AIM To study the anticancer effect of artesunate and its mechanism. METHODS To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl 2 and Bax genes in mice bearing H 22 solid h...AIM To study the anticancer effect of artesunate and its mechanism. METHODS To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl 2 and Bax genes in mice bearing H 22 solid hepatic carcinoma. RESULTS After administration of artesunate (ig, 300 mg·kg -1 ·d -1 ×7 d), growth of solid tumor was obviously inhibited, the tumor inhibitory rates were 49 1%, 48 7% and 46 6% in 3 experiments. The apoptosis of liver cancer cells were increased. Immunohistochemical staining showed that the number of Bcl 2 protein positive cells were decreased, but the number of Bax protein positive cells were increased. The PCNA positive cells were significantly lower than those in the control group. CONCLUSION Artesunate showed obvious anticancer activity on H 22 hepatic carcinoma bearing mice and undergo apoptosis of liver cancer cells. The mechanism of anticancer effect of artesunate may be related to down regulation of the expression of PCNA and Bcl 2 genes and up regulation of the expression of Bax gene.展开更多
AIM: To characterize the alteration and significance of p53and PCNA in cancer and adjacent tissues of concurrent cancersfrom the esophagus and gastric cardia in the same patient.METHODS: P53 and PCNA protein accumulat...AIM: To characterize the alteration and significance of p53and PCNA in cancer and adjacent tissues of concurrent cancersfrom the esophagus and gastric cardia in the same patient.METHODS: P53 and PCNA protein accumulation in 25patients with concurrent cancers from the esophagus andgastric cardia (CC, concurrent carcinomas of esophagealsquamous cell carcinoma and gastric cardia adenocarcinoma)were detected by immunohistochemical method (ABC).RESULTS: In CC patients, both esophageal squamous cellcarcinoma (SCC) and gastric cardia adenocarcinoma (GCA)tissues showed different positive immunostaining extent ofp53 and PCNA protein (P＞0.05). The positive immunostainingrates for p53 and PCNA were 60 % (15/25) and 92 % (23/25), respectively in SCC; and 40 % (10/25) and 88 % (22/25), respectively in GCA. 'Diffuse' immunostaining patternwas frequently observed in both p53 and PCNA. Highcoincidence rates for p53 and PCNA positive staining wereobserved in SCC and GCA from the same patients, andaccounted for 56 % and 96 %. In SCC patients, with thelesions progressed from normal esophageal epithelium (NOR)to basal cell hyperplasia (BCH) to dysplasia (DYS) tocarcinomain situ (CIS) to SCC, the positive rates for p53were 27 %, 50 %, 50 %, 29 % and 72 %, and 55 %, 70 %,75 %, 71% and 93 % for PCNA, respectively. In GCA, withthe lesions progressed from normal gastric cardia epitheliumto DYS to CIS to GCA, the positive rates of p53 expressionwere 44 %, 27 %, 22 % and 36 % respectively, the differencewas not significant; the positive rates of PCNA proteinexpression were 67 %, 64 %, 67 % and 86 %, respectively.The x2 test, Fisher's Exact Test, Mantel-Haenszel x2 Testand Kappa Test were used for the statistics.CONCLUSION: The high coincident alterations for P53 andPCNA in SCC and GCA from the same patient indicate thepossibility of similar molecular basis, which providesimportant molecular basis and etiological clue for similargeographic distribution and risk factors in SCC and GCA.展开更多
文摘AIM To study the anticancer effect of artesunate and its mechanism. METHODS To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl 2 and Bax genes in mice bearing H 22 solid hepatic carcinoma. RESULTS After administration of artesunate (ig, 300 mg·kg -1 ·d -1 ×7 d), growth of solid tumor was obviously inhibited, the tumor inhibitory rates were 49 1%, 48 7% and 46 6% in 3 experiments. The apoptosis of liver cancer cells were increased. Immunohistochemical staining showed that the number of Bcl 2 protein positive cells were decreased, but the number of Bax protein positive cells were increased. The PCNA positive cells were significantly lower than those in the control group. CONCLUSION Artesunate showed obvious anticancer activity on H 22 hepatic carcinoma bearing mice and undergo apoptosis of liver cancer cells. The mechanism of anticancer effect of artesunate may be related to down regulation of the expression of PCNA and Bcl 2 genes and up regulation of the expression of Bax gene.
基金National Outstanding Young Scientist Award of China, No.30025016Foundation of Henan Education Committee
文摘AIM: To characterize the alteration and significance of p53and PCNA in cancer and adjacent tissues of concurrent cancersfrom the esophagus and gastric cardia in the same patient.METHODS: P53 and PCNA protein accumulation in 25patients with concurrent cancers from the esophagus andgastric cardia (CC, concurrent carcinomas of esophagealsquamous cell carcinoma and gastric cardia adenocarcinoma)were detected by immunohistochemical method (ABC).RESULTS: In CC patients, both esophageal squamous cellcarcinoma (SCC) and gastric cardia adenocarcinoma (GCA)tissues showed different positive immunostaining extent ofp53 and PCNA protein (P＞0.05). The positive immunostainingrates for p53 and PCNA were 60 % (15/25) and 92 % (23/25), respectively in SCC; and 40 % (10/25) and 88 % (22/25), respectively in GCA. 'Diffuse' immunostaining patternwas frequently observed in both p53 and PCNA. Highcoincidence rates for p53 and PCNA positive staining wereobserved in SCC and GCA from the same patients, andaccounted for 56 % and 96 %. In SCC patients, with thelesions progressed from normal esophageal epithelium (NOR)to basal cell hyperplasia (BCH) to dysplasia (DYS) tocarcinomain situ (CIS) to SCC, the positive rates for p53were 27 %, 50 %, 50 %, 29 % and 72 %, and 55 %, 70 %,75 %, 71% and 93 % for PCNA, respectively. In GCA, withthe lesions progressed from normal gastric cardia epitheliumto DYS to CIS to GCA, the positive rates of p53 expressionwere 44 %, 27 %, 22 % and 36 % respectively, the differencewas not significant; the positive rates of PCNA proteinexpression were 67 %, 64 %, 67 % and 86 %, respectively.The x2 test, Fisher's Exact Test, Mantel-Haenszel x2 Testand Kappa Test were used for the statistics.CONCLUSION: The high coincident alterations for P53 andPCNA in SCC and GCA from the same patient indicate thepossibility of similar molecular basis, which providesimportant molecular basis and etiological clue for similargeographic distribution and risk factors in SCC and GCA.
