Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including...Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including the ER for specific structural and functional needs.Peroxisomes are recently found to receive cholesterol from lysosomes through lysosomeperoxisome membrane contacts.However,whether and how cholesterol is conveyed from peroxisomes to the ER remain unknown.Here,by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes,we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(4,5)P2-containing liposomes to the ER in vitro,and that the presence of peroxisomes augments cholesterol transfer from lysosomes to the ER.Together,our study reveals a new cholesterol transport pathway along the lysosome-peroxisome-ER membrane contacts in the cell.展开更多
针对大型气象监测站成本高、覆盖范围有限、灵活性差的问题,设计了一套面向局部定点区域的智能化微型气象监测系统。首先,基于树莓派Raspberry Pi 4B平台开发了一套微型气象数据采集装置,可实现多种气象参数的实时采集与传输。然后,基...针对大型气象监测站成本高、覆盖范围有限、灵活性差的问题,设计了一套面向局部定点区域的智能化微型气象监测系统。首先,基于树莓派Raspberry Pi 4B平台开发了一套微型气象数据采集装置,可实现多种气象参数的实时采集与传输。然后,基于Qt开发了一款服务端软件,实现了针对前端采集气象数据的接收、存储、管理和可视化显示。前端采集系统实现了多种气象信息的综合检测,且系统功能可靠稳定;服务端系统实现的大数据管理和图形化显示,增强了系统可读性和实用性。该系统不仅能为气象数据的联合分析提供可靠数据源,也为业界开发相关商业产品提供了借鉴思路。展开更多
针对木勺模压生产线,生产过程中一次性木勺在模具中产生遗料现象,利用相关图像识别技术,设计了一种在木勺生产过程中使用的遗料识别系统。以Raspberry Pi 4B作为控制板,利用Python设计编写相关遗料识别程序。利用该程序对相关图像进行...针对木勺模压生产线,生产过程中一次性木勺在模具中产生遗料现象,利用相关图像识别技术,设计了一种在木勺生产过程中使用的遗料识别系统。以Raspberry Pi 4B作为控制板,利用Python设计编写相关遗料识别程序。利用该程序对相关图像进行霍夫圆检测以及透视变换的处理,借以帧差法对比图像与图像之间的差异从而判断模具中有无遗料。实验证明,编写的遗料识别程序可以满足预期生产要求,程序的准确率接近100%,稳定性好。展开更多
Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effe...Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are available.Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections.In this study,we demonstrated that curcumin showed potent antiviral effect again EV71.In Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral progeny.Similar with the previous reports,curcumin reduced the production of ROS induced by viral infection.However,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral replication.This study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of curcumin.Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control cells.However,curcumin did reduce the activity of proteasomes which was increased by viral infection.In addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected cells.We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication complex.We found that curcumin significantly reduced the level of both proteins.Moreover,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral replication.We also demonstrated that curcumin showed anti-apoptotic展开更多
布匹瑕疵检测是纺织业质量管理的重要环节.在嵌入式设备上实现准确、快速的布匹瑕疵检测能有效降低成本,因而价值巨大.考虑到实际生产中花色布匹瑕疵具有背景复杂、数量差异大、极端长宽比和小瑕疵占比高等结构特性,提出一种基于轻量级...布匹瑕疵检测是纺织业质量管理的重要环节.在嵌入式设备上实现准确、快速的布匹瑕疵检测能有效降低成本,因而价值巨大.考虑到实际生产中花色布匹瑕疵具有背景复杂、数量差异大、极端长宽比和小瑕疵占比高等结构特性,提出一种基于轻量级模型的花色布匹瑕疵检测方法并将其部署在嵌入式设备Raspberry Pi 4B上.首先在一阶段目标检测网络YOLO的基础上用轻量级特征提取网络ShuffleNetV2提取花色布匹瑕疵的特征,以减少网络结构复杂度及参数量,提升检测速度;其次是检测头的解耦合,将分类与定位任务分离,以提升模型收敛速度;此外引入CIoU作为瑕疵位置回归损失函数,提高瑕疵定位准确性.实验结果表明,本文算法在Raspberry Pi 4B上可达8.6 FPS的检测速度,可满足纺织工业应用需求.展开更多
Applying dynamic web services composition is one of the important schemas for solving service-oriented architecture （SOA）and service-oriented computing（SOC）.For implementing dynamic web services composition,the mu...Applying dynamic web services composition is one of the important schemas for solving service-oriented architecture （SOA）and service-oriented computing（SOC）.For implementing dynamic web services composition,the multi agent system （MAS）is applied to web services composition.First, the essentials of the MAS and web services composition are analyzed and their relationship is discussed.Secondly, an MAS-based architecture is designed for dynamic web services composition,and it is named as CSMWC.A Jade tool is used to implement the major components.The architecture can primarily implement syntactic level dynamic web services composition,and it gives a basis for semantic level dynamic web services composition.For specifying the correctness of the architecture,by using pi-calculus,the architecture of the MAS is formally described,and its dynamic properties and adaptability are reasoned.Finally,it demonstrates the idea proposed by the Pi4SOA tool.展开更多
基金supported by the National Natural Science Foundation of China (91754102, 31771568, 31690102, 31600651, 31701030)National Key Research and Development Project of the Ministry of Science and Technology of China (2016YFA0500100)+2 种基金Shenzhen City Technology Basic Research Program (JCYJ20170818144026198)Science and Technology Department of Hubei Province (2017CFB617)the 111 Project of Ministry of Education of China (B16036)
文摘Most mammalian cells take up cholesterol from low-density lipoproteins(LDLs) via receptor-mediated endocytosis.After reaching lysosomes,LDL-derived cholesterol continues to transport to downstream organelles including the ER for specific structural and functional needs.Peroxisomes are recently found to receive cholesterol from lysosomes through lysosomeperoxisome membrane contacts.However,whether and how cholesterol is conveyed from peroxisomes to the ER remain unknown.Here,by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes,we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1,2 and 3(E-Syts).Depletion of peroxisomal PI(4,5)P2 or ESyts markedly decreases peroxisome-ER membrane contacts and induces cholesterol accumulation in lysosomes.Furthermore,we show that cholesterol is delivered from 3H-labeled peroxisomes or PI(4,5)P2-containing liposomes to the ER in vitro,and that the presence of peroxisomes augments cholesterol transfer from lysosomes to the ER.Together,our study reveals a new cholesterol transport pathway along the lysosome-peroxisome-ER membrane contacts in the cell.
文摘针对大型气象监测站成本高、覆盖范围有限、灵活性差的问题,设计了一套面向局部定点区域的智能化微型气象监测系统。首先,基于树莓派Raspberry Pi 4B平台开发了一套微型气象数据采集装置,可实现多种气象参数的实时采集与传输。然后,基于Qt开发了一款服务端软件,实现了针对前端采集气象数据的接收、存储、管理和可视化显示。前端采集系统实现了多种气象信息的综合检测,且系统功能可靠稳定;服务端系统实现的大数据管理和图形化显示,增强了系统可读性和实用性。该系统不仅能为气象数据的联合分析提供可靠数据源,也为业界开发相关商业产品提供了借鉴思路。
文摘针对木勺模压生产线,生产过程中一次性木勺在模具中产生遗料现象,利用相关图像识别技术,设计了一种在木勺生产过程中使用的遗料识别系统。以Raspberry Pi 4B作为控制板,利用Python设计编写相关遗料识别程序。利用该程序对相关图像进行霍夫圆检测以及透视变换的处理,借以帧差法对比图像与图像之间的差异从而判断模具中有无遗料。实验证明,编写的遗料识别程序可以满足预期生产要求,程序的准确率接近100%,稳定性好。
基金This work was supported by the grants of National Natural Science Foundation of China to Zhaohua Zhong(Grant No.81271825)Wenran Zhao(Grant No.31270198).
文摘Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are available.Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections.In this study,we demonstrated that curcumin showed potent antiviral effect again EV71.In Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral progeny.Similar with the previous reports,curcumin reduced the production of ROS induced by viral infection.However,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral replication.This study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of curcumin.Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control cells.However,curcumin did reduce the activity of proteasomes which was increased by viral infection.In addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected cells.We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication complex.We found that curcumin significantly reduced the level of both proteins.Moreover,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral replication.We also demonstrated that curcumin showed anti-apoptotic
文摘布匹瑕疵检测是纺织业质量管理的重要环节.在嵌入式设备上实现准确、快速的布匹瑕疵检测能有效降低成本,因而价值巨大.考虑到实际生产中花色布匹瑕疵具有背景复杂、数量差异大、极端长宽比和小瑕疵占比高等结构特性,提出一种基于轻量级模型的花色布匹瑕疵检测方法并将其部署在嵌入式设备Raspberry Pi 4B上.首先在一阶段目标检测网络YOLO的基础上用轻量级特征提取网络ShuffleNetV2提取花色布匹瑕疵的特征,以减少网络结构复杂度及参数量,提升检测速度;其次是检测头的解耦合,将分类与定位任务分离,以提升模型收敛速度;此外引入CIoU作为瑕疵位置回归损失函数,提高瑕疵定位准确性.实验结果表明,本文算法在Raspberry Pi 4B上可达8.6 FPS的检测速度,可满足纺织工业应用需求.
基金The National Natural Science Foundation of China(No.90612014)IBM China Research Lab Joint Project
文摘Applying dynamic web services composition is one of the important schemas for solving service-oriented architecture （SOA）and service-oriented computing（SOC）.For implementing dynamic web services composition,the multi agent system （MAS）is applied to web services composition.First, the essentials of the MAS and web services composition are analyzed and their relationship is discussed.Secondly, an MAS-based architecture is designed for dynamic web services composition,and it is named as CSMWC.A Jade tool is used to implement the major components.The architecture can primarily implement syntactic level dynamic web services composition,and it gives a basis for semantic level dynamic web services composition.For specifying the correctness of the architecture,by using pi-calculus,the architecture of the MAS is formally described,and its dynamic properties and adaptability are reasoned.Finally,it demonstrates the idea proposed by the Pi4SOA tool.
