目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增...目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增殖和腹腔巨噬细胞吞噬活性的影响。结果甘露糖可以强烈的抑制细菌内化,灭活和四甲基脲处理可以较明显的减低细菌内化。活菌和灭活菌可以特异性的调节腹腔巨噬细胞、PP细胞和脾淋巴细胞的活性。结论细菌表面甘露糖糖凝集素是影响细菌PP内化的最主要因素。细菌活力、表面疏水性也有一定的作用。活菌和灭活可以不同程度的增强小鼠腹腔巨噬细胞的吞噬活性并抑制脾和PP淋巴细胞增殖。展开更多
Microfold (M) cells are a kind of intestinal epithelial cell in the follicle-associated epithelium (FAE) of Peyer’s patches. They can transport antigens and microorganisms to lymphoid tissues. Bovine spongiform encep...Microfold (M) cells are a kind of intestinal epithelial cell in the follicle-associated epithelium (FAE) of Peyer’s patches. They can transport antigens and microorganisms to lymphoid tissues. Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder in cattle. It is linked to variant Creutzfeldt-Jakob disease in humans. Although it is thought that M cells transport the BSE agent, the exact mechanism by which it crosses the intestinal barrier is not clear. We have bovine intestinal epithelial cell line (BIE cells), which can differentiate into the M cell type in vitro after stimulation, and which is able to transport the BSE agent. We show here that M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles, compared with non-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Aldolase A was co-stained with incorporated PrPSc in M-BIE cells. These results suggest that bovine M cells and goblet cells synthesize aldolase A, and that aldolase A may have the ability to bind PrP and associate with PrP in cellular vesicles. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.展开更多
文摘目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增殖和腹腔巨噬细胞吞噬活性的影响。结果甘露糖可以强烈的抑制细菌内化,灭活和四甲基脲处理可以较明显的减低细菌内化。活菌和灭活菌可以特异性的调节腹腔巨噬细胞、PP细胞和脾淋巴细胞的活性。结论细菌表面甘露糖糖凝集素是影响细菌PP内化的最主要因素。细菌活力、表面疏水性也有一定的作用。活菌和灭活可以不同程度的增强小鼠腹腔巨噬细胞的吞噬活性并抑制脾和PP淋巴细胞增殖。
文摘Microfold (M) cells are a kind of intestinal epithelial cell in the follicle-associated epithelium (FAE) of Peyer’s patches. They can transport antigens and microorganisms to lymphoid tissues. Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder in cattle. It is linked to variant Creutzfeldt-Jakob disease in humans. Although it is thought that M cells transport the BSE agent, the exact mechanism by which it crosses the intestinal barrier is not clear. We have bovine intestinal epithelial cell line (BIE cells), which can differentiate into the M cell type in vitro after stimulation, and which is able to transport the BSE agent. We show here that M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles, compared with non-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Aldolase A was co-stained with incorporated PrPSc in M-BIE cells. These results suggest that bovine M cells and goblet cells synthesize aldolase A, and that aldolase A may have the ability to bind PrP and associate with PrP in cellular vesicles. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.
