The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resi...The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants’ genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K+ and Na+ showed that transgenic lines were able to retain less Na+ than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.展开更多
In the present article, we argue that consciousness and body are not to be separate to explain the intentional consciousness of the human being facing to environment. 1) The most neurobiologist and neurocognitivist st...In the present article, we argue that consciousness and body are not to be separate to explain the intentional consciousness of the human being facing to environment. 1) The most neurobiologist and neurocognitivist studies suggest that the emerging consciousness appears to be a generic recurring process which subsumes under it the various results of scientist approaches. When analyzed with the cybernetic transductive method of Simondon, this process can be viewed as a noetico-neuronal unit which the sui generis activity can be boiled down to concept of intentional consciousness. 2) Homeostasis can be also metaphorically described as a generic recurring process which subsumes under it the myriad molecular feedback loops of organism. This self-regulating dynamic process could be governed by the cosmologic (Earth + Universe) production of entropy, which would keep high activity and dissipating energy and low internal entropy to maintain an ordering in the organic structure. 3) Based on numerous arguments and data, the cerebral noetico-neuronal process and the self-regulating homeostasic process may be viewed as an entire physiological (brain + body) system. We suggest that the whole (brain + body) has an intentional embodied consciousness that could be driven by the cosmologic production of entropy.展开更多
KiSS-1,first identified as an anti-metastasis gene in melanoma,encodes C-terminally amidated peptide products,including kisspeptin-145,kisspeptin-54,kisspeptin-14,kisspeptin-13 and kisspeptin-10.These products are end...KiSS-1,first identified as an anti-metastasis gene in melanoma,encodes C-terminally amidated peptide products,including kisspeptin-145,kisspeptin-54,kisspeptin-14,kisspeptin-13 and kisspeptin-10.These products are endogenous ligands coupled to G protein-coupled receptor 54(GPR54)/hOT7T175/AXOR12.To date,the regulatory activities of the KiSS-1/GPR54 system,such as puberty initiation,antitumor metastasis,fertility in adulthood,hypothalamic-pituitary-gonadal axis(HPG axis)feedback,and trophoblast invasion,have been investigated intensively.Accumulating evidence has demonstrated that KiSS-1 played a key role in reproduction and served as a promising biomarker relative to the diagnosis,identification of therapeutic targets and prognosis in various carcinomas,while few studies have systematically summarized its subjective factors and concluded the functions of KiSS-1/GPR54 signaling in physiology homeostasis and cancer biology.In this review,we retrospectively summarized the regulators of the KiSS-1/GPR54 system in different animal models and reviewed its functions according to physiological homeostasis regulations and above all,cancer biology,which provided us with a profound understanding of applying the KiSS-1/GPR54 system into medical applications.展开更多
基金a grant from State 863 National High Technology Research Development Project of China, No. 819-0803.
文摘The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants’ genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K+ and Na+ showed that transgenic lines were able to retain less Na+ than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.
文摘In the present article, we argue that consciousness and body are not to be separate to explain the intentional consciousness of the human being facing to environment. 1) The most neurobiologist and neurocognitivist studies suggest that the emerging consciousness appears to be a generic recurring process which subsumes under it the various results of scientist approaches. When analyzed with the cybernetic transductive method of Simondon, this process can be viewed as a noetico-neuronal unit which the sui generis activity can be boiled down to concept of intentional consciousness. 2) Homeostasis can be also metaphorically described as a generic recurring process which subsumes under it the myriad molecular feedback loops of organism. This self-regulating dynamic process could be governed by the cosmologic (Earth + Universe) production of entropy, which would keep high activity and dissipating energy and low internal entropy to maintain an ordering in the organic structure. 3) Based on numerous arguments and data, the cerebral noetico-neuronal process and the self-regulating homeostasic process may be viewed as an entire physiological (brain + body) system. We suggest that the whole (brain + body) has an intentional embodied consciousness that could be driven by the cosmologic production of entropy.
基金This work was supported by National Natural Science Foundation of China(No.81902754 to LD,81772880 to YN and 81702680 to YS)China Postdoctoral Science Foundation(No.2019M651789 to LD)+2 种基金Fundamental Research Funds for the Central Universities(No.021014380161 to LD)Natural Science Foundation of Jiangsu Province(No.BK20190304 to LD)Nanjing Medical Science and Technology Development Foundation,Nanjing Department of Health(No.YKK17138 to YS).
文摘KiSS-1,first identified as an anti-metastasis gene in melanoma,encodes C-terminally amidated peptide products,including kisspeptin-145,kisspeptin-54,kisspeptin-14,kisspeptin-13 and kisspeptin-10.These products are endogenous ligands coupled to G protein-coupled receptor 54(GPR54)/hOT7T175/AXOR12.To date,the regulatory activities of the KiSS-1/GPR54 system,such as puberty initiation,antitumor metastasis,fertility in adulthood,hypothalamic-pituitary-gonadal axis(HPG axis)feedback,and trophoblast invasion,have been investigated intensively.Accumulating evidence has demonstrated that KiSS-1 played a key role in reproduction and served as a promising biomarker relative to the diagnosis,identification of therapeutic targets and prognosis in various carcinomas,while few studies have systematically summarized its subjective factors and concluded the functions of KiSS-1/GPR54 signaling in physiology homeostasis and cancer biology.In this review,we retrospectively summarized the regulators of the KiSS-1/GPR54 system in different animal models and reviewed its functions according to physiological homeostasis regulations and above all,cancer biology,which provided us with a profound understanding of applying the KiSS-1/GPR54 system into medical applications.
文摘从功能内稳态(function-specific homeostasis,FSH)角度分析EIMD/DOMS过程影响运动成绩的正常发挥,而防治措施又大多无效这一现象,结果发现力竭运动所致的EIMD/DOMS,骨骼肌蛋白质代谢远离了蛋白质代谢内稳态(protein metabolite specific homeostasis,PmSH),EIMD/DOMS康复延迟,外源性有利因素干预可促进EIMD/DOMS康复;非力竭运动所致的EIMD/DOMS,蛋白质代谢处于PmSH,EIMD/DOMS正常康复,外源性因素对EIMD/DOMS无明显影响作用。
