In this paper,the biological function of PLK-1,the correlation between PLK-1 and tumors,and the latest research progress on PLK-1 inhibitors under study are reviewed,in order to provide references for the research and...In this paper,the biological function of PLK-1,the correlation between PLK-1 and tumors,and the latest research progress on PLK-1 inhibitors under study are reviewed,in order to provide references for the research and development of PLK-1 inhibitors.展开更多
目的观察急性白血病( AL)患者骨髓形态特征,分析 AL 患者骨髓细胞与 K562 细胞系中 Plk-1 的表达特征。方法选取在本院接受治疗的急性髓细胞白血病患者( AML)和急性淋巴细胞白血病患者( ALL),通过穿刺法观察骨髓细胞学形态特征;K562 传...目的观察急性白血病( AL)患者骨髓形态特征,分析 AL 患者骨髓细胞与 K562 细胞系中 Plk-1 的表达特征。方法选取在本院接受治疗的急性髓细胞白血病患者( AML)和急性淋巴细胞白血病患者( ALL),通过穿刺法观察骨髓细胞学形态特征;K562 传代细胞与 AL 骨髓细胞标本进行 PCR扩增及 Western Blot 表达鉴定。结果 ALL 与 AML 骨髓细胞均呈现类圆形或不规则形,细胞核呈圆形或类圆形;ALL 骨髓细胞相对 AML 较小,胞浆少,无明显颗粒及核仁, AML 胞浆丰富,有明显颗粒及核仁;AL 患者骨髓细胞组与 K562 细胞系的组间表达丰度值没有明显差异;有 AL 家族史、红细胞和血小板计数下降、血尿酸水平上升、没有 AL 用药史的患者 Plk-1 表达显著升高。结论 AL 骨髓细胞形态特征符合肿瘤恶转的特点,Plk-1 mRNA 在 AL 患者中发生了特异性表达。展开更多
PLK1(polo like kinase)是一类广泛存在于真核细胞中的丝/苏氨酸激酶,其结构及功能均十分保守。研究表明,plk1在启动、维持及完成有丝分裂中扮演重要角色,plk1磷酸化多种下游底物而实现其功能。已发现plk1基因在多种恶性肿瘤中存在过表...PLK1(polo like kinase)是一类广泛存在于真核细胞中的丝/苏氨酸激酶,其结构及功能均十分保守。研究表明,plk1在启动、维持及完成有丝分裂中扮演重要角色,plk1磷酸化多种下游底物而实现其功能。已发现plk1基因在多种恶性肿瘤中存在过表达并与某些肿瘤的生物学行为及预后相关,有望成为恶性肿瘤的又一新的标记物,阻断plk1表达可导致体外肿瘤细胞分裂停滞及凋亡。展开更多
AIM： Polo-like kinase 1 （PLK1） serine/threonine kinase plays a vital role in multiple phases of mitosis in gastric cancer cells. To investigate the effect of PLK1 depletion on mitosis and apoptosis of gastric cance...AIM： Polo-like kinase 1 （PLK1） serine/threonine kinase plays a vital role in multiple phases of mitosis in gastric cancer cells. To investigate the effect of PLK1 depletion on mitosis and apoptosis of gastric cancer cells. METHODS： PLK1 expression was blocked by small RNA interference（siRNA）. The expression levels of PLK1, cdc2, cyclin B and caspase 3 were detected by Western blotting. Then, PLK1 depletion, cdc2 activity, cell proliferation, cell cycle phase distribution, mitotic spindle structure, and the rate of apoptosis of the PLK1 knockdown cells were observed. RESULTS： PLK1 gene knockdown was associated with increased cyclin B expression, increased cdc2 activity （but not with the expression levels）, accumulation of gastric cancer cells at G2/M, improper mitotic spindle formation, delayed chromosome separation and delayed or arrested cytokinesis. Moreover, PLK1 depletion in gastric cancer cells was associated with decreased proliferation, attenuated pro-caspase 3 levels and increased apoptosis. CONCLUSION： Blockage of to decreased mitosis or even PLK1 expression may lead apoptosis in gastric cancer cells, indicating that PLK1 may be a valuable therapeutic target for gastric cancer.展开更多
文摘In this paper,the biological function of PLK-1,the correlation between PLK-1 and tumors,and the latest research progress on PLK-1 inhibitors under study are reviewed,in order to provide references for the research and development of PLK-1 inhibitors.
文摘目的观察急性白血病( AL)患者骨髓形态特征,分析 AL 患者骨髓细胞与 K562 细胞系中 Plk-1 的表达特征。方法选取在本院接受治疗的急性髓细胞白血病患者( AML)和急性淋巴细胞白血病患者( ALL),通过穿刺法观察骨髓细胞学形态特征;K562 传代细胞与 AL 骨髓细胞标本进行 PCR扩增及 Western Blot 表达鉴定。结果 ALL 与 AML 骨髓细胞均呈现类圆形或不规则形,细胞核呈圆形或类圆形;ALL 骨髓细胞相对 AML 较小,胞浆少,无明显颗粒及核仁, AML 胞浆丰富,有明显颗粒及核仁;AL 患者骨髓细胞组与 K562 细胞系的组间表达丰度值没有明显差异;有 AL 家族史、红细胞和血小板计数下降、血尿酸水平上升、没有 AL 用药史的患者 Plk-1 表达显著升高。结论 AL 骨髓细胞形态特征符合肿瘤恶转的特点,Plk-1 mRNA 在 AL 患者中发生了特异性表达。
文摘PLK1(polo like kinase)是一类广泛存在于真核细胞中的丝/苏氨酸激酶,其结构及功能均十分保守。研究表明,plk1在启动、维持及完成有丝分裂中扮演重要角色,plk1磷酸化多种下游底物而实现其功能。已发现plk1基因在多种恶性肿瘤中存在过表达并与某些肿瘤的生物学行为及预后相关,有望成为恶性肿瘤的又一新的标记物,阻断plk1表达可导致体外肿瘤细胞分裂停滞及凋亡。
基金Supported by the Major State Basic Research Development Program of China,973 program,No.2002CB713700
文摘AIM： Polo-like kinase 1 （PLK1） serine/threonine kinase plays a vital role in multiple phases of mitosis in gastric cancer cells. To investigate the effect of PLK1 depletion on mitosis and apoptosis of gastric cancer cells. METHODS： PLK1 expression was blocked by small RNA interference（siRNA）. The expression levels of PLK1, cdc2, cyclin B and caspase 3 were detected by Western blotting. Then, PLK1 depletion, cdc2 activity, cell proliferation, cell cycle phase distribution, mitotic spindle structure, and the rate of apoptosis of the PLK1 knockdown cells were observed. RESULTS： PLK1 gene knockdown was associated with increased cyclin B expression, increased cdc2 activity （but not with the expression levels）, accumulation of gastric cancer cells at G2/M, improper mitotic spindle formation, delayed chromosome separation and delayed or arrested cytokinesis. Moreover, PLK1 depletion in gastric cancer cells was associated with decreased proliferation, attenuated pro-caspase 3 levels and increased apoptosis. CONCLUSION： Blockage of to decreased mitosis or even PLK1 expression may lead apoptosis in gastric cancer cells, indicating that PLK1 may be a valuable therapeutic target for gastric cancer.
