卵黄高磷蛋白是已知天然蛋白中含磷量最高的蛋白,经适宜蛋白酶水解后形成磷酸肽,该磷酸肽能够与Ca2+、Fe2+、Mg2+等二价金属离子结合形成可溶性络合物,抑制磷酸钙等非可溶性磷酸盐的形成,从而促进机体对钙、铁的吸收。本文主要探索了不...卵黄高磷蛋白是已知天然蛋白中含磷量最高的蛋白,经适宜蛋白酶水解后形成磷酸肽,该磷酸肽能够与Ca2+、Fe2+、Mg2+等二价金属离子结合形成可溶性络合物,抑制磷酸钙等非可溶性磷酸盐的形成,从而促进机体对钙、铁的吸收。本文主要探索了不同缓冲溶液、不同稀释程度、不同p H值等因素对卵黄高磷蛋白提取效果的影响,并在单因素实验的基础上采用正交试验,进一步优化了卵黄高磷蛋白的提取工艺。结果表明,应用10倍体积的0.05 M的p H 5.2的醋酸-醋酸钠溶液提取的卵黄高磷蛋白效果较好,每10 m L的卵黄可以提取约90mg的卵黄高磷蛋白。提取的卵黄高磷蛋白成品含磷量为7.2%,N/P比为3.9。经过进一步脱磷、酶解等处理制备了卵黄高磷蛋白磷酸肽,该磷酸肽能明显阻滞磷酸钙沉淀的形成,每100 mg能够结合9.1 mg的钙。本实验结果,为后续动物体内补钙功能实验及相关磷酸肽补钙制剂的研发奠定了基础。展开更多
Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM...Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM)mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone formation.The serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment(2.19 mmol/L),and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase(AKP)caused by calcium deficiency.The low-calcium control group had the lowest calcium deposited to the femur(80.41 mg/g)and the lowest femur bone mineral density(BMD)(0.17 g/cm^(3)),while HTMP-Enz-PPP-Ca significantly improved bone calcium content(94.33 mg/g)and BMD(0.29 g/cm^(3)).The micro-computed tomography(MCT)images showed that the femur with the normal control,PV-Ca,and HTMP-Enz-PPP-Ca treatments had a more compact,complete,and thicker trabecular network than the low-calcium and CaCl_(2)treatments.These results indicated that the organic calcium(HTMP-Enz-PPP-Ca)promoted calcium absorption and bone deposition,and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl_(2).展开更多
文摘卵黄高磷蛋白是已知天然蛋白中含磷量最高的蛋白,经适宜蛋白酶水解后形成磷酸肽,该磷酸肽能够与Ca2+、Fe2+、Mg2+等二价金属离子结合形成可溶性络合物,抑制磷酸钙等非可溶性磷酸盐的形成,从而促进机体对钙、铁的吸收。本文主要探索了不同缓冲溶液、不同稀释程度、不同p H值等因素对卵黄高磷蛋白提取效果的影响,并在单因素实验的基础上采用正交试验,进一步优化了卵黄高磷蛋白的提取工艺。结果表明,应用10倍体积的0.05 M的p H 5.2的醋酸-醋酸钠溶液提取的卵黄高磷蛋白效果较好,每10 m L的卵黄可以提取约90mg的卵黄高磷蛋白。提取的卵黄高磷蛋白成品含磷量为7.2%,N/P比为3.9。经过进一步脱磷、酶解等处理制备了卵黄高磷蛋白磷酸肽,该磷酸肽能明显阻滞磷酸钙沉淀的形成,每100 mg能够结合9.1 mg的钙。本实验结果,为后续动物体内补钙功能实验及相关磷酸肽补钙制剂的研发奠定了基础。
基金supported by the General Program of National Natural Science Foundation of China(32072237)the Hubei Provincial Natural Science Foundation of China(2020CFB583)Fundamental Research Funds for the Central Universities(2662020SPPY006)。
文摘Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were prepared.The low-calcium specific pathogen free-Kunming(SPF-KM)mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone formation.The serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment(2.19 mmol/L),and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase(AKP)caused by calcium deficiency.The low-calcium control group had the lowest calcium deposited to the femur(80.41 mg/g)and the lowest femur bone mineral density(BMD)(0.17 g/cm^(3)),while HTMP-Enz-PPP-Ca significantly improved bone calcium content(94.33 mg/g)and BMD(0.29 g/cm^(3)).The micro-computed tomography(MCT)images showed that the femur with the normal control,PV-Ca,and HTMP-Enz-PPP-Ca treatments had a more compact,complete,and thicker trabecular network than the low-calcium and CaCl_(2)treatments.These results indicated that the organic calcium(HTMP-Enz-PPP-Ca)promoted calcium absorption and bone deposition,and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl_(2).
