Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam t...Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam throughsolvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessedin immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and theweights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSCon the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7days; on day 8, the plasma testosterone (T), estradiol (E_2) and LH were determined by RIA, the specific binding ofLH was estimated and the testes were weighed. (4) Effect of FSC on LH secretion was assessed in vitro on culturedadenohypophysis. (5) Effect of FSC on T secretion was assessed in vitro on Leydig cell culture. Results: FSC in-creased the weights of testis, epididymis and pituitary gland, and stimulated T and LH secretion both in vitro and inimmature rats. Conclusion: FSC invigorates the reproductive system and reproductive endocrine function in male rats.展开更多
Three human leucine-rich repeats and immunoglobulin-like domains （LRIG） genes and proteins, named LRIG1-3, has been previously characterized and it was proposed that they may act as suppressors of tumor growth. The ...Three human leucine-rich repeats and immunoglobulin-like domains （LRIG） genes and proteins, named LRIG1-3, has been previously characterized and it was proposed that they may act as suppressors of tumor growth. The LRIG1 protein can inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor. Real-time reverse transcription-polymerase chain reaction （RT-PCR） is a recently developed technique for quantitative assessment of specific RNA levels. In the current study, it was demonstrated that LRIG1-3 and EGFR mRNA was detected in human pituitary adenoma cell lines and a normal pituitary sample, with differences in the expression levels. Compared to the normal pituitary samples, the expression of LRIG1-3 in HP75 cell line was lower, but the expression of EGFR in HP75 cell line was higher. The results are consistent with LRIG1-3 being tumour suppressor genes, and LRIG genes decreasing the expression of EGFR. The ratio of EGFR/LRIGl was increased at least 13-fold in HP75 cells compared with the normal pituitary cells, which was also the case for the ratio of EGFR/LRIG2 （14-fold increase in HP75） and EGFR/LRIG3 （11-fold increase in HP75）. Further studies were needed to elucidate the explicit role of LRIG genes as negative regulators of oncogenesis in human pituitary adenoma.展开更多
文摘Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and en-docrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam throughsolvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessedin immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and theweights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSCon the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7days; on day 8, the plasma testosterone (T), estradiol (E_2) and LH were determined by RIA, the specific binding ofLH was estimated and the testes were weighed. (4) Effect of FSC on LH secretion was assessed in vitro on culturedadenohypophysis. (5) Effect of FSC on T secretion was assessed in vitro on Leydig cell culture. Results: FSC in-creased the weights of testis, epididymis and pituitary gland, and stimulated T and LH secretion both in vitro and inimmature rats. Conclusion: FSC invigorates the reproductive system and reproductive endocrine function in male rats.
文摘Three human leucine-rich repeats and immunoglobulin-like domains （LRIG） genes and proteins, named LRIG1-3, has been previously characterized and it was proposed that they may act as suppressors of tumor growth. The LRIG1 protein can inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor. Real-time reverse transcription-polymerase chain reaction （RT-PCR） is a recently developed technique for quantitative assessment of specific RNA levels. In the current study, it was demonstrated that LRIG1-3 and EGFR mRNA was detected in human pituitary adenoma cell lines and a normal pituitary sample, with differences in the expression levels. Compared to the normal pituitary samples, the expression of LRIG1-3 in HP75 cell line was lower, but the expression of EGFR in HP75 cell line was higher. The results are consistent with LRIG1-3 being tumour suppressor genes, and LRIG genes decreasing the expression of EGFR. The ratio of EGFR/LRIGl was increased at least 13-fold in HP75 cells compared with the normal pituitary cells, which was also the case for the ratio of EGFR/LRIG2 （14-fold increase in HP75） and EGFR/LRIG3 （11-fold increase in HP75）. Further studies were needed to elucidate the explicit role of LRIG genes as negative regulators of oncogenesis in human pituitary adenoma.
