为了探究茯苓配方(茯苓、山楂、黄大茶)浸膏对高脂饮食小鼠肥胖及脂质沉积的影响,将C57/BL6N小鼠随机分为对照组,模型组,奥利司他药物对照组,茯苓配方(Poria cocos Wolf formula,PWF)浸膏低、高剂量组。除对照组给予低脂饲料,其他组均...为了探究茯苓配方(茯苓、山楂、黄大茶)浸膏对高脂饮食小鼠肥胖及脂质沉积的影响,将C57/BL6N小鼠随机分为对照组,模型组,奥利司他药物对照组,茯苓配方(Poria cocos Wolf formula,PWF)浸膏低、高剂量组。除对照组给予低脂饲料,其他组均以高脂饲料喂养12周。每周记录小鼠的体重、能量摄入。给药结束后,计算各组小鼠Lee’s指数、脏器系数,采用苏木精-伊红(hematoxylin-eosin,HE)染色法观察各组小鼠肝细胞与脂肪细胞形态变化,全自动生化仪测定各组小鼠血清中TG、TC、HDL-C、LDL-C、AST及ALT水平变化。结果显示,与对照组相比,模型组小鼠体重、Lee’s指数、脏器系数、TG、TC、LDL-C、AST及ALT水平均显著升高(P<0.01或P<0.001),肝脏脂滴数量与脂肪细胞直径增加;与模型组相比,茯苓配方浸膏高剂量组小鼠体重、Lee’s指数和脏器系数均显著降低(P<0.05或P<0.01),低剂量组小鼠体重、Lee’s指数和脏器系数均没有显著变化,茯苓配方浸膏高剂量组小鼠肝脏脂滴数量与脂肪细胞直径减少,TG、TC、LDL-C水平、AST及ALT水平均显著降低(P<0.05或P<0.01或P<0.001)。综上所述,茯苓配方浸膏能够改善高脂饮食小鼠的体重及脂质沉积,为开发改善肥胖的天然物质提供理论基础。展开更多
Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effec...Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effects on recurrent spontaneous abortion and that can maintain pregnancy until delivery. It was hypothesized that this herbal medicine can also prolong allograft survival after organ transplantation. Here,in an in vivo study,we report the anti-rejection effect of the ethanol extract of Poria cocos Wolf (EEPCW) in rats after cardiac allograft implantation. Methods Ten normal rats were healthy controls. Eighty rats receiving homologous heart transplants were divided into 4 groups of 20 rats each based on type of treatment: olive oil 8 ml·kg -1 ·d -1 ,EEPCW 25 mg·kg -1 ·d -1 , EEPCW 50 mg·kg -1 ·d -1 or cyclosporin A 5mg·kg -1 ·d -1 . Allograft survival was observed in 10 rats from each group. On the seventh day post transplantation,pathological lesions and percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and the CD_4 +/CD_8 + ratio in peripheral blood were assessed in another 10 rats from each group and in 10 normal rats. Results The survival time of donor hearts in the two EEPCW groups was significantly prolonged,to (15.9±2.4) days and (30.0±0.0) days,respectively,compared with (6.7±0.8) days in the control group. Pathological lesions in the two EEPCW groups were also less severe,and the percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and CD_4 +/CD_8 + ratio were significantly lower in the EEPCW groups. Conclusions Acute rejection of heart transplants and cellular immune reaction can be effectively suppressed using the EEPCW. Taking advantage of novel immunosuppressants derived from Chinese medicinal herbs used to treat abnormal pregnancy provides a hopeful road for future research and treatment in organ transplantation.展开更多
文摘为了探究茯苓配方(茯苓、山楂、黄大茶)浸膏对高脂饮食小鼠肥胖及脂质沉积的影响,将C57/BL6N小鼠随机分为对照组,模型组,奥利司他药物对照组,茯苓配方(Poria cocos Wolf formula,PWF)浸膏低、高剂量组。除对照组给予低脂饲料,其他组均以高脂饲料喂养12周。每周记录小鼠的体重、能量摄入。给药结束后,计算各组小鼠Lee’s指数、脏器系数,采用苏木精-伊红(hematoxylin-eosin,HE)染色法观察各组小鼠肝细胞与脂肪细胞形态变化,全自动生化仪测定各组小鼠血清中TG、TC、HDL-C、LDL-C、AST及ALT水平变化。结果显示,与对照组相比,模型组小鼠体重、Lee’s指数、脏器系数、TG、TC、LDL-C、AST及ALT水平均显著升高(P<0.01或P<0.001),肝脏脂滴数量与脂肪细胞直径增加;与模型组相比,茯苓配方浸膏高剂量组小鼠体重、Lee’s指数和脏器系数均显著降低(P<0.05或P<0.01),低剂量组小鼠体重、Lee’s指数和脏器系数均没有显著变化,茯苓配方浸膏高剂量组小鼠肝脏脂滴数量与脂肪细胞直径减少,TG、TC、LDL-C水平、AST及ALT水平均显著降低(P<0.05或P<0.01或P<0.001)。综上所述,茯苓配方浸膏能够改善高脂饮食小鼠的体重及脂质沉积,为开发改善肥胖的天然物质提供理论基础。
文摘Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effects on recurrent spontaneous abortion and that can maintain pregnancy until delivery. It was hypothesized that this herbal medicine can also prolong allograft survival after organ transplantation. Here,in an in vivo study,we report the anti-rejection effect of the ethanol extract of Poria cocos Wolf (EEPCW) in rats after cardiac allograft implantation. Methods Ten normal rats were healthy controls. Eighty rats receiving homologous heart transplants were divided into 4 groups of 20 rats each based on type of treatment: olive oil 8 ml·kg -1 ·d -1 ,EEPCW 25 mg·kg -1 ·d -1 , EEPCW 50 mg·kg -1 ·d -1 or cyclosporin A 5mg·kg -1 ·d -1 . Allograft survival was observed in 10 rats from each group. On the seventh day post transplantation,pathological lesions and percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and the CD_4 +/CD_8 + ratio in peripheral blood were assessed in another 10 rats from each group and in 10 normal rats. Results The survival time of donor hearts in the two EEPCW groups was significantly prolonged,to (15.9±2.4) days and (30.0±0.0) days,respectively,compared with (6.7±0.8) days in the control group. Pathological lesions in the two EEPCW groups were also less severe,and the percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and CD_4 +/CD_8 + ratio were significantly lower in the EEPCW groups. Conclusions Acute rejection of heart transplants and cellular immune reaction can be effectively suppressed using the EEPCW. Taking advantage of novel immunosuppressants derived from Chinese medicinal herbs used to treat abnormal pregnancy provides a hopeful road for future research and treatment in organ transplantation.
