Aim: Phosphatase and tension homolog (PTEN) has been known to maintain homeostatic control over the body. The roles of PTEN in periodontal complex are unknown. The purpose of this study was to investigate the role of ...Aim: Phosphatase and tension homolog (PTEN) has been known to maintain homeostatic control over the body. The roles of PTEN in periodontal complex are unknown. The purpose of this study was to investigate the role of PTEN in periodontal structures by removing PTEN from osteoblasts and odontoblasts. Materials and Methods: The function of this endogenous PTEN was evaluated by conditionally eliminating the PTEN gene using an Osteocalcin (OCN) Cre driver. The resulting OCN-Cre<sup>tg/+</sup>;Pten<sup>fl/fl </sup>mice were examined using micro-CT and histology, immunohistochemical analyses for osteogenic markers in the periodontal ligament (PDL) and bone turnover. Results: Bone apposition was increased around molar areas accompanying deposition of cementum in micro CT. Osteoprogenitor markers except for OCN in the PDL maintained their expression in both wild-type and OCN-Cre<sup>tg/+</sup>;Pten<sup>fl/fl</sup> mice. Both alkaline phosphatase activity and osteoclast activity increased in the PDL of OCN-Cre<sup>tg/+</sup>;Pten<sup>fl/fl</sup> mice compared to those in wild-type mice. Conclusions: Loss of PTEN causes an increase of bone turnover in the periodontal surrounding tissues with an increase of cementogenesis. These findings underscore the effect of PTEN on homeostasis of the periodontal ligament.展开更多
研究牛牙周膜宽度和胶原纤维容量比与弹性模量的相关性及其定量关系。通过实验测定新鲜成牛牙周膜标准试件中牙周膜的长度、宽度以及弹性模量。用1%苦味酸天狼星红染色剂使试件断面胶原纤维染色,体视显微镜下观察采图,借助Image pro plu...研究牛牙周膜宽度和胶原纤维容量比与弹性模量的相关性及其定量关系。通过实验测定新鲜成牛牙周膜标准试件中牙周膜的长度、宽度以及弹性模量。用1%苦味酸天狼星红染色剂使试件断面胶原纤维染色,体视显微镜下观察采图,借助Image pro plus6.0图像分析软件测量并计算纤维容量比。结果表明,成牛牙周膜宽度与弹性模量呈负相关,相关系数r=-0.021,简单线性回归模型为y=71.681-0.021x(宽度);纤维容量比与牙周膜弹性模量呈正相关,相关系数r=1.583,简单线性回归模型为y=-34.944+1.583x(百分比)。所以,成牛牙周膜的弹性模量随着牙周膜的宽度增加而减小,随着纤维容量比的增加而增大。展开更多
文摘Aim: Phosphatase and tension homolog (PTEN) has been known to maintain homeostatic control over the body. The roles of PTEN in periodontal complex are unknown. The purpose of this study was to investigate the role of PTEN in periodontal structures by removing PTEN from osteoblasts and odontoblasts. Materials and Methods: The function of this endogenous PTEN was evaluated by conditionally eliminating the PTEN gene using an Osteocalcin (OCN) Cre driver. The resulting OCN-Cre<sup>tg/+</sup>;Pten<sup>fl/fl </sup>mice were examined using micro-CT and histology, immunohistochemical analyses for osteogenic markers in the periodontal ligament (PDL) and bone turnover. Results: Bone apposition was increased around molar areas accompanying deposition of cementum in micro CT. Osteoprogenitor markers except for OCN in the PDL maintained their expression in both wild-type and OCN-Cre<sup>tg/+</sup>;Pten<sup>fl/fl</sup> mice. Both alkaline phosphatase activity and osteoclast activity increased in the PDL of OCN-Cre<sup>tg/+</sup>;Pten<sup>fl/fl</sup> mice compared to those in wild-type mice. Conclusions: Loss of PTEN causes an increase of bone turnover in the periodontal surrounding tissues with an increase of cementogenesis. These findings underscore the effect of PTEN on homeostasis of the periodontal ligament.
文摘研究牛牙周膜宽度和胶原纤维容量比与弹性模量的相关性及其定量关系。通过实验测定新鲜成牛牙周膜标准试件中牙周膜的长度、宽度以及弹性模量。用1%苦味酸天狼星红染色剂使试件断面胶原纤维染色,体视显微镜下观察采图,借助Image pro plus6.0图像分析软件测量并计算纤维容量比。结果表明,成牛牙周膜宽度与弹性模量呈负相关,相关系数r=-0.021,简单线性回归模型为y=71.681-0.021x(宽度);纤维容量比与牙周膜弹性模量呈正相关,相关系数r=1.583,简单线性回归模型为y=-34.944+1.583x(百分比)。所以,成牛牙周膜的弹性模量随着牙周膜的宽度增加而减小,随着纤维容量比的增加而增大。
