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LysR family factor PltR positively regulates Pyoluteorin production in a pathway-specific manner in Pseudomonas sp. M18 被引量:5
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作者 YAN An, WANG XiaoLei, ZHANG XueHong & XU YuQuan Key Laboratory of Microbial Metabolism of Education Ministry, College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China 《Science China(Life Sciences)》 SCIE CAS 2007年第4期518-524,共7页
The pltR gene, coding a putative LysR-type regulator, was identified upstream Plt biosynthetic gene cluster in Pseudomonas sp. M18 using bioinformatics technology. The null mutation of pltR resulted in mutant M18TRG (... The pltR gene, coding a putative LysR-type regulator, was identified upstream Plt biosynthetic gene cluster in Pseudomonas sp. M18 using bioinformatics technology. The null mutation of pltR resulted in mutant M18TRG (pltR::Gm) by recombination and its Plt (Pyoluteorin) production declined to 30% while PCA (Phenazine-1-carboxylic acid) production remained unchanged as compared with the wild-type M18 grown in King’s Medium B. After complementation, Plt production of mutant M18TRG was restored to the level in wild-type M18. Overexpression of pltR in M18 led to 13-fold enhancement of Plt produc-tion over the wild-type M18 strain. However, PCA production was unchanged under this condition. These data suggested that PltR was a positive regulator on Plt production. Plt itself, however, could not regulate expression of pltR. Expression of the plt-lacZ transcriptional fusion in mutant M18TRG de-clined obviously as compared with the wild-type M18, which further proved that PltR could regulate expression of Plt biosynthetic genes at the transcriptional level. In addition, the investigation on the pltR expression in gacA mutant M18G and rsmA mutant M18R disclosed that PltR was involved in the positive regulation of gacA on Plt production while being excluded from the negative control caused by rsmA. 展开更多
关键词 LYSR family PSEUDOMONAS sp. M18 PYOLUTEORIN pltr TRANSCRIPTIONAL PROMOTION
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假单胞菌M18菌株的PltR因子特异性正调控藤黄绿菌素合成 被引量:1
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作者 严安 汪晓雷 +1 位作者 张雪洪 许煜泉 《中国科学(C辑)》 CSCD 北大核心 2007年第3期325-332,共8页
经生物信息学分析,在假单胞菌M18(Pseudomonas sp.M18)菌株的藤黄绿菌素(pyolute-orin,Plt)生物合成基因簇上游定位了一个属于LysR家族的调控因子编码基因pltR.运用同源重组技术,构建了pltR失活的突变菌株M18TRG,在King’sB培养基中,与... 经生物信息学分析,在假单胞菌M18(Pseudomonas sp.M18)菌株的藤黄绿菌素(pyolute-orin,Plt)生物合成基因簇上游定位了一个属于LysR家族的调控因子编码基因pltR.运用同源重组技术,构建了pltR失活的突变菌株M18TRG,在King’sB培养基中,与野生型菌株相比,Plt合成能力下降了70%,而吩嗪-1-羧酸(phenazine-1-carboxylic acid,PCA)的合成能力不受影响.反式互补pltR的突变株能回复Plt的合成能力达到野生型水平.在菌株M18中过表达pltR,Plt产量提高13倍,PCA产量没有改变.这些结果表明pltR基因表达产物是Plt生物合成的特异性正调控因子.在野生型菌株M18和不产Plt的突变株M18T中,pltR基因表达量无显著差异,表明pltR基因的表达不受Plt调控.与野生株相比,突变株M18TRG中的转录融合plt-lacZ表达量显著降低,表明PltR对Plt的正调控作用主要发生在转录水平上.对pltR基因在gacA突变株M18G和rsmA突变株M18R中的表达量的进一步研究发现,PltR参与了gacA基因对Plt的合成的正调控,但不参与rsmA基因对Plt合成的负调控. 展开更多
关键词 假单胞菌M18(Pseudomonas sp.M18)藤黄绿菌素 pltr 转录促进
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