Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Me...Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Methods We compared the peritoneal macrophage activity obtained from the irrigation solution of theuremia patients (n=2) immediately after inserting the catheter and with those from the long - dwell time dialysate(≥10h) of peritoneal dialysis patients (n= 7). The capacity of peritoneal macrophage to release TNF - x was assayedby L929 cytotoxicity. At the same time, we compared TNF- 7 activity among different glucose concentration (1.5%,2.5% and 4.25% dextrose) of dialysate. Results The levels of TNF- a in CAPD and control group were 170 and517 pg/106 respectively (P<0.01). The patients who were using dialysate containing higher glucose concentrationhad lower TNF- x level (P<0.05, P<0.01). Conclusion It is suggested that the inhibitory effect of peritonealdialysate on peritoneal macrophages might continue at least 10h in vivo. The higher glucose concentration ofdialysate may have worse effect on peritoneal macrophage activity.展开更多
目的:建立腹膜透析液中头孢呋辛钠的含量测定方法并考察二者配伍稳定性。方法:采用高效液相色谱法测定头孢呋辛钠含量,色谱柱为Phenomenex Luna C18,流动相为乙腈-0.05mol·L-1醋酸钠缓冲液(pH3.9)=10∶90,流速为1.0mL·min-1,...目的:建立腹膜透析液中头孢呋辛钠的含量测定方法并考察二者配伍稳定性。方法:采用高效液相色谱法测定头孢呋辛钠含量,色谱柱为Phenomenex Luna C18,流动相为乙腈-0.05mol·L-1醋酸钠缓冲液(pH3.9)=10∶90,流速为1.0mL·min-1,检测波长为273nm;考察头孢呋辛钠水溶液、头孢呋辛钠腹膜透析液放置6h后的不溶性微粒、澄清度、外观性状、pH值及含量。结果:头孢呋辛钠检测浓度线性范围为1.25～125μg·mL-1(r=0.9998),平均回收率为99.6%,RSD为0.6%(n=6);2种溶液6h内各检查指标均无明显变化。结论:所建立的含量测定方法简单、准确、灵敏,可用于腹膜透析液中头孢呋辛钠的含量测定;头孢呋辛钠与腹膜透析液配伍6h内稳定性良好。展开更多
The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor-β1 （TGF-β1） plays an important role in regulating tissue repair and remodeling after injury. Excessive synthesis ...The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor-β1 （TGF-β1） plays an important role in regulating tissue repair and remodeling after injury. Excessive synthesis and deposition of matrix proteins by peritoneal mesothelial cells can lead to structural and functional changes in the peritoneal membrane, jeopardizing the long-term efficacy of peritoneal dialysis （PD）. Prolonged exposure to high glucose concentrations in PD fluid has been implicated as a major stimulus to matrix accumulation, through the induction of transforming growth factor-β1 （TGF-β1）.展开更多
文摘Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Methods We compared the peritoneal macrophage activity obtained from the irrigation solution of theuremia patients (n=2) immediately after inserting the catheter and with those from the long - dwell time dialysate(≥10h) of peritoneal dialysis patients (n= 7). The capacity of peritoneal macrophage to release TNF - x was assayedby L929 cytotoxicity. At the same time, we compared TNF- 7 activity among different glucose concentration (1.5%,2.5% and 4.25% dextrose) of dialysate. Results The levels of TNF- a in CAPD and control group were 170 and517 pg/106 respectively (P<0.01). The patients who were using dialysate containing higher glucose concentrationhad lower TNF- x level (P<0.05, P<0.01). Conclusion It is suggested that the inhibitory effect of peritonealdialysate on peritoneal macrophages might continue at least 10h in vivo. The higher glucose concentration ofdialysate may have worse effect on peritoneal macrophage activity.
文摘目的:建立腹膜透析液中头孢呋辛钠的含量测定方法并考察二者配伍稳定性。方法:采用高效液相色谱法测定头孢呋辛钠含量,色谱柱为Phenomenex Luna C18,流动相为乙腈-0.05mol·L-1醋酸钠缓冲液(pH3.9)=10∶90,流速为1.0mL·min-1,检测波长为273nm;考察头孢呋辛钠水溶液、头孢呋辛钠腹膜透析液放置6h后的不溶性微粒、澄清度、外观性状、pH值及含量。结果:头孢呋辛钠检测浓度线性范围为1.25～125μg·mL-1(r=0.9998),平均回收率为99.6%,RSD为0.6%(n=6);2种溶液6h内各检查指标均无明显变化。结论:所建立的含量测定方法简单、准确、灵敏,可用于腹膜透析液中头孢呋辛钠的含量测定;头孢呋辛钠与腹膜透析液配伍6h内稳定性良好。
文摘The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor-β1 （TGF-β1） plays an important role in regulating tissue repair and remodeling after injury. Excessive synthesis and deposition of matrix proteins by peritoneal mesothelial cells can lead to structural and functional changes in the peritoneal membrane, jeopardizing the long-term efficacy of peritoneal dialysis （PD）. Prolonged exposure to high glucose concentrations in PD fluid has been implicated as a major stimulus to matrix accumulation, through the induction of transforming growth factor-β1 （TGF-β1）.
