Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, c...Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, carded by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carded out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes （Pia-1, Pia-2, Pia-3, and Pia-4）, all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene （CRG） markers （A17, A25, A26, and A27）, derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 （Pia-3） perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia.展开更多
Objective To evaluate the clinical efficacy of incising spinal pia mater to relieve pressure and unilateral open-door laminoplasty with internal screw fixation for treatment of the dated spinal cord injury. Methods Fr...Objective To evaluate the clinical efficacy of incising spinal pia mater to relieve pressure and unilateral open-door laminoplasty with internal screw fixation for treatment of the dated spinal cord injury. Methods From March, 2009 to July, 2010, 16 cases with chronic cervical cord injury underwent spinal dura mater incision and unilateral open-door laminoplasty with internal screw fixation. Nerve functions of preand postoperation were evaluated by Frankel classification and the Japanese Orthopaedic Association (JOA) scale. The improvement rate of JOA score at the indicated time was recorded. Results Postoperative Frankel classification rating of 16 patients improved obviously. JOA scores at the 1st month, 3rd month, 6th month, and 12th month after surgery were 7.9±2.3, 8.5±1.6, 8.9±2.1, and 12.4±2.5, respectively, and significantly increased compared with that prior to surgery (5.5±0.6). At the end of follow-up period, JOA score was significantly higher than that of pre-treatment (P<0.05). The recovery was relatively rapid during the first 3 months following the surgery, then entered a platform period. Conclusion It is effective for patients with dated spinal cord injury to undergo spinal decompression and laminoplasty.展开更多
id+c:请问,您作为PIA室内设计公司的掌舵者,一直以来秉持的设计理念是什么?Vachirapong Danny Arthayukti:作为PIA的负责人,我的设计理念是致力于提供高质量的服务,以获得客户的口碑传播,从而获取更多的工作机会。我们并不在营销方面大...id+c:请问,您作为PIA室内设计公司的掌舵者,一直以来秉持的设计理念是什么?Vachirapong Danny Arthayukti:作为PIA的负责人,我的设计理念是致力于提供高质量的服务,以获得客户的口碑传播,从而获取更多的工作机会。我们并不在营销方面大量投资,也不积极寻求客户。我们之所以能够做到这一点,是因为我们在工作中投入了全部的热情,竭尽所能地完成好每一个项目。展开更多
基金supported by the National Transgenic Research Projects (Grant No.2009ZX08009-023B)the National Basic Research Program of China(Grant No.2011CB1007)the National Commonweal Specialized Research Project(Grant No.200803008)
文摘Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, carded by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carded out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes （Pia-1, Pia-2, Pia-3, and Pia-4）, all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene （CRG） markers （A17, A25, A26, and A27）, derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 （Pia-3） perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia.
文摘Objective To evaluate the clinical efficacy of incising spinal pia mater to relieve pressure and unilateral open-door laminoplasty with internal screw fixation for treatment of the dated spinal cord injury. Methods From March, 2009 to July, 2010, 16 cases with chronic cervical cord injury underwent spinal dura mater incision and unilateral open-door laminoplasty with internal screw fixation. Nerve functions of preand postoperation were evaluated by Frankel classification and the Japanese Orthopaedic Association (JOA) scale. The improvement rate of JOA score at the indicated time was recorded. Results Postoperative Frankel classification rating of 16 patients improved obviously. JOA scores at the 1st month, 3rd month, 6th month, and 12th month after surgery were 7.9±2.3, 8.5±1.6, 8.9±2.1, and 12.4±2.5, respectively, and significantly increased compared with that prior to surgery (5.5±0.6). At the end of follow-up period, JOA score was significantly higher than that of pre-treatment (P<0.05). The recovery was relatively rapid during the first 3 months following the surgery, then entered a platform period. Conclusion It is effective for patients with dated spinal cord injury to undergo spinal decompression and laminoplasty.
文摘id+c:请问,您作为PIA室内设计公司的掌舵者,一直以来秉持的设计理念是什么?Vachirapong Danny Arthayukti:作为PIA的负责人,我的设计理念是致力于提供高质量的服务,以获得客户的口碑传播,从而获取更多的工作机会。我们并不在营销方面大量投资,也不积极寻求客户。我们之所以能够做到这一点,是因为我们在工作中投入了全部的热情,竭尽所能地完成好每一个项目。
