目的探讨影响AIDS合并马尔尼菲青霉菌病（PSM）预后的因素。方法165例经病嗯学确诊的AIDS合并PSM患者，根据预岳分成牛存组和死亡组。应用Logistic回归，分析2j个可能影响疾病预后的临床和实验室指标．筛选出独立预后因素。结果AIDS合并...目的探讨影响AIDS合并马尔尼菲青霉菌病（PSM）预后的因素。方法165例经病嗯学确诊的AIDS合并PSM患者，根据预岳分成牛存组和死亡组。应用Logistic回归，分析2j个可能影响疾病预后的临床和实验室指标．筛选出独立预后因素。结果AIDS合并PSM患者中。生有组121例，死亡组44例，病死率为26．7％。单因素分析显示．影响AIDS合并PSM预后的因素包括皮疹、细菌和（或）其他真菌性肺炎、CMV感染、败血症、肾功能损伤、PLT减少、Hb、CD4-T淋巴细胞计数、Alb、伊曲康唑（ITR）广子贯疗法及高效抗反转录病毒治疗（HAART）。多因素分析显示，去除各因素之间相互混杂的影响，筛选出细菌或其他真菌性甘市炎（OR=6．539；95％CI2．312，18．498）、败血症（OR=15．307；95%,CI1．473．42．462）及PLT减少（OR=7．763：95％CI2．658，22．675）是AIDS合并PSM死亡的危险因素，而ITR序贯疗法（OR=0．088；95％CI 0．013．0．611）与HAART（OR=0．084；95% CI 0．026，0．269）是其保护性因素。结论合并细菌或其他真菌性肺炎、败血症与PLT减少是AIDS合并PSM预后不良的危险因素，ITR序贯疗法和HAART则可减少死亡风险，改善预后；展开更多
Filamentous fungi are able to accumulate significant amount of metals from their environment. The potential of fungal biomass as agents for biosorption of heavy metals from contaminated sediments is currently receivin...Filamentous fungi are able to accumulate significant amount of metals from their environment. The potential of fungal biomass as agents for biosorption of heavy metals from contaminated sediments is currently receiving attention. In the present study, a total of 41 isolates of filamentous fungi obtained from the sediment of the Langat River, Selangor, Malaysia were screened for their tolerance and uptake capability of copper （Cu） and lead （Pb）. The isolates were identified as Aspergillus niger, A. fumigatus, Trichoderma asperellum, Penicillium simplicissimurn and P. janthinellum. A. niger and P. simplicissimum, were able to survive at 1000 mg/L of Cu（Ⅱ） concentration on Potato Dextrose Agar （PDA） while for Pb, only A. niger survived at 5000 mg/L concentration. The results showed that A. niger, P. simplicissimum and T. asperellum have a better uptake capacity for Pb compared to Cu and the findings indicated promising biosorption of Cu and Pb by these filamentous fungi from aqueous solution. The present study was also determined the maximum removal of Cu（Ⅱ） and Pb（Ⅱ） that was performed by A. niger. The metal removal which occurred at Cu（Ⅱ） 200 mg/L was （20.910 ＋ 0.581） mg/g and at 250 mg/L of PbⅡ） was （54.046 ± 0.328） mg/g.展开更多
Three antagonistic yeasts, Trichosporon pullulans (Lindner.) Diddens and Lodder, Cryptococcus laurentii (Kuffer.) Skinner and Rhodotorula glutinis (Fresen.) F. C. Harrison, selected from over 50 yeasts on apple fruits...Three antagonistic yeasts, Trichosporon pullulans (Lindner.) Diddens and Lodder, Cryptococcus laurentii (Kuffer.) Skinner and Rhodotorula glutinis (Fresen.) F. C. Harrison, selected from over 50 yeasts on apple fruits, were investigated of their biocontrol efficacy against blue mould rot caused by Penicillium expansum Link in apple fruits. T. pullulans was identified to be a new yeast antagonist and was the most effective at inhibiting blue mould rot in the three yeasts. The effects of different concentrations of the three yeasts and addition Of nutrients, as well as combination with calcium on controlling blue mould rot in apples were presented in the paper. The results indicated that the higher the yeast concentrations, the more effective the yeasts on biocontrol activity. Addition of nutrients reduced the biocontrol capacity of the yeasts. Combination of 0.18 mol/L CaCl2 with C. laurentii cell suspensions could significantly enhance its effect to P. expansum in apple fruits, while the efficacy of calcium on biocontrol activity of T. pullulans or R. glutinis was not remarkable.展开更多
文摘目的探讨影响AIDS合并马尔尼菲青霉菌病（PSM）预后的因素。方法165例经病嗯学确诊的AIDS合并PSM患者，根据预岳分成牛存组和死亡组。应用Logistic回归，分析2j个可能影响疾病预后的临床和实验室指标．筛选出独立预后因素。结果AIDS合并PSM患者中。生有组121例，死亡组44例，病死率为26．7％。单因素分析显示．影响AIDS合并PSM预后的因素包括皮疹、细菌和（或）其他真菌性肺炎、CMV感染、败血症、肾功能损伤、PLT减少、Hb、CD4-T淋巴细胞计数、Alb、伊曲康唑（ITR）广子贯疗法及高效抗反转录病毒治疗（HAART）。多因素分析显示，去除各因素之间相互混杂的影响，筛选出细菌或其他真菌性甘市炎（OR=6．539；95％CI2．312，18．498）、败血症（OR=15．307；95%,CI1．473．42．462）及PLT减少（OR=7．763：95％CI2．658，22．675）是AIDS合并PSM死亡的危险因素，而ITR序贯疗法（OR=0．088；95％CI 0．013．0．611）与HAART（OR=0．084；95% CI 0．026，0．269）是其保护性因素。结论合并细菌或其他真菌性肺炎、败血症与PLT减少是AIDS合并PSM预后不良的危险因素，ITR序贯疗法和HAART则可减少死亡风险，改善预后；
基金UPM and the Fundamental Research Grant Scheme provided by Ministry of Higher Education under project no. 01-11-08-661FR for the financial support for some parts of this study
文摘Filamentous fungi are able to accumulate significant amount of metals from their environment. The potential of fungal biomass as agents for biosorption of heavy metals from contaminated sediments is currently receiving attention. In the present study, a total of 41 isolates of filamentous fungi obtained from the sediment of the Langat River, Selangor, Malaysia were screened for their tolerance and uptake capability of copper （Cu） and lead （Pb）. The isolates were identified as Aspergillus niger, A. fumigatus, Trichoderma asperellum, Penicillium simplicissimurn and P. janthinellum. A. niger and P. simplicissimum, were able to survive at 1000 mg/L of Cu（Ⅱ） concentration on Potato Dextrose Agar （PDA） while for Pb, only A. niger survived at 5000 mg/L concentration. The results showed that A. niger, P. simplicissimum and T. asperellum have a better uptake capacity for Pb compared to Cu and the findings indicated promising biosorption of Cu and Pb by these filamentous fungi from aqueous solution. The present study was also determined the maximum removal of Cu（Ⅱ） and Pb（Ⅱ） that was performed by A. niger. The metal removal which occurred at Cu（Ⅱ） 200 mg/L was （20.910 ＋ 0.581） mg/g and at 250 mg/L of PbⅡ） was （54.046 ± 0.328） mg/g.
文摘Three antagonistic yeasts, Trichosporon pullulans (Lindner.) Diddens and Lodder, Cryptococcus laurentii (Kuffer.) Skinner and Rhodotorula glutinis (Fresen.) F. C. Harrison, selected from over 50 yeasts on apple fruits, were investigated of their biocontrol efficacy against blue mould rot caused by Penicillium expansum Link in apple fruits. T. pullulans was identified to be a new yeast antagonist and was the most effective at inhibiting blue mould rot in the three yeasts. The effects of different concentrations of the three yeasts and addition Of nutrients, as well as combination with calcium on controlling blue mould rot in apples were presented in the paper. The results indicated that the higher the yeast concentrations, the more effective the yeasts on biocontrol activity. Addition of nutrients reduced the biocontrol capacity of the yeasts. Combination of 0.18 mol/L CaCl2 with C. laurentii cell suspensions could significantly enhance its effect to P. expansum in apple fruits, while the efficacy of calcium on biocontrol activity of T. pullulans or R. glutinis was not remarkable.
