测定了2羟-基-4-甲氧基苯甲醛和2羟-基-4-甲氧基苯甲酸两种效应物对菜粉蝶多酚氧化酶(po lypheno lox idase,简称PPO,EC.1.14.18.1)催化L-多巴(L-DOPA)氧化活力的抑制作用。结果表明,这两种效应物对该酶的活性均有明显的抑制作用,抑制...测定了2羟-基-4-甲氧基苯甲醛和2羟-基-4-甲氧基苯甲酸两种效应物对菜粉蝶多酚氧化酶(po lypheno lox idase,简称PPO,EC.1.14.18.1)催化L-多巴(L-DOPA)氧化活力的抑制作用。结果表明,这两种效应物对该酶的活性均有明显的抑制作用,抑制中浓度(IC50)分别为2.71和5.66 mm o l/L,抑制常数分别为2.82和3.35 mm o l/L;2羟-基-4-甲氧基苯甲醛对该酶的抑制作用显著高于2羟-基-4-甲氧基苯甲酸的;这两种抑制剂对酶的抑制作用机理完全不同,2羟-基-4-甲氧基苯甲醛对酶的作用表现为混合型抑制,而2羟-基-4-甲氧基苯甲酸对酶的作用表现为竞争性抑制。研究结果为设计以该酶为靶标的杀虫剂提供理论依据。展开更多
The non alkaloid contents were extracted from the root bark of Tripterygium wilfordii Hook. The results of bioassy showed that the non alkaloid extracts has abviously insectidal activities to imported cabbage worm ( P...The non alkaloid contents were extracted from the root bark of Tripterygium wilfordii Hook. The results of bioassy showed that the non alkaloid extracts has abviously insectidal activities to imported cabbage worm ( Pieris rapae L.).LC 50 values of poisoning to the 5th instarts of Pieris rapae is 7.23 mg·L -1 . The values of medium antifeeding concentration (AFC 50 ) of the non alkaloid extracted against the 5th larval of Pieris rape is 11.34 mg·L -1 . Median narcosis dose (ND 50 ) is 15.54 μg/g.Median contact dose (LD 50 ) is 3.1079 μg/head. The non alkaloid extracts have no fumigant to insect. The main poisoning symptoms are that the tested insect showed paralysis and the epiderm of the larval turns black, the larval are dead at last.展开更多
文摘在温度为20～22℃的实验室条件下,用AfMNPV和PrGV的混合病毒悬液感染3龄期菜粉蝶幼虫,可有效降低2种病毒的用量,并显示出良好的增效作用,PrGV和AfMNPV毒力倍数分别为单剂的600倍和400倍以上.不同浓度混合病毒悬液的LT50分别比AfMNPV和PrGV单剂的LT50缩短1.10～2.06 d和0.40～1.93 d.用PrGV病毒悬液感染4龄期菜粉蝶幼虫,其LT50比3龄期增加3.43～6.06 d.
文摘测定了2羟-基-4-甲氧基苯甲醛和2羟-基-4-甲氧基苯甲酸两种效应物对菜粉蝶多酚氧化酶(po lypheno lox idase,简称PPO,EC.1.14.18.1)催化L-多巴(L-DOPA)氧化活力的抑制作用。结果表明,这两种效应物对该酶的活性均有明显的抑制作用,抑制中浓度(IC50)分别为2.71和5.66 mm o l/L,抑制常数分别为2.82和3.35 mm o l/L;2羟-基-4-甲氧基苯甲醛对该酶的抑制作用显著高于2羟-基-4-甲氧基苯甲酸的;这两种抑制剂对酶的抑制作用机理完全不同,2羟-基-4-甲氧基苯甲醛对酶的作用表现为混合型抑制,而2羟-基-4-甲氧基苯甲酸对酶的作用表现为竞争性抑制。研究结果为设计以该酶为靶标的杀虫剂提供理论依据。
文摘The non alkaloid contents were extracted from the root bark of Tripterygium wilfordii Hook. The results of bioassy showed that the non alkaloid extracts has abviously insectidal activities to imported cabbage worm ( Pieris rapae L.).LC 50 values of poisoning to the 5th instarts of Pieris rapae is 7.23 mg·L -1 . The values of medium antifeeding concentration (AFC 50 ) of the non alkaloid extracted against the 5th larval of Pieris rape is 11.34 mg·L -1 . Median narcosis dose (ND 50 ) is 15.54 μg/g.Median contact dose (LD 50 ) is 3.1079 μg/head. The non alkaloid extracts have no fumigant to insect. The main poisoning symptoms are that the tested insect showed paralysis and the epiderm of the larval turns black, the larval are dead at last.
