Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques ...Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques （COSY, HMQC, HMBC, NOESY） and HR-ESIMS.展开更多
以三月红荔枝果实膨大期果皮为试材,通过采用改良CTAB法、SDS法、改良SDS法、改良Bugos法和RNAplant plus Reagent试剂盒(TIANGEN公司)提取总RNA,采用浓度检测、纯度检测、RT-PCR等方法进行提取结果检测,进而筛选出最佳提取方法。结果表...以三月红荔枝果实膨大期果皮为试材,通过采用改良CTAB法、SDS法、改良SDS法、改良Bugos法和RNAplant plus Reagent试剂盒(TIANGEN公司)提取总RNA,采用浓度检测、纯度检测、RT-PCR等方法进行提取结果检测,进而筛选出最佳提取方法。结果表明,这5种方法都能从三月红荔枝果皮中提取总RNA,但总RNA浓度和纯度有明显的差异。其中采用改良Bugos法、改良SDS法和RNAplant plus Reagen试剂盒提取RNA的浓度较高;采用改良CTAB法和RNAplant plus Reagen试剂盒提取的总RNA纯度较高,完整性较好。采用SDS法、改良SDS法和改良Bugos法所提取的荔枝果皮总RNA纯度低,降解严重。经RT-PCR验证,采用改良CTAB法和RNAplant plus Reagen试剂盒均能扩增得到200bp的18sRNA编码基因片段。发现改良CTAB法为其中提取荔枝果皮总RNA的最佳方法,总RNA质量和得率满足后续分子生物学实验的需要。展开更多
文摘Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques （COSY, HMQC, HMBC, NOESY） and HR-ESIMS.
文摘以三月红荔枝果实膨大期果皮为试材,通过采用改良CTAB法、SDS法、改良SDS法、改良Bugos法和RNAplant plus Reagent试剂盒(TIANGEN公司)提取总RNA,采用浓度检测、纯度检测、RT-PCR等方法进行提取结果检测,进而筛选出最佳提取方法。结果表明,这5种方法都能从三月红荔枝果皮中提取总RNA,但总RNA浓度和纯度有明显的差异。其中采用改良Bugos法、改良SDS法和RNAplant plus Reagen试剂盒提取RNA的浓度较高;采用改良CTAB法和RNAplant plus Reagen试剂盒提取的总RNA纯度较高,完整性较好。采用SDS法、改良SDS法和改良Bugos法所提取的荔枝果皮总RNA纯度低,降解严重。经RT-PCR验证,采用改良CTAB法和RNAplant plus Reagen试剂盒均能扩增得到200bp的18sRNA编码基因片段。发现改良CTAB法为其中提取荔枝果皮总RNA的最佳方法,总RNA质量和得率满足后续分子生物学实验的需要。
