The inhibition of Piperol B on rabbit platelet aggregation induced by PAF and its mechanism were studied.Piperol B and ginkgolid B (BN52021) dose dependently inhibited rabbit platelet aggregation induced by PAF, IC50s...The inhibition of Piperol B on rabbit platelet aggregation induced by PAF and its mechanism were studied.Piperol B and ginkgolid B (BN52021) dose dependently inhibited rabbit platelet aggregation induced by PAF, IC50s were 14. 2, 4. 78μmol· L-1. But Piperol B (100, 200μmol· L-1) had no significant effect on aggregation of rabbit platelet induced by AA and ADP. Either increase of cytoplasmic free calcium[Ca2+]i or calcium influx induced by PAF was dose dependently inhibited by Piperol B (10, 100μmol· L-1). The maximum inhibition was 50. 8% and 37. 2%,respectively. But Piperol B had no effect on the increase of [Ca2+]i induced by A23187. Our results suggest that Piperol B selectively inhibits aggregation of rabbit platelet induced by PAF, and this effect is concerned with its inhibition on increase of [Ca2+]i and Ca2+ influx induced by PAF.展开更多
文摘The inhibition of Piperol B on rabbit platelet aggregation induced by PAF and its mechanism were studied.Piperol B and ginkgolid B (BN52021) dose dependently inhibited rabbit platelet aggregation induced by PAF, IC50s were 14. 2, 4. 78μmol· L-1. But Piperol B (100, 200μmol· L-1) had no significant effect on aggregation of rabbit platelet induced by AA and ADP. Either increase of cytoplasmic free calcium[Ca2+]i or calcium influx induced by PAF was dose dependently inhibited by Piperol B (10, 100μmol· L-1). The maximum inhibition was 50. 8% and 37. 2%,respectively. But Piperol B had no effect on the increase of [Ca2+]i induced by A23187. Our results suggest that Piperol B selectively inhibits aggregation of rabbit platelet induced by PAF, and this effect is concerned with its inhibition on increase of [Ca2+]i and Ca2+ influx induced by PAF.
