is reduced on the basis of used functional. This is nonlinear integral equation of Hammerstein’s type. The effective numerical methods are elaborated and applied for its solving. The computational results verify the effectiveness of approach proposed.
文摘[目的]以6份不同地理来源的糜子资源为试验材料,基于前期转录组测序获得的1000对SSR引物,选出200对进行多态性检测,以期构建一批可以准确评估糜子种质遗传差异的分子标记.[方法]用Primer Premier 5.0软件设计引物,改良CTAB法提取DNA,PCR扩增DNA和聚丙烯酰胺凝胶电泳筛选引物多态性;用PowerMarker 3.25和PopGen 1.32计算遗传多样性参数.[结果]200对引物中97对呈单态性,80对呈多态性.单碱基序列重复引物有20对,10对具多态性,其重复基元是A(50%)和T(50%).二碱基序列重复引物有36对,15对具多态性,其碱基重复类型有7种(AG最多,TC、GC和GA次之,CA、TA和AC最少).三碱基序列重复引物有144对,55对具多态性,其碱基重复类型有24种(GGC、GCG和GCC最多,GAA、GCT和CGC等次之,ACC、AGG、CAG、CGT、AAG、AAC、TCG、CGA、ATT、CAA和CCA最少).就引物分辨率(Rp)值而言,1-3碱基序列重复引物分别为0.67-4.67(平均2.07)、1.33-4.33(平均2.73)和0.67-4.00(平均1.83).基于Rp值分析SSR分布频次,发现80个标记分布在5个区间:0-1、1-2、2-3、3-4和4-5,分别包含17(21.25%)、36(45.00%)、11(13.75%)、14(17.50%)和2个(2.50%).就Rp值而言,1-3碱基序列重复引物分别为0.33-0.67(平均0.51)、0.40-0.78(平均0.59)和0.33-0.83(平均0.59).单碱基序列重复标记共检测到22个等位变异,每个位点为2-3个(平均2.2000),其中8个和2个位点分别具2和3个变异;二碱基序列重复标记共检测到38个等位变异,每个位点为2-3个(平均2.5333),其中7个和8个位点分别具2和3个变异;三碱基重复标记共检测到136个等位变异,每个位点为2-3个(平均2.4727),其中29个和26个位点分别具2和3个变异.就多态性信息含量而言,1-3碱基序列重复引物分别为0.3750-0.5355(平均0.4293)、0.2392-0.7438(平均0.4293)和0.2392-0.7438(平均0.3989).就多样性指数而言,1-3碱基序列重复分别为0.6365-1.0776(平均0.7497)、0.5623-1.0986(平均0.8339)和0.5623-1.0889(平均0.83
